Biocompatibility of PLGA/sP(EO-stat-PO)-Coated Mesh Surfaces under Constant Shearing Stress

Abstract

Background: In order to allow inflammatory response modification and ultimately improvement in tissue remodeling, we developed a new surface modification for meshes that will serve as a carrier for other substances. Biocompatibility is tested in an animal model. Methods: The animal model for diaphragmatic hernia repair was established in prior studies. Meshes were surface modified with star-configured PEO (polyethylene oxide)-based molecules [sP(EO-stat-PO)]. An electrospun nanoweb of short-term absorbable PLGA (polylactide-co-glycolide) with integrated sP(EO-stat-PO) molecules was applied onto the modified meshes. This coating also served as aerial sealing of the diaphragm. A final layer of hydrogel was applied to the product. Adhesive properties, defect size and mesh shrinkage were determined, and histological and immunohistochemical investigations performed after 4 months. Results: The mean defect size decreased markedly in both modified mesh groups. Histologically and with regard to apoptosis and proliferation rate, smooth muscle cells, collagen I/III ratio and macrophage count, no statistically significant difference was seen between the 3 mesh groups. Conclusions: In this proof-of-principle investigation, we demonstrate good biocompatibility for this surface-modified mesh compared to a standard polypropylene-based mesh. This new coating represents a promising tool as a carrier for bioactive substances in the near future.

AcuteIn VivoResponse to an Alternative Implant for Urogynecology

“…While some of these animal studies found higher biocompatibility for the polypropylene light meshes compared to the polypropylene control group [20, 21], others found that the outcomes were very similar between the two groups [22]. Some of these meshes have now been introduced into the market since these are thought to be associated with lower complications.…”

Coating of Mesh Grafts for Prolapse and Urinary Incontinence Repair with Autologous Plasma: Exploration Stage of a Surgical Innovation

“…New developments in material optimization are currently tested. There are only a few groups who have investigated polypropylene mesh modifications by surface coating with collagen, titanium, or absorbable polymers in animal and in vitro studies [ 11 , 12 , 23 , 24 ]. While some of these studies found higher biocompatibility (e.g., light polypropylene mesh) compared to the standard polypropylene control group, others found very similar outcomes between the two groups.…”

Influence of MMP inhibitor GM6001 loading of fibre coated polypropylene meshes on wound healing: Implications for hernia repair

“…As a first approach, we covered standard PP mesh with absorbable electrospun nanofibres resulting in good biocompatibility. 7 In this study, the biointegration of these nanofibre-coated PP meshes was investigated in a rat incisional hernia model. We further modified the mesh by additional incorporation of the broad-spectrum MMP inhibitor GM6001 up to the chemical saturation level of the nanofibres.…”

“…47,48 Results were expressed as ratio area between collagen type I and III. 7 Measurements took place at the interval of mesh fibril and the surrounding tissue.…”

“…The biocompatibility of the meshes showed no difference compared to the standard PP-based meshes. 7 Using such a basic PP mesh one can further modulate the mesh surface according to individual needs. Some effort has been made over many years to cover the mesh surfaces in order to avoid bowel adhesions when placing the mesh intraabdominally.…”