Biocompatibility of a new cell separator studied by flow cytometry: analyses of platelet antigens during apheresis and storage

Gutensohn, K.; Alisch, A.; Crespeigne, N.; Eifrig, B.; Kuehnl, P.

doi:10.1046/j.1537-2995.1999.39070742.x

Cited by 6 publications

(5 citation statements)

References 33 publications

(42 reference statements)

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“…In the Baxter platelet components, initial platelet activation immediately after apheresis was higher than in the COBE components. In contrast, during storage P‐selectin and CD63 expression increased in the COBE apheresis components, while, similar to earlier observations, the neoantigen expression remained stable in the Baxter PCs 19,24 . The final levels of platelet activation in Baxter and COBE apheresis components were comparable.…”

Section: Discussionsupporting

confidence: 86%

Binding of activated platelets to WBCs in vivo after transfusion

et al. 2002

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Section: Discussionsupporting

confidence: 86%

Binding of activated platelets to WBCs in vivo after transfusion

et al. 2002

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“…Additional sensitive factors are neoantigens such as C62p or CD63 that are secreted from internal granules and exposed on the cell surface (15). The upregulation of this receptor on the platelet surface results in the binding of activated platelets to white cells and their subsequent clearance either by the mononuclear phagocyte system or by entrapment of the platelet white cell conjugates in the microcirculation (3). As a consequence, platelet concentrates with a high proportion of activated platelets may have reduced in vivo efficacy.…”

Section: Discussionmentioning

confidence: 99%

“…One of the most sensitive and specific methods of detecting minimal alterations in platelets and associated microstructures is flow cytometry (2). Antigenic changes at the single cell level can be detected and used for improved quality control and the evaluation of blood–biomedical material interaction (2,3).…”

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confidence: 99%

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Influence of Storage Time on Activation of Platelets Collected with CS 3000 Plus and Cobe Spectra Using Platelet Storage Containers

Kutlay

İlhan

Arslan

et al. 2002

Therapeutic Apheresis

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The aim of this study was to evaluate the in vitro activation in platelet suspensions collected with CS 3000 Plus and Cobe Spectra cell separators using platelet storage containers and the role of white blood cell (WBC) concentration of the suspension in this activation. Seventy‐seven donors were subjected to automated platelet donations with 1 type of equipment (37 with Cobe Spectra and 40 with CS 3000 Plus). Blood samples were obtained immediately after separation and on the third day of storage at 22°C in constant agitation. The WBC concentrations of these samples were studied before storage. Paraformaldehyde‐fixed platelets were incubated with 2 murine monoclonal antibodies: CD42b and CD62. Murine monoclonal antibody immunoglobulin G was used as a negative isotypic control. Bound antibody was then quantitated by flow cytometry. On the third day of storage, a significant increase in CD62 expression rate was observed in platelet suspensions collected with both kinds of equipment. Mean expression rates for Cobe Spectra on Day 0 and Day 3 were 25.6 ± 6.2% and 69.2 ± 9.7%, respectively. Mean expression rates for CS 3000 Plus on Day 0 and Day 3 were 23.4 ± 8.2% and 67.0 ± 8.2%, respectively. The mean results for both devices were 22.8 ± 4.56% for Day 0 and 68.7 ± 13.2% for Day 3. There was no difference between CD42b mean fluorescence intensity on Days 0 and 3 for the 2 devices (p > 0.5). Mean WBC concentrations in the platelet suspensions for Cobe Spectra and CS 3000 Plus were 0.37 × 103/μl and 0.42 × 103/μl, respectively, and there was no relation between WBC concentration and increase in CD62 expression. Both kinds of equipment were found to be similar according to in vitro activation markers.

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“…There are well documented differences between apheresis devices and P‐selectin expression of the harvested platelets [33, 34], whereby the initial activation on day 1 was shown to predict the activation values on day 5 of storage [35]. Further, there is good evidence that P‐selectin increases during storage, while the residual ‘activatability’ to express P‐selectin declines [21, 22, 25, 33–38].…”

Section: In Vitro Methods With No or Low Shearmentioning

confidence: 99%