Biocompatibility of electrospun poly(3-hydroxybutyrate) and its composites scaffoldsfor tissue engineering

Abstract

Development of biodegradable polymers-based scaffolds for tissue engineering is a promising trend in bioengineering. The electrospun scaffolds from poly(3-hydroxybutyrate) (PHB) were produced using different additives that changed the physical and chemical characteristics of the products. As a result, the construct consisting of interwoven threads of different diameter (0.8-3.4 mm) were obtained, the smallest diameter was observed in the threads from the PHB using tetrabutilammonium iodide (TBAI) and titanium oxide II (TiO2) as additives. Mesenchymal stem cells (MSC) were cultivated on the scaffolds for the biocompatibility evaluation of obtained materials. Cells viability was determined by the XTT assay test. It was shown that the scaffold from the interwoven threads of lowest diameter is most favorable for MSC growth in comparison with the polymer film and scaffolds from the threads of larger diameter. Thus, it was shown that the biocompatibility of electrospun PHB scaffolds depended on their microstructure. The obtained data can be used for development of scaffolds for tissue engineering.

Bio-Resorption Control and Biological Response of Magnesium Alloy AZ31 Coated with Poly-β-hydroxybutyrate

“…However, this characteristic may become an intriguing property for biomedical applications, where the in vivo corrosion of the magnesium-based implant, if adequately controlled, involves the formation of a soluble, non-toxic oxide and Mg ions that is harmlessly and easily excreted in the urine. In addition, due to its functional roles and presence in bone tissue, Magnesium may have stimulatory effects on the growth of new bone tissue [27][28][29][30] Thus, it is predictable that magnesium and its alloys could be applied as lightweight, degradable, load bearing orthopedic implants, which would remain present in the body and maintain mechanical integrity over a planned controlled time scale while the bone tissue heals, eventually being replaced by natural tissue [31,32] . The unfortunate complication is that pure magnesium can corrode too quickly in the physiological pH (7.4-7.6) and high chloride environment of the physiological system Polymer PHB was then investigated in our study as a potential Magnesium alloy corrosion-controlling coating.…”

“…Different approaches, such as alloy composition, surface modification, and conversion coatings, have been proposed to improve and to better control the corrosion kinetics of Magnesium alloys [14,[22][23][24][25][26]. Namely, ion implantation [27][28][29], organic coatings [30][31][32][33], alkali heat treatment, micro-arc oxidation [34][35][36], and polymer coating [37][38][39] have been proposed. Among these, biodegradable polymer coatings have been described to have better performance [40][41][42], even if not yet providing proper coating durability and good biocompatibility.…”

“…PHB (Poly-β-hydroxybutyrate) is a natural macromolecule polymer secreted by microorganisms under the condition of unbalanced growth that has been chosen for our study as biodegradable protecting and corrosion rate controlling coating for Magnesium alloy [32].…”

Bioresorption Control and Biological Response of Magnesium Alloy AZ31 Coated with Poly-β-Hydroxybutyrate

“…Different approaches, such as alloy composition, surface modification, and conversion coatings, have been proposed to improve and to better control the corrosion kinetics of magnesium alloys [14,[22][23][24][25][26]. Namely, ion implantation [27][28][29], organic coatings [30][31][32][33], alkali heat treatment, micro-arc oxidation [34][35][36], and polymer coating [37][38][39] have been proposed. Among these, biodegradable polymer coatings have been described to have better performance [40][41][42], even if not yet providing proper coating durability or good biocompatibility.…”

“…PHB (Poly-β-hydroxybutyrate) is a natural macromolecule polymer secreted by microorganisms under the condition of unbalanced growth that was chosen for our study as a biodegradable protecting and corrosion rate-controlling coating for magnesium alloy [32].…”