Biocompatibility of mesoporous SBA‐16/hydroxyapatite nanocomposite and dentin demineralized particles on human dental pulp stem cells

Abstract

In the present work, a biomaterial (SBA-16/HA) based on the growth of hydroxyapatite (HA) particles within an organized silica structure SBA-16 (Santa Barbara Amorphous-16) was developed to evaluate its application to act as a porous microenvironment promoting attachment and viability of human dental pulp stem cells of healthy deciduous teeth (SHED). First, SHED were isolated and their phenotypes were evaluated by flow cytometry. The samples of SBA-16/HA were characterized by X-ray diffraction (XRD), small and wide angle X-ray scattering (SWAXS), Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM) equipped with energy dispersive spectra detector (EDS). Afterward, cells were cultured in the eluates of the above-mentioned biomaterial aged for 24 hr, 7. and 14 days. Bio-Oss ® and dentin particles are involved for comparison and cells are cultured in the eluates of these two materials also. Thiazolyl Blue Tetrazolium bromide assay-MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide assay) was used for the determination of cell viability. The results obtained by all aforementioned characterization methods of SBA-16/HA, revealed a uniform spherical mesoporous structure, an intrinsic characteristic of this material. This material displayed excellent biocompatibility on SHEDs, and even proliferative potential, indicating that SBA-16/HA could potentially serve as a suitable substrate for bone regeneration.Contrary to SBA-16/HA, dentin particles showed low cytotoxicity at all time points, compared to control and Bio-Oss ® groups. Our results substantiate the idea that SBA-16/HA has a beneficial effect on SHEDs, thus paving the way toward developing new material for bone replacement.

Enhanced magnetic performance and in‐vitro apatite‐forming ability of the CoFe2O4 doped nano‐hydroxyapatite porous bioceramics

“…Cells proliferated during the 24 h of incubation for both HAP and HAP + 3 vol% CF samples. The proliferation of cells was above 90% for the pure HAP, while the HAP + 3 vol% CF had cell viability above 97% even at the higher concentration (100 μg/mL), what means that the prepared ceramics are excellent biocompatibility (Bulajic et al, 2022; Precnerová et al, 2015). Based on these results, we can conclude that the HAP/ x CF porous ceramics showed no significant antiproliferative effect after 24 h incubation.…”

“…Recently, the development of new types of bioceramics has been highlighted due to their importance in the medical field (Cintra et al, 2022). Among various forms of bioceramics, the hydroxyapatite, Ca 10 (PO4) 6 (OH) 2 or HAP (Bulajic et al, 2022) has more interest from many researchers due to their excellent osteointegrative, good biocompatibility, and excellent osteoconductive performance (Dorozhkin, 2009; Ullah et al, 2022; Wang & Yeung, 2017). HAP has a high ability to interact with proteins because of charged oxygen ions and the positively charged calcium ions (Ca 2+ ) associated with phosphate anions () that interact electrostatically with side groups of proteins, as well as by the chelation of calcium ions of the HAP with electron donor amino acids of proteins (Coutinho et al, 2020; Fiume et al, 2021).…”