Biocompatibility of Porous Spherical Calcium Carbonate Microparticles on Hela Cells

Abstract

Recently there has been a wide concern on inorganic nanoparticles as drug delivery carriers. CaCO<sub>3</sub> particles have shown promising potential for the development of carriers for drugs, but little research had been performed regarding their safe dosage for maximizing the therapeutic activity without harming biosystems. In this study, we assessed the biological safety of porous spherical CaCO<sub>3</sub> microparticles on Hela cells. The reactive oxygen species (ROS), glutathione (GSH), carbonyl content in proteins (CCP), DNA-protein crosslinks (DPC) and cell viability were measured. Results showed that with the exposure concentration increase, ROS and CCP in Hela cells presented a significant increase but GSH contents in Hela cells and cell viability showed a significant decrease respectively compared with the control. DPC coefficient ascended, but no statistically significant changes were observed. The results indicated that porous spherical CaCO<sub>3</sub> microparticles may induce oxidative damage to Hela cells. But compared with other nanomaterials, porous spherical CaCO<sub>3</sub> appeared to have good biocompatibility. The results implied that porous spherical calcium carbonate microparticles could be applied as relatively safe drug vehicles, but with the caveat that the effect of high dosages should not be ignored when attempting to maximize therapeutic activity by increasing the concentration

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“…The plate was kept in a dark room for 30 minutes, and optical densities of the solutions were measured at a wavelength of 570 nm by the enzyme-linked immunosorbent assay reader. 9,21,22 The percentage cell viability was calculated and expressed as percentage using the following equation:…”

Double hydrophilic polyphosphoester containing copolymers as efficient templating agents for calcium carbonate microparticles

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