Biocompatibility of alginate–poly-l-lysine microcapsules for cell therapy

Orive, Gorka; Tam, Susan K.; Pedraz, José Luís; Hallé, Jean‐Pierre

doi:10.1016/j.biomaterials.2006.02.048

Cited by 308 publications

(219 citation statements)

References 62 publications

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“…19,22,23,[29][30][31] In addition to the questionable effect on in vivo biocompatibility, our data demonstrate bead breakage during an osmotic challenge and reduced neurotrophic factor detection associated with PLL coating.…”

mentioning

confidence: 79%

“…27,28 However, the PLL coating layer may itself cause an unfavorable foreign body response and slight toxicity to encapsulated cells, and its use remains controversial. 19,20,22,23,[29][30][31] In light of the extensive research indicating a relationship between alginate composition and encapsulated cell function, as well as the limited amount of data on NSC encapsulation in alginate, the effects of M=G content and PLL coating on entrapped cortical NSCs were investigated. Among the conditions tested, we show that neurotrophic factor release and mechanical stability in response to an osmotic challenge were the most favorable with a high G scaffold without a PLL coating layer.…”

Section: Introductionmentioning

confidence: 99%

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Alginate Composition Effects on a Neural Stem Cell–Seeded Scaffold

Purcell

Singh

Kipke

2009

Tissue Engineering Part C: Methods

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The purpose of this study was to evaluate the effects of alginate composition on the neurotrophic factor release, viability, and proliferation of encapsulated neural stem cells (NSCs), as well as on the mechanical stability of the scaffold itself. Four compositions were tested: a high guluronic acid (68%) and a high mannuronic acid (54%) content alginate, with or without a poly-L-lysine (PLL) coating layer. Enzyme-linked immunosorbent assay was used to quantify the release of brain-derived neurotrophic factor, glial-derived neurotrophic factor, and nerve growth factor from the encapsulated cells. All three factors were detected from encapsulated cells only when a high L-guluronic acid alginate without PLL was used. Additionally, capsules with this composition remained intact more frequently when exposed to solutions of low osmolarity, potentially indicating superior mechanical stability. Alginate beads with a PLL-coated, high D-mannuronic acid composition were the most prone to breakage in the osmotic pressure test, and were too fragile for histology and proliferation assays after 1 week in vitro. NSCs survived and proliferated in the three remaining alginate compositions similarly over the 21-day study course irrespective of scaffold condition. NSC-seeded alginate beads with a high L-guluronic acid, non-PLL-coated composition may be useful in the repair of injured nervous tissue, where the mechanism is the secretion of neuroprotective factors. We verify the neuroprotective effects of medium conditioned by NSCseeded alginate beads on the serum withdrawal-mediated death of PC-12 cells here.

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mentioning

confidence: 79%

Section: Introductionmentioning

confidence: 99%

Alginate Composition Effects on a Neural Stem Cell–Seeded Scaffold

Purcell

Singh

Kipke

2009

Tissue Engineering Part C: Methods

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“…Alginate is an unbranched natural copolymer composed of β-D-mannuronate (M) and α-L-guluronate (G), linked together by 1,4 bonds (Garbayo et al, 2002;Bajpai and Sharma, 2004). Due to its relative stability, biocompatibility, adjustable porosity and simplicity of use, alginate is thus a biomaterial of choice when it comes to entrapping cells (Garbayo et al, 2002;Zmora et al, 2002;David et al, 2004a;De Vos et al, 2006;Zimmermann et al, 2007;Wikstrom et al, 2008), to cell therapy (Chang, 2005;Paul et al, 2009) or to being used in medical devices (Ueyama et al, 2002;Orive et al, 2004;Gao et al, 2005;De Vos et al, 2006;Orive et al, 2006;Wikstrom et al, 2008). Some of these studies were conducted with hepatocytes that were either encapsulated within alginate beads (Selden et al, 1999;David et al, 2004b;Gao et al, 2005;Kinasiewicz et al, 2007;Kinasiewicz et al, 2008) or capsules (Canaple et al, 2001;Orive et al, 2004;Haque et al, 2005), or seeded within alginate scaffolds (Zmora et al, 2002;Seo et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Impact of alginate type and bead diameter on mass transfers and the metabolic activities of encapsulated C3A cells in bioartificial liver applications

Gautier

Carpentier²,

Dufresne³

et al. 2011

eCM

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Liver-assist devices have been developed in the last few decades to support patients with liver failure on the road to recovery or transplantation. Fluidised bed bio-artificial livers -where liver cells are encapsulated within alginate beads -appear to be a valuable alternative to hollow fibre devices for improving mass transfers and enhancing treatment efficacy. This approach nevertheless deserves optimization in terms of bead production. The aim of this study was to investigate the impact of alginate type and of two bead diameters (1000 μm and 600 μm) on mass transfers within beads and on the biological functions of encapsulated C3A cells.After assessing the effect of the encapsulation process on bead quality, we investigated cell viability and metabolic activities (ammonia, albumin, α-foetoprotein synthesis and glucose consumption). They were successfully maintained over 48 h within fluidised bed bioreactors, independently of alginate type and bead diameter. Mass transfers were not significantly influenced by the latter parameters. Finally, suggestions are made for improving the entrapment process as a means of enhancing the treatment efficiency of the fluidised bed bioartificial liver.

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“…A major disadvantage of using small liquid droplets to confine cells is that the droplets will merge with each other unless they are dispersed in an oil phase, which makes it difficult to retrieve cells from the droplets. The hydrogel microcapsule (~250-1000 µm) of natural, biocompatible polymers such as alginate has been widely explored to confine or encapsulate a variety of living cells for transplantation and cell-based therapy (Chang 1996;Maguire et al 2006;Magyar et al 2001;Orive et al 2003;Orive et al 2004;Orive et al 2006;Rohani et al 2008;Torre et al 2007;Wang et al 2006a;Wang et al 2006b). Recently, living cells have been encapsulated in even smaller (~100 µm) microcapsules for better cell survival and transplanation effcacy ).…”

Section: Conventional Vitrificationmentioning

confidence: 99%

Preservation of Embryonic Stem Cells

He¹

2011

Methodological Advances in the Culture, Manipulation and Utilization of Embryonic Stem Cells for Basic and Practical Applicatio

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Biocompatibility of alginate–poly-l-lysine microcapsules for cell therapy

Cited by 308 publications

References 62 publications

Alginate Composition Effects on a Neural Stem Cell–Seeded Scaffold

Alginate Composition Effects on a Neural Stem Cell–Seeded Scaffold

Impact of alginate type and bead diameter on mass transfers and the metabolic activities of encapsulated C3A cells in bioartificial liver applications

Preservation of Embryonic Stem Cells

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