Biocompatibility studies of natural rubber latex from different tree clones and collection methods

Abstract

Natural rubber latex (NRL) has several features that make it an excellent biomaterial to promote the growth and repair of tissues, skin and bones. Most of the research with NRL membranes uses a mixture of different clones and chemical preservatives in the collection process. In this study, we compared five clones that produce NRL, seeking to identify their differences in biocompatibility. The clones studied were RRIM 600, PB 235, GT1, PR 255 and IAN 873 commonly found in plantations in Brazil. We did also study the effect of ammonia used during latex collection. NRL membranes were prepared aseptically and sterilized. In the in vitro tests, the membranes remained in direct contact with mouse fibroblasts cells for three periods, 24, 48 and 72 h. In the in vivo tests, the membranes were implanted subcutaneously in rabbits. The results indicated the biocompatibility of the membranes obtained from all clones. Membranes from the clones RRIM 600 and IAN 873 induced greater cell proliferation, suggesting greater bioactivity. It was found that the membranes made from latex that was in contact with ammonia during collection, showed cytotoxic and genotoxic effects in cultures, as well as necrosis, and increased inflammatory cells in the rabbit's tissues close to the implant.

Polyurethane derived from Ricinus Communis as graft for bone defect treatments

“…Its use as an occlusive membrane for bone regeneration has already been tested in animal models, with satisfactory results [23,24] . Other literature data indicate its biocompatibility [25,26] , its angiogenic potential [21,27,28] and its ability to act as a drug delivery system [29][30][31] . Thus, the objective of this work was to study the effect of polyurethane granules (Polyquil  ) as graft material in bone regeneration cases.…”

Efecto de inclusión de anticoagulantes durante el beneficio de Hevea brasiliensis (Willd. ex A.Juss.) Müll.Arg.

“…La adición de anticoagulantes no es un proceso nuevo, este proceso se le conoce como tapping, usualmente se adiciona amoníaco en las tazas de recolección del látex, ya que evita la coagulación y es bactericida, muy utilizado para la producción de látex preservado, centrifugado y cremado, utilizado en la fabricación de globos, guantes, condones, moldes entre otros (Camacho et al, 2014;Floriano et al, 2014;Tamara et al, 2015).…”

Comparative study of bone tissue accelerated regeneration by latex membranes from Hevea brasiliensis and Hancornia speciosa

“…In particular, the use of latex or natural rubber (NR) membranes obtained from the sap of the rubber tree (Hevea brasiliensis) has shown high osteogenic potential [4]. This is due to its characteristics of biocompatibility and high angiogenic potential, as well as the flexibility and mechanical stability, surface porosity, and permeability of the biomembranes [5,6].…”