Biocompatible and Bioactive Surface Modifications for Prolonged In Vivo Efficacy

Meyers, Steven R.; Grinstaff, Mark W.

doi:10.1021/cr2000916

Cited by 232 publications

(188 citation statements)

References 125 publications

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“…As mentioned previously, the ability to control biomolecule and cell-material interactions in an environment that mimics properties of the extracellular matrix is an important goal for a range of biomedical applications, in vitro and in vivo [23]. Undesired cell responses can be triggered by non-specific protein adsorption onto the surface which can be problematic in the control of specific receptor-ligand interactions, such those involved in cell signalling pathways [24].…”

Section: Resultsmentioning

confidence: 99%

Surface grafting of electrospun fibers using ATRP and RAFT for the control of biointerfacial interactions

et al. 2013

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Background The ability to present signalling molecules within a low fouling 3D environment that mimics the extracellular matrix is an important goal for a range of biomedical applications, both in vitro and in vivo. Cell responses can be triggered by non-specific protein interactions occurring on the surface of a biomaterial, which is an undesirable process when studying specific receptor-ligand interactions. It is therefore useful to present specific ligands of interest to cell surface receptors in a 3D environment that minimizes non-specific interactions with biomolecules, such as proteins. Method In this study, surface-initiated atom transfer radical polymerization (SI-ATRP) of poly(ethylene glycol)-based monomers was carried out from the surface of electrospun fibers composed of a styrene/vinylbenzyl chloride copolymer. Surface initiated radical addition-fragmentation chain transfer (SI-RAFT) polymerisation was also carried out to generate bottle brush copolymer coatings consisting of poly(acrylic acid) and poly(acrylamide). These were grown from surface trithiocarbonate groups generated from the chloromethyl styrene moieties existing in the original synthesised polymer. XPS was used to characterise the surface composition of the fibers after grafting and after coupling with fluorine functional XPS labels. Results Bottle brush type coatings were able to be produced by ATRP which consisted of poly(ethylene glycol) methacrylate and a terminal alkyne-functionalised monomer. The ATRP coatings showed reduced non-specific protein adsorption, as a result of effective PEG incorporation and pendant alkynes groups existing as part of the brushes allowed for further conjugation of via azide-alkyne Huisgen 1,3-dipolar cycloaddition. In the case of RAFT, carboxylic acid moieties were effectively coupled to an amine label via amide bond formation. In each case XPS analysis demonstrated that covalent immobilisation had effectively taken place. Conclusion Overall, the studies presented an effective platform for the preparation of 3D scaffolds which contain effective conjugation sites for attachment of specific bioactive signals of interest, as well as actively reducing non-specific protein interactions.

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Section: Resultsmentioning

confidence: 99%

Surface grafting of electrospun fibers using ATRP and RAFT for the control of biointerfacial interactions

et al. 2013

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“…Although increasing PEG size correlates with enhanced bioinert properties, short ethylene glycol units can induce cell-repellent effects as well (Banerjee et al, 2011;Pagel et al, 2016). Surfaces have been coated with PEG by various immobilization methods, such as chemisorption, physisorption and covalent strategies which is well-reviewed elsewhere (Meyers and Grinstaff, 2012). In addition to the already described non-fouling properties of polysaccharides, peptides (mostly zwitterionic) are known to mitigate unspecific protein and bacteria adhesion (Cui et al, 2014;Maity et al, 2014;Yu et al, 2015).…”

Section: Components Of Multifunctional Biomaterials Coatingsmentioning

confidence: 99%

Multifunctional biomaterial coatings: synthetic challenges and biological activity

Pagel

Beck‐Sickinger

2017

Biological Chemistry

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A controlled interaction of materials with their surrounding biological environment is of great interest in many fields. Multifunctional coatings aim to provide simultaneous modulation of several biological signals. They can consist of various combinations of bioactive, and bioinert components as well as of reporter molecules to improve cell-material contacts, prevent infections or to analyze biochemical events on the surface. However, specific immobilization and particular assembly of various active molecules are challenging. Herein, an overview of multifunctional coatings for biomaterials is given, focusing on synthetic strategies and the biological benefits by displaying several motifs.

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“…2 In addition to drug delivery, PEG has been intensively explored as biocompatible, anti-fouling coatings for medical implants to improve the in vivo efficacies. 3 The work reported here is a natural follow-up of our bioenginering effort involving the surface modification of cochlear implants for enhanced electro-neural interfacing 4 . PEGylation was conducted on platinum electrodes to render the surface protein-resistant.…”

Section: Introductionmentioning

confidence: 99%

PEGylation of platinum bio-electrodes

Yue

Molino

Liu

et al. 2013

Electrochemistry Communications

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Controlling protein interactions at the implanted electrode interface is becoming an important strategy for the management of foreign body responses that have proven to be detrimental to the long-term performance of neural prosthesis. In this study, PEGylation was conducted on platinum bio-electrodes to render the surface protein-resistant. The PEGylated electrode was investigated using a quartz crystal microbalance-dissipation, electrochemical impedance spectroscopy and cyclic voltammetiy. AbstractControlling protein interactions at the implanted electrode interface is becoming an important strategy for the management of foreign body responses that have proven to be detrimental to the long-term performance of neural prosthesis. In this study, PEGylation was conducted on platinum bio-electrodes to render the surface protein-resistant. The PEGylated electrode was investigated using a quartz crystal microbalance-dissipation, electrochemical impedance spectroscopy and cyclic voltammetry.

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Biocompatible and Bioactive Surface Modifications for Prolonged In Vivo Efficacy

Cited by 232 publications

References 125 publications

Surface grafting of electrospun fibers using ATRP and RAFT for the control of biointerfacial interactions

Surface grafting of electrospun fibers using ATRP and RAFT for the control of biointerfacial interactions

Multifunctional biomaterial coatings: synthetic challenges and biological activity

PEGylation of platinum bio-electrodes

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