Biocompatibility of anionic collagen matrix as scaffold for bone healing

Rocha, Lenaldo Branco; Góissis, Gilberto; Rossi, Marcos A.

doi:10.1016/s0142-9612(01)00126-0

Cited by 148 publications

(115 citation statements)

References 28 publications

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“…Highly porous scaffolds are generally used as the substrate for anchorage dependent cells and to facilitate nutrient and metabolite distribution to guide cell growth leading to new bone tissue formation [6]. For bone tissue engineering, biodegradable synthetic polymers such as poly (glycolic acid) (PGA), poly(lactic acid) (PLA), copolymers of poly(DL-lactic-glycolic acid) (PLGA), and biodegradable naturally derived polymers such collagen and fibrin have been studied in this context [7][8][9][10]. Salt-leaching methods are often used to generate the porous foams from these biodegradable polymers [11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Bone tissue engineering with premineralized silk scaffolds

Kim

et al. 2008

Bone

269

244

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Silks fibroin biomaterials are being explored as novel protein-based systems for cell and tissue culture. In the present study, biomimetic growth of calcium phosphate on porous silk fibroin polymeric scaffolds was explored to generate organic/inorganic composites as scaffolds for bone tissue engineering. Aqueous-derived silk fibroin scaffolds were prepared with the addition of polyaspartic acid during processing, followed by the controlled deposition of calcium phosphate by exposure to CaCl 2 and Na 2 HPO 4 . These mineralized protein-composite scaffolds were subsequently seeded with human bone marrow stem cells (hMSC) and cultured in vitro for 6 weeks under osteogenic conditions with or without BMP-2. The extent of osteoconductivity was assessed by cell numbers, alkaline phosphatase and calcium deposition, along with immunohistochemistry for bone related outcomes. The results suggest increased osteoconductive outcomes with an increase in initial content of apatite and BMP-2 in the silk fibroin porous scaffolds. The premineralization of these highly porous silk fibroin protein scaffolds provided enhanced outcomes for the bone tissue engineering.

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Section: Introductionmentioning

confidence: 99%

Bone tissue engineering with premineralized silk scaffolds

Kim

et al. 2008

Bone

269

244

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“…Hydroxyapatite (HA) with the chemical formula of Ca 10 (PO 4 ) 6 (OH) 2 , is the major mineral part of natural bones and teeth. HA is the most commonly used apatite-based biomaterial due to its excellent biocompatibility, osteoconductivity and bioactivity [1,2].…”

Section: Introductionmentioning

confidence: 99%

“…HA is the most commonly used apatite-based biomaterial due to its excellent biocompatibility, osteoconductivity and bioactivity [1,2]. In the past decades, many efforts have been focused on the biomimetic growth of nano-HA through the combination of biodegradable polymers or proteins for bone tissue engineering [3][4][5][6]. Among these polymers, collagen is the best known tissue-derived natural polymer and has been used as tissue-culture scaffolds.…”

Section: Introductionmentioning

confidence: 99%

Effect of polyaspartic acid on hydroxyapatite deposition in silk fibroin blend films

L.¹,

Li²,

Ru³

et al. 2010

Express Polym. Lett.

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Abstract. Polyaspartic acid/silk fibroin/hydroxyapatite (PASP/SF-HA) composites have been synthesized by biomimetic processing. SF solution was mixed with different contents of PASP to prepare the PASP/SF blend membranes. After ethanol treatment and premineralization process, the blend membranes were immersed into 1.5 simulated body fluid (1.5 SBF) for 24 h to induce apatite deposition at 37±0.5°C. Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) results revealed that a conformation transition of SF occurred after the addition of PASP and ethanol treatment. The FTIR and XRD results also confirmed that the main component of apatite deposition was HA. Scanning electron microscopy (SEM) showed that the content of HA increased with increasing PASP concentration .Inductively Coupled Plasma-Atomic Emission Spectrometry (ICP) results revealed that the Ca/P molar ratio could reach 1.45, which was close to the Ca/P ratio of apatite. It was appropriate to conclude that the increasing content of PASP had a distinct effect on HA deposition in the blend films.

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“…2b, c). Chitosan is one of the most promising biopolymers for tissue engineering, and it can potentially be used for bone regeneration applications [19,20,23,34,35]. Gelatin and chondroitin have been employed to improve cell adhesion and activation in 3D cultures [20,34,36,37].…”

Section: Resultsmentioning

confidence: 99%

Healing Pattern in Calvarial Bone Defects Following Bone Regeneration in Rats Guided by Chitosan Scaffold and Adipose Tissue-Derived Mesenchymal Stem Cells

Carvalho¹,

Breyner²,

Hell³

et al. 2012

TOTERMJ

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Cell-based tissue engineering using scaffolds provides a promising option for the repair of bone tissue damage caused by trauma or aging-related degeneration such as osteoporosis. In this study, a porous 3D scaffold was used to support the differentiation process of rat adipose -derived stem cells (ADSCs) into osteoblasts in vitro. The scaffold was made with chitosan, gelatin and chondroitin. In addition, the scaffold was crosslinked by glutaraldehyide. The osteogenic differentiation of ADSCs was improved in 3D culture as shown by the cell viability assay (MTT) and analyses of, alkaline phosphatase activity (ALP), and collagen production during three weeks of culturing. During the 2nd and 3rd weeks of culturing, bone markers, such as osteopontin and osteocalcin, were detected by the PCR analysis. In vivo biocompatibility was evaluated subcutaneously in rats. A mild inflammatory response was observed during the 5 weeks. Reduction of the matrix fibers by reabsorptive cells and formation of new blood vessels were observed during this period. However, no inflammation was observed. Five weeks after the implants were placed in the calvaria -defects, a small amount of bone repair was observed. In addition, immunohistochemistry revealed the presence of EGFPs ADSCs in the newly formed extracellular matrix. These findings indicated that the chitosan-gelatin-chondroitin 3D structure enhances cellular proliferation, which may be useful in the development of biomaterials for the stimulation of adult stem cells in bone tissue engineering.

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Biocompatibility of anionic collagen matrix as scaffold for bone healing

Cited by 148 publications

References 28 publications

Bone tissue engineering with premineralized silk scaffolds

Bone tissue engineering with premineralized silk scaffolds

Effect of polyaspartic acid on hydroxyapatite deposition in silk fibroin blend films

Healing Pattern in Calvarial Bone Defects Following Bone Regeneration in Rats Guided by Chitosan Scaffold and Adipose Tissue-Derived Mesenchymal Stem Cells

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