Biocompatibility of furcal perforation repair material using cell culture technique: Ketac Molar versus ProRoot MTA

Vajrabhaya, La-ongthong; Korsuwannawong, Suwanna; Jantarat, Jeeraphat; Korre, Sumeth

doi:10.1016/j.tripleo.2006.05.015

Cited by 36 publications

(31 citation statements)

References 15 publications

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“…It is a useful method for evaluating the cytotoxicity of a material, owing to its simplicity, speed, and precision 35) . The assay identifies viable cells after coming in contact with extracts of the test materials.…”

Section: Discussionmentioning

confidence: 99%

Genotoxicity and cytotoxicity of mineral trioxide aggregate and calcium enriched mixture cements on L929 mouse fibroblast cells

et al. 2014

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Mineral trioxide aggregate (MTA) has shown good biocompatibility in several studies. In the present study, the genotoxic and cytotoxic effects of calcium enriched mixture (CEM) were evaluated compared with MTA using MTT and single-cell gel (comet) assays with serial ascending concentrations (0 to 1,000 µg/mL) of tested materials. Cytotoxicity data indicated that there is no significant difference between CEM and MTA at all concentrations except for the full concentration (1,000 µg/mL); CEM had lower cytotoxicity. Genotoxic effects were more evident with CEM at concentrations of 15.6 and 250 µg/mL; however, was less than that of MTA at concentrations of 500 and 1,000 µg/mL. The cytotoxicity and genotoxicity effects of the two experimental groups generally increased with consistency. Under the conditions of this study, CEM is biocompatible in terms of cyto-and genotoxicity. It appears to be an alternative to MTA as an endodontic biomaterial offering several advantages.

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Section: Discussionmentioning

confidence: 99%

Genotoxicity and cytotoxicity of mineral trioxide aggregate and calcium enriched mixture cements on L929 mouse fibroblast cells

et al. 2014

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“…1 Mineral trioxide aggregate (MTA) has been the material of choice for perforation repair since its introduction in the early 1990s. MTA is known to create a biocompatible environment in periodontal tissues and induce cementogenesis when used in a perforated area.…”

Section: Introductionmentioning

confidence: 99%

Comparison of the effects of enamel matrix derivative and mineral trioxide aggregate on the mineralization potential of human cementum-derived cells

Chung

Yang

Kim

et al. 2011

Journal of Dental Sciences

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KEYWORDS enamel matrix derivative; human cementumderived cells; matrix metalloproteinase-13; mineral trioxide aggregate Abstract Background/purpose: Cementogenesis is a critical event in the repair of endodontic perforations and the enamel matrix derivative (EMD) was reported to induce cementogenesis when used in periodontal disease. Yet, there is no study investigating the effects of EMD compared with mineral trioxide aggregate (MTA), a well-known material used for perforation repair. Because cementoblasts play important roles in the repair and regeneration of cementum, the aim of this study was to investigate the effect of EMD on the mineralizing potential of human cementum-derived cells (HCDCs). Materials and methods: HCDCs were cultured as previously described. ProRoot MTA was mixed and placed on the bottom of 6-well tissue culture plate inserts under aseptic conditions. These were allowed to set for 24 hours in a humidified incubator at 37 C. Cells were seeded onto culture plates at an initial density of 5000 cells/cm 2 and were treated with either MTA or EMD. Cells with only culture medium were used as the control. Levels of osteopontin and matrix metalloproteinase-13 were assessed by an enzyme-linked immunosorbent assay with supernatants of the culture media collected on Days 3 and 6 of incubation. Mineralization was detected on Day 6 by Alizarin red staining of cells. Results: Concentrations of osteopontin in the EMD group were significantly higher (P < 0.05) than those in the control group at all time points. On both Days 3 and 6, there was an increase in matrix metalloproteinase-13 expression in the EMD group compared with the control group, but the difference was significant only on Day 6 (P < 0.05).

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“…Root perforation often leads to treatment failure. A nonsurgical approach is recommended as the first choice for the treatment of root perforation 1) . Root perforation repair materials must be biocompatible to minimize adverse effects on periodontal tissues and alveolar bone induced by direct contact 2) .…”

Section: ⅰ Introductionmentioning

confidence: 99%

“…Glass ionomer cements (GIC), since their introduction, have undergone considerable modification in formulation and have been endorsed for use in a number of clinical applications 4) . GIC has been advocated for use as a perforation repair material 1) . The advantage of GIC over other materials is its adhesiveness to dentine, and several studies, in vitro, demonstrate its good sealing ability 1) .…”

Section: ⅰ Introductionmentioning

confidence: 99%

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Comparison of biocompatibility of four root perforation repair materials

Kang

Bae

Koh

et al. 2009

J Korean Acad Conserv Dent

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This study was carried out in order to determine in vitro biocompatibility of white mineral trioxide aggregate (MTA), and to compare it with that of the commonly used materials, i. e. calcium hydroxide liner (Dycal), glass ionomer cement (GIC), and Portland cement which has a similar composition of MTA. To assess the biocompatibility of each material, cytotoxicity was examined using MG-63 cells. The degree of cytotoxicity was evaluated by scanning electron microscopy (SEM) and a colorimetric method, based on reduction of the tetrazolium salt 2,3 bis {2methoxy 4nitro 5[(sulfenylamino) carbonyl] 2H tetrazolium hydroxide} (XTT) assay.The results of SEM revealed the cells in contact with GIC, MTA, and Portland cement at 1 and 3 days were apparently healthy. In contrast, cells in the presence of Dycal appeared rounded and detached. In XTT assay, the cellular activities of the cells incubated with all the test materials except Dycal were similar, which corresponded with the SEM observation. The present study supports the view that MTA is a very biocompatible root perforation repair material. It also suggests that cellular response of Portland cement and GIC are very similar to that of MTA. [J Kor Acad Cons Dent 34(3):192-198, 2009]

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Biocompatibility of furcal perforation repair material using cell culture technique: Ketac Molar versus ProRoot MTA

Cited by 36 publications

References 15 publications

Genotoxicity and cytotoxicity of mineral trioxide aggregate and calcium enriched mixture cements on L929 mouse fibroblast cells

Genotoxicity and cytotoxicity of mineral trioxide aggregate and calcium enriched mixture cements on L929 mouse fibroblast cells

Comparison of the effects of enamel matrix derivative and mineral trioxide aggregate on the mineralization potential of human cementum-derived cells

Comparison of biocompatibility of four root perforation repair materials

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