Biocompatibility of a silicone-coated polypropylene hollow fiber oxygenator in an in vitro model

Watanabe, Hiroshi; Hayashi, Jun‐Ichi; Ohzeki, Hajime; Moro, H; Sugawara, Masaaki; Eguchi, Shoji

doi:10.1016/s0003-4975(99)00213-1

Cited by 25 publications

(12 citation statements)

References 15 publications

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“…Currently, a 1–2 m 2 surface area is required to sufficiently transfer CO 2 through the membrane [45,46,47,48]. A surface area of this size lacks the practicalities of functioning effectively within the human body [49,50]. One way that has been effective at increasing the transfer of CO 2 lies in immobilizing CA onto the HFM.…”

Section: Artificial Lungsmentioning

confidence: 99%

Carbonic Anhydrases and Their Biotechnological Applications

et al. 2013

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The carbonic anhydrases (CAs) are mostly zinc-containing metalloenzymes which catalyze the reversible hydration/dehydration of carbon dioxide/bicarbonate. The CAs have been extensively studied because of their broad physiological importance in all kingdoms of life and clinical relevance as drug targets. In particular, human CA isoform II (HCA II) has a catalytic efficiency of 108 M−1 s−1, approaching the diffusion limit. The high catalytic rate, relatively simple procedure of expression and purification, relative stability and extensive biophysical studies of HCA II has made it an exciting candidate to be incorporated into various biomedical applications such as artificial lungs, biosensors and CO2 sequestration systems, among others. This review highlights the current state of these applications, lists their advantages and limitations, and discusses their future development.

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Section: Artificial Lungsmentioning

confidence: 99%

Carbonic Anhydrases and Their Biotechnological Applications

et al. 2013

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“…Baksaas et al found no difference with regard to levels of C3 and C4 between patients operated on using uncoated and bio-passive coated surfaces 13. Watanabe et al reported that there was an increase in C3 levels in both groups in the postoperative period 14. In another study, comparison of phosphorylcholine- and heparin-coated oxygenators demonstrated a rise in C3 levels in both groups 15…”

Section: Discussionmentioning

confidence: 97%

Humoral immune response and coated or uncoated oxygenators during cardiopulmonary bypass surgery

Karakişi

Kunt

Bozok

et al. 2016

CVJA

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SummaryAim:To investigate and compare uncoated and phosphorylcholine-coated oxygenators in terms of induction of humoral immune response during coronary artery bypass surgery.Methods:A total of 20 consecutive patients who underwent coronary artery bypass surgery were randomly distributed into two groups according to the type of oxygenator used during surgery. Group 1 consisted of 10 patients who were operated on using phosphorylcholine-coated oxygenators. Group 2 contained 10 patients who underwent surgery using uncoated oxygenators. Blood and oxygenator fibre samples were obtained and compared in terms of immunoglobulins (IgG, IgM), complements (C3c, C4), serum total protein and albumin levels using electron microscopy and flow cytometry.Results:In group 1, levels of IgM, IgG, total protein and serum albumin were significantly increased at the end of cardiopulmonary bypass (CPB) compared to those at the beginning of CPB. In group 2, C3c and C4 levels at the beginning of CPB were found to be significantly higher than at the end. Electron microscopic examination of oxygenator fibres demonstrated that phosphorylcholine-coated fibres were less likely to be adsorbed by serum proteins and complements than the uncoated fibres.Conclusion:Our results indicate that phosphorylcholine-coated oxygenators seemed to induce humoral immune response to a lesser extent than uncoated oxygenators during coronary artery bypass procedures.

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“…To improve upon the stability of CAs for use in an industrial carbon sequestration setting, researchers have studied the immobilization of CA onto various inorganic (41)(42)(43)(44)(45) and biopolymer surfaces (46,76), which include enriched microorganisms (48,49), in addition to adhesion onto several matrices such as chitosan, alginate, and acrylamide (50,51). The immobilization of CA onto these materials is facilitated by the number of free hydroxyl groups and surface lysine resides of the particular isoform (52) (Figure 16.2).…”

Section: Ca Immobilizationmentioning

confidence: 99%