Biocompatibility of two calcium hydroxide-based endodontic sealers: A quantitative study in the subcutaneous connective tissue of the rat

Zmener, Osvaldo; Guglielmotti, M.B.; Cabrini, R. L.

doi:10.1016/s0099-2399(88)80175-4

Cited by 58 publications

(60 citation statements)

References 24 publications

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“…A great number of neutrophils and lymphocytes could be observed as well; and the number of macrophages also increased, and this was only seen in the IG, probably because of the great antigenicity of iodoform (10,12,14). In the CHG, there were few inflammatory cells; a great number could only be seen eleven days after the medication was inserted, in the repair stage, according to what has been found in other investigations (14,20). All in all, the inflammatory action of iodoform in this study had a peak period of five days after the drug insertion and the inflammatory process tended to establish a chronification after eleven days.…”

Section: • 3 Dayssupporting

confidence: 70%

“…The method used for evaluation was the qualitative and quantitative histological tissues' reaction analysis to the drugs on the back of rats (19,20). The animals were randomly distributed into four groups.…”

Section: Methodsmentioning

confidence: 99%

“…This medication may act by eliminating the contamination and/ or creating unsuitable conditions for the development and establishment of these microorganisms and by stimulating the host inflammatory response in order to eliminate the aggression factors (7)(8)(9)11,13,15). In this investigation, iodoform and calcium hydroxide were inserted in the back of rats, so that the action of these drugs on tissue could be investigated (19,20). In this study, the performance of iodoform (IG) was significantly better than the one of calcium hydroxide (CHG).…”

Section: • 3 Daysmentioning

confidence: 99%

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Tissue inflammatory response to implantation of calcium hydroxide and iodoform in the back of rats

Pallotta¹,

Machado²,

Reis³

et al. 2010

Rev. odonto ciênc. (Online)

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Purpose: This study evaluated the inflammatory reaction caused by the implantation of iodoform and calcium hydroxide in the back of rats. These drugs may be used as intracanal dressings to eliminate residual bacteria of the root canal system. Methods: Twenty albinic rats (Rattus norvegicus, var Wistar) were divided into four groups: control group 1 (CG1) had normal skin; control group 2 (CG2) had wounded tissue without drugs; in groups 3 and 4, iodoform (IG) and calcium hydroxide (CHG) were inserted into the wounds, respectively. After 3, 5 and 11 days, slices of the implanted areas were macroscopically and microscopically observed regarding to their qualitative and quantitative aspects.Results: In the macroscopical analysis, the CHG showed a large area of necrosis and swelling, which progressively decreased; in the IG the presence of iodoform surrounded by normal tissue was observed. The qualitative and quantitative histological analysis showed that IG promoted a shorter delay in the inflammatory response than the CHG.Conclusion: The inflammatory reaction for iodoform had a peak period five days after the drug insertion. By comparison, calcium hydroxide showed a very large area of necrosis that could only be partially eliminated after eleven days.Key words: Calcium hydroxide; Endodontics; inflammation; iodoform ResumoObjetivo: O objetivo deste estudo foi avaliar a resposta inflamatória causada pela implantação do iodofórmio ou hidróxido de cálcio em dorso de ratos. Estas drogas podem ser usadas como curativo intracanal para eliminar bactérias residuais do sistema de canal radicular.Metodologia: Foram utilizados 20 ratos albinos (Rattus norvegicus, var Wistar) e divididos em 4 grupos: grupo controle 1 (CG1) representado por tecido normal íntegro; grupo controle 2 (CG2) com ferida e sem medicação; nos grupos 3 e 4, Iodofórmio (IG) e hidróxido de cálcio (CHG) foram, respectivamente, inseridos nas feridas. Após três, cinco e onze dias, cortes microscópicos das áreas implantadas foram observados macroscópica e microscopicamente quanto a seus aspectos qualitativos e quantitativos.Resultados: Na análise macroscópica, o CHG mostrou uma grande área de necrose e edema, o que diminuiu progressivamente, no GI, a presença de iodofórmio rodeada por tecido normal foi observado. A análise qualitativa e quantitativa histológica mostrou que IG promoveu um prazo mais curto na resposta inflamatória do que o CHG.Conclusão: A reação inflamatória de iodofórmio teve um período de pico de cinco dias após a inserção de drogas. Em comparação, o hidróxido de cálcio mostrou uma grande área de necrose que só poderia ser parcialmente eliminada após onze dias.

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Section: • 3 Dayssupporting

confidence: 70%

Section: Methodsmentioning

confidence: 99%

Section: • 3 Daysmentioning

confidence: 99%

See 1 more Smart Citation

Tissue inflammatory response to implantation of calcium hydroxide and iodoform in the back of rats

Pallotta¹,

Machado²,

Reis³

et al. 2010

Rev. odonto ciênc. (Online)

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“…The method is now a common preliminary biocompatibility test, and it is currently used in rats to verify the biocompatibility of implantation material in subcutaneous tissue [25,26]. In this way, researchers can evaluate the tissue response around the implants [33,34], and the test has been widely accepted for this purpose [35]. Polyethylene tubes do not induce persistent irritation, and the tested material remains in the surrounding area.…”

Section: Discussionmentioning

confidence: 99%

Mixing failures of endodontic sealers: an in vivo biocompatibility study

Bueno

Lopes

Valentim

et al. 2017

BDS

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Objective: Evaluate, in vivo, the influence of mixing failures on endodontic sealers. Material and methods: To alveolus analysis, 80 rats were divided into Sealapex® and AH Plus® groups. Within each group, the sealer was subjected to either partial (incomplete homogenization—simulating handling failures) or total mixing (complete homogenization) over two periods of 7 and 30 days (n = 20). The maxillary incisor was extracted and a polyethylene tube containing the sealer was inserted. To quantify edema, 40 male rats were divided into four groups (n = 10). The animals received 2% Evans Blue intravenously, and either AH Plus® or Sealapex® was injected subcutaneously. The rats were euthanized after 3 or 6 hours and analyzed in a spectrophotometer (630 ƞm). To analyze the subcutaneous tissue, 20 rats received polyethylene tube implants with the sealers in the dorsal area (n=10), then euthanized after either 7 or 30 days, and inflammation was evaluated according to an inflammatory cells score. Results: In the alveolar 7-day group, control group presented an inflammation score 1, while all other groups presented a score 2, except AH plus® total mix group (3). After 30 days, all groups presented a score 1. The edemogenic test showed less edema in Sealapex® groups (p < 0.5). In subcutaneous 7-day period, all groups presented score 2. In 30 days, all groups revealed score 1, except AH Plus® partial mix group (2). Conclusion: Regarding mixing of the sealers, there were no significant differences among the groups.KeywordsAH Plus; Inflammation; Materials testing; Root canal filling materials; Sealapex.

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“…One of the most practical and widely used methods is the implantation of the material into the subcutaneous connective tissue of rodents [4][5][6] . The irritative effect of endodontic materials is evaluated by the histopathological examination of the tissue response around the implants [7][8][9][10] .…”

Section: ⅰ Introductionmentioning

confidence: 99%