Biocompatibility of wollastonite‐poly(<i>N</i>‐butyl‐2‐cyanoacrylate) composites

Sena, Lídia Ágata de; Almeida, Marcelo Sanmartin de; Fernandes, Gustavo Vicentis de Oliveira; Bretaña, Rosa Mayelín Guerra; Castro-Silva, Igor Iuco; Granjeiro, José Mauro; Achete, Carlos A.

doi:10.1002/jbm.b.33093

Cited by 12 publications

(10 citation statements)

References 16 publications

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“…Recently, the calcium silicate (CaSiO 3 , CS) ceramics and their composites are investigated as potentially new type of bioceramics for bone regeneration and bone tissue engineering applications [2][3][4][5][6][7][8][9][10][11][12][13][14]. Previous studies have confirmed that the CS bioceramics possess excellent bioactivity and biodegradability [12], and in vitro cell culture studies have shown that the CS bioceramics well supported the attachment and proliferation of bone marrow mesenchymal stem cells (BMSCs) and osteoblasts [11,13,15], and the bioactive Si ions released from CS provided a preferable extracellular environment for directing BMSCs proliferation and differentiation toward the osteogenic lineage [9], and enhancing the proliferation and angiogenesis process of the human umbilical vein endothelial cells (HUVECs) [3].…”

Section: Introductionmentioning

confidence: 99%

Degradation and silicon excretion of the calcium silicate bioactive ceramics during bone regeneration using rabbit femur defect model

Lin

Liu

Huang

et al. 2015

J Mater Sci: Mater Med

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The investigation of the bone regeneration ability, degradation and excretion of the grafts is critical for development and application of the newly developed biomaterials. Herein, the in vivo bone-regeneration, biodegradation and silicon (Si) excretion of the new type calcium silicate (CaSiO3, CS) bioactive ceramics were investigated using rabbit femur defect model, and the results were compared with the traditional β-tricalcium phosphate [β-Ca3(PO4)2, β-TCP] bioceramics. After implantation of the scaffolds in rabbit femur defects for 4, 8 and 12 weeks, the bone regenerative capacity and degradation were evaluated by histomorphometric analysis. While urine and some organs such as kidney, liver, lung and spleen were resected for chemical analysis to determine the excretion of the ionic products from CS implants. The histomorphometric analysis showed that the bioresorption rate of CS was similar to that of β-TCP in femur defect model, while the CS grafts could significantly stimulate bone formation capacity as compared with β-TCP bioceramics (P < 0.05). The chemical analysis results showed that Si concentration in urinary of the CS group was apparently higher than that in control group of β-TCP. However, no significant increase of the Si excretion was found in the organs including kidney, which suggests that the resorbed Si element is harmlessly excreted in soluble form via the urine. The present studies show that the CS ceramics can be used as safe, bioactive and biodegradable materials for hard tissue repair and tissue engineering applications.

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Section: Introductionmentioning

confidence: 99%

Degradation and silicon excretion of the calcium silicate bioactive ceramics during bone regeneration using rabbit femur defect model

Lin

Liu

Huang

et al. 2015

J Mater Sci: Mater Med

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“…The tissue adhesive used in the present study is a blend of n-butyl cyanoacrylate and 2-octyl cyanoacrylate and studies that have attempted to evaluate the cytotoxicity of adhesives with similar composition, showed a level of cytotoxicity around 40% [34] 60% [31,35] which is higher than the values found for shorth length alkyl chains [31,34]. In the present study the cell viability was 39.66%, which was similar to ndings of previous reports of similar adhesives, and lower when compared with the ISO 10993-12 speci cation that considers 70% to be the minimum requirement for toxicity in biomedical materials [34].…”

Section: Discussionmentioning

confidence: 99%

Cyanoacrylate Tissue Adhesive as a Membrane in Socket Preservation: In Vitro Cytotoxicity and Clinical Evaluation

Azpur

ortiz

Valdivia

et al. 2020

Preprint

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Background: Cyanoacrylate-based adhesives provide immediate hemostasis when applied; this is attributed to their ability to form a mechanical barrier in the surgical site, favoring the coagulation process and allowing control of bleeding. The purpose of the present study was to demonstrate the in vitro effect of cyanoacrylate-based tissue adhesive and the clinical evaluation of wound healing of freshly extracted sockets with adhesives applied on an exposed collagen membrane, for preservation of the alveolar ridge. Methods: Cytotoxicity was evaluated with sulphoradamine B assay with primary fibroblasts from gingival tissue. The in vivo analysis was carried out in 10 patients needing extraction of single-rooted teeth, who met the inclusion and exclusion criteria and signed the term of free and informed consent. Alveolar preservation was carried out with a mineralized cortical allograft bone substitute and a resorbable collagen membrane that was intentionally left exposed. On the exposed membrane, the Periacryl® 90 HV was applied as a barrier. The biocompatibility of the adhesive was assessed by the Early-Wound Healing Index (EHI) and Early Wound Healing Score (EHS), of perialveolar tissues at time intervals of 12, 30- and 60-days post-surgery. Results: Reduced cell viability was observed for Periacryl® 90 HV when compared with cells without adhesive treatments. No postoperative complications were observed after the application of Periacryl® 90 HV on perialveolar tissues. Conclusion: The use of the Periacryl® 90 HV cyanoacrylate-based tissue adhesive resulted in reduced fibroblast viability in vitro, and adequate results of wound closure in the clinical evaluation of EHI and EHS scores.

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“…Many studies have described the use of NBCA as a tissue adhesive. NBCA has been used with variable success rates for the treatment of bone fractures, ossicular fixation, embolization, fistulas, and hemostasis of esophageal varices or bleeding gastric ulcers as well as for vascular or tracheal anastomoses [71316171819202122232425]. These remarkable studies attempted to show the potential applications of NBCA.…”

Section: Discussionmentioning

confidence: 99%

Biocompatibility of n-butyl-2-cyanoacrylate (Histoacryl) in cervical structures of rats: prospective in vivo study

Suh

Kim

et al. 2019

Ann Surg Treat Res

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Purpose We investigated the biocompatibility of n-butyl-2-cyanoacrylate (NBCA) in the cervical deep tissues of rats to assess its biocompatibility. Methods A total of 30 Sprague-Dawley rats were injected with NBCA. After 30, 90, 180, and 360 days, cubes of tissue (1 cm × 1 cm × 1 cm) surrounding the NBCA and normal tissue from the other side of the neck were excised from each rat. The adhesion of NBCA to adjacent structures was examined histologically. Cells were counted per high-power field (HPF), and fibrosis was graded with the measurement of fibrotic thickening. Results All animals displayed normal behavior without any symptoms of distress throughout the study. There was no recognizable inflammatory reaction, foreign body reaction, or fibrosis in the 30 control samples. The analyses of experimental samples showed significantly decreased inflammatory cell counts over time (lymphoplasma cell count decreased from 100 (range, 70–100) to 30 (range, 30–50) per HPF, P = 0.010; neutrophil count decreased from 2 (range, 2–30) to 0 (range, 0–2) per HPF, P = 0.017). However, there was no significant difference in the number of multinuclear giant cells throughout the study period (a decrease from 22 [range, 16–34] to 16 [range, 12–22] per HPF, P = 0.287). The level of fibrosis was Common Toxicity Criteria ver. 4.0 Grade 1 without further thickening (P = 0.600). However, maturation of fibrosis progressed gradually. Conclusion NBCA was biologically tolerable in the cervical deep tissues of rats. However, precautions are needed with respect to preventing a sustained foreign body reaction and fibrosis.

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Biocompatibility of wollastonite‐poly(N‐butyl‐2‐cyanoacrylate) composites

Cited by 12 publications

References 16 publications

Degradation and silicon excretion of the calcium silicate bioactive ceramics during bone regeneration using rabbit femur defect model

Degradation and silicon excretion of the calcium silicate bioactive ceramics during bone regeneration using rabbit femur defect model

Cyanoacrylate Tissue Adhesive as a Membrane in Socket Preservation: In Vitro Cytotoxicity and Clinical Evaluation

Biocompatibility of n-butyl-2-cyanoacrylate (Histoacryl) in cervical structures of rats: prospective in vivo study

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