Biocompatibility of microcapsules for cell immobilization elaborated with different type of alginates

Orive, Gorka; Márquez, Sara Ponce; Hernández, Rosa Marı́a; Rodríguez-Gascón, Alicia; Igartua, Manoli; Pedraz, José Luís

doi:10.1016/s0142-9612(02)00118-7

Cited by 265 publications

(179 citation statements)

References 26 publications

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“…Alginate is totally biocompatible and can exist in the form of a hydrogel. Different bivalent cations that bind alginate might be used to obtain the gel, with barium being the most used since it forms stronger gels with reduced permeability due to its high affinity to alginate [108,109]. The ability of alginate to chelate bivalent cations is linked to the G/M ratio.…”

Section: The Encapsulation Chancementioning

confidence: 99%

Employment of Microencapsulated Sertoli Cells as a New Tool to Treat Duchenne Muscular Dystrophy

Chiappalupi

Salvadori

Luca

et al. 2017

JFMK

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Duchenne muscular dystrophy (DMD) is a lethal X-linked pathology due to lack of dystrophin and characterized by progressive muscle degeneration, impaired locomotion and premature death. The chronic presence of inflammatory cells, fibrosis and fat deposition are hallmarks of DMD muscle tissue. Many different therapeutic approaches to DMD have been tested, including cell-based and gene-based approaches, exon skipping, induction of expression of the dystrophin paralogue, utrophin, and, most recently the application of the CASPR/Cas9 genome editing system. However, corticosteroid treatment remains the gold standard therapy, even if corticosteroids have shown multiple undesirable side effects. Sertoli cells (SeC) have long been known for their ability to produce immunomodulatory and trophic factors, and have been used in a plethora of experimental models of disease. Recently, microencapsulated porcine SeC (MC-SeC) injected intraperitoneally in dystrophic mice produced morphological and functional benefits in muscles thanks to their release into the circulation of anti-inflammatory factors and heregulin β1, a known inducer of utrophin expression, thus opening a new avenue in the treatment of DMD. In order to stress the potentiality of the use of MC-SeC in the treatment of DMD, here, we examine the principal therapeutic approaches to DMD, and the properties of SeC (either nude or encapsulated into alginate-based microcapsules) and their preclinical and clinical use. Finally, we discuss the potential and future development of this latter approach.Keywords: Duchenne muscular dystrophy; therapeutic approaches; Sertoli cell; muscle inflammation; myopathies; encapsulation; biomaterials Duchenne Muscular Dystrophy (DMD)Duchenne muscular dystrophy (DMD) is the most common muscular dystrophy. Muscular dystrophies are a group of inherited muscle diseases characterized by mutations in specific genes and resulting in muscle degeneration, impaired locomotion and premature death [1,2]. DMD is an X-linked recessive pathology caused by mutations in the dystrophin gene (DMD) usually resulting in the complete absence of this protein. Dystrophin is an essential component of the dystrophin-associated protein complex (DAPC) at the sarcolemma, a complex that ensures the structural and functional integrity of the myofibers during contraction representing a mechanical link between the intracellular cytoskeleton and the extracellular matrix. Absence of dystrophin or other components of the DAPC compromises the integrity of the DAPC itself leading to a susceptibility of myofibers to degeneration

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Section: The Encapsulation Chancementioning

confidence: 99%

Employment of Microencapsulated Sertoli Cells as a New Tool to Treat Duchenne Muscular Dystrophy

Chiappalupi

Salvadori

Luca

et al. 2017

JFMK

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“…From previous studies, it is known that alginate is a suitable carrier for stem cells, allowing chondrogenesis under appropriate culture conditions (Ghidoni et al, 2008). Apart from this, alginate is already approved for clinical use for various purposes, which would make development of a treatment for cartilage defects involving alginate as a cell carrier a realistic option (Thomas, 2000;Orive et al, 2002;Almqvist et al, 2009;Basta et al, 2011;Rokstad et al, 2011). Fibrin is well known for its applications for regeneration of various tissues, among which are repair strategies for cartilage and bone (Ito et al, 2006;Haleem et al, 2010;Liao et al, 2011;Wu et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Chondrogenic differentiation of human bone marrow-derived mesenchymal stem cells in a simulated osteochondral environment is hydrogel dependent

Melle

Tihaya²,

Kops³

et al. 2014

eCM

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Hydrogels pose interesting features for cartilage regeneration strategies, such as the option for injectability and in situ gelation resulting in optimal filling of defects. We aimed to study different hydrogels for their capability to support chondrogenesis of human bone marrow-derived mesenchymal stem cells (hBMSCs). hBMSCs were encapsulated in alginate, alginate with hyaluronic acid (alginate/HA), fibrin or thermoresponsive HA grafted with poly(N-isopropyl acrylamide) side-chains (HA-pNIPAM). Glycosaminoglycan production and cartilage-related gene expression were significantly higher in hBMSCalginate and hBMSC-fibrin constructs than in the other constructs. Supplementation of alginate with HA was not beneficial. hBMSC-alginate, hBMSC-fibrin and hBMSC-HA-pNIPAM constructs were placed in simulated defects in osteochondral biopsies and cultured in vitro for 28 d. Biopsies containing hBMSC-alginate and hBMSC-fibrin were implanted subcutaneously in nude mice for 12 weeks. hBMSC-alginate constructs had significantly higher cartilage-related gene expression after 28 d of culture as well as significantly more safranin-O positive repair tissue after 12 weeks in vivo than hBMSC-fibrin constructs. Although initial experiments with hBMSC-hydrogel constructs suggested comparable results of hBMSC-alginate, hBMSCfibrin and hBMSC-HA-pNIPAM constructs, culture in the osteochondral biopsy model in vitro as well as in vivo revealed differences, suggests that chondrogenesis of hBMSCs in an osteochondral environment is hydrogeldependent.

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“…In the case of cellular response, some research groups found immunostimulatory activity caused by those alginates with high mannuronic acid content, and immunosuppressive activity caused by alginates with high guluronic acid content. It was concluded that mannuronic acid oligomers would provoke cytokine release by macrophages by a receptor-mediated mechanism, whereas guluronic oligomers should inhibit this reaction (Orive et al, 2002).…”

Section: Alginatementioning

confidence: 99%

Polysaccharide-Based Nanoparticles for Controlled Release Formulations

Martı́nez¹,

Fernandez²,

Pérez³

et al. 2012

The Delivery of Nanoparticles

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Biocompatibility of microcapsules for cell immobilization elaborated with different type of alginates

Cited by 265 publications

References 26 publications

Employment of Microencapsulated Sertoli Cells as a New Tool to Treat Duchenne Muscular Dystrophy

Employment of Microencapsulated Sertoli Cells as a New Tool to Treat Duchenne Muscular Dystrophy

Chondrogenic differentiation of human bone marrow-derived mesenchymal stem cells in a simulated osteochondral environment is hydrogel dependent

Polysaccharide-Based Nanoparticles for Controlled Release Formulations

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