Biocompatibility of a fish scale-derived artificial cornea: Cytotoxicity, cellular adhesion and phenotype, and in vivo immunogenicity

Essen, T. Huibertus van; Zijl, L. van; Possemiers, Tine; Mulder, Aat A.; Zwart, S.J.; Chou, Chih-Hsing; Lin, Chien-Cheng; Lai, HJ; Luyten, Gregorius P M; Tassignon, Marie-José; Zakaria, Nadia; Ghalbzouri, A. El; Jager, Martine J.

doi:10.1016/j.biomaterials.2015.11.015

Cited by 60 publications

(66 citation statements)

References 38 publications

(50 reference statements)

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“…45 Manufactured to an appropriate thickness, a transparent stromal scaffold derived from the fish scale was evaluated after intrastromal implantation in rabbit corneas, where it was found that the scaffold could be retained in vivo for one year, maintaining transparency without eliciting an immune response. 46 As the fish scales are mechanically robust, they additionally have the potential to be sutured onto the cornea in cases of perforating trauma. This application was tested using a model of full-thickness perforations in minipig corneas, 47 where the fish scale-derived scaffold was successfully used as an emergency patch graft (termed the 'Biocornea'), to temporarily seal the cornea and maintain the integrity of the anterior chamber and eye globe for up to four days (within which time a suitable donor cornea can be sourced in an emergency trauma situation).…”

Section: Fish Scale-derived Corneamentioning

confidence: 99%

Corneal Stromal Regeneration: Current Status and Future Therapeutic Potential

Lagali

2019

Current Eye Research

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The corneal stroma comprises 90% of the corneal thickness and is critical for the cornea's transparency and refractive function necessary for vision. When the corneal stroma is altered by disease, injury, or scarring, however, an irreversible loss of transparency can occur. Corneal stromal pathology is the cause of millions of cases of blindness globally, and although corneal transplantation is the standard therapy, a severe global deficit of donor corneal tissue and eye banking infrastructure exists, and is unable to meet the overwhelming need. An alternative approach is to harness the endogenous regenerative ability of the corneal stroma, which exhibits self-renewal of the collagenous extracellular matrix under appropriate conditions. To mimic endogenous stromal regeneration, however, is a challenge. Unlike the corneal epithelium and endothelium, the corneal stroma is an exquisitely organized extracellular matrix containing stromal cells, proteoglycans and corneal nerves that is difficult to recapitulate in vitro. Nevertheless, much progress has recently been made in developing stromal equivalents, and in this review the most recent approaches to stromal regeneration therapy are described and discussed. Novel approaches for stromal regeneration include human or animal corneal and/or non-corneal tissue that is acellular or is decellularized and/or recellularized, acellular bioengineered stromal scaffolds, tissue adhesives, 3D bioprinting and stromal stem cell therapy. This review highlights the techniques and advances that have achieved first clinical use or are close to translation for eventual therapeutic application in repairing and regenerating the corneal stroma, while the potential of these novel therapies for achieving effective stromal regeneration is discussed. ARTICLE HISTORY

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Section: Fish Scale-derived Corneamentioning

confidence: 99%

Corneal Stromal Regeneration: Current Status and Future Therapeutic Potential

Lagali

2019

Current Eye Research

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“…Graft vascularization has also been observed for other collagenous scaffolds, and in most cases, the neovascularization receded after complete corneal reconstruction. 20,34,35 A certain degree of temporary reversible vascularity can promote corneal reconstruction and will not induce severe inflammation or rejection.…”

Section: Discussionmentioning

confidence: 99%

Development of Transparent Acellular Dermal Matrix as Tissue-Engineered Stroma Substitute for Central Lamellar Keratoplasty

Wang¹,

Jiang

et al. 2020

Invest. Ophthalmol. Vis. Sci.

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PURPOSE. To improve the transparency of the acellular dermal matrix (ADM) and investigate the optical, mechanical and histologic properties and biocompatibility of transparent ADM (TADM) in lamellar keratoplasty. METHODS. A stepwise sectioning strategy was applied to determine the transparency distribution of the ADM, and TADM was fabricated accordingly. Transmittance measurements, uniaxial tension testing, and histologic staining were applied to detect its properties. Lamellar keratoplasty was performed in rabbits with TADM, and postoperative evaluations were conducted including the transmittance of the transplant area and histologic staining. RESULTS. The transmittance of the ADM increased with increasing depth, and TADM was isolated mechanically at the deepest level. There was a significant improvement in the transmittance of the TADM compared with the ADM, and no significant difference in transmittance between dehydrated TADM and cornea was observed. The elastic modulus of TADM was significantly stronger than that of normal cornea (P = 0.004). TADM consisted of dense collagen fibrils, mainly collagen type I, and the collagen fibril diameter and interfibrillar spacing were determined to be larger than corneal stroma. After lamellar keratoplasty in rabbits, the TADM was well integrated with the host cornea, and transparent cornea without neovascularization was observed at 6 months. Re-epithelization was completed at 1 month, and keratocyte repopulation and collagen remodeling were observed in the graft 3 and 6 months after surgery. CONCLUSIONS. This study presents the transparency distribution of the ADM and a method for obtaining TADM, which demonstrates ideal transparency, strong mechanical properties, and satisfactory biocompatibility when applied in lamellar keratoplasty.

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“…This was higher than the threshold for cytotoxicity, which has been set at 70% according to the ISO standard (ISO: 10993-5:2009 [9]). 34 The live/dead viability/cytotoxicity kit provides a twocolor fluorescence in cell viability test. 35 Live cells with intracellular esterase activity could converse the non-fluorescent cell-permeant Calcein AM to fluorescent calcein, which show green fluorescence.…”

Section: Nsc Cytotoxicitymentioning

confidence: 99%

Laminin functionalized biomimetic apatite to regulate the adhesion and proliferation behaviors of neural stem cells

Luo

Ruan

Liu

et al. 2018

IJN

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BackgroundFunctionalizing biomaterial substrates with biological signals shows promise in regulating neural stem cell (NSC) behaviors through mimicking cellular microenvironment. However, diverse methods for immobilizing biological molecules yields promising results but with many problems. Biomimetic apatite is an excellent carrier due to its non-toxicity, good biocompatibility, biodegradability, and favorable affinity to plenty of molecules. Therefore, it may provide a promising alternative in regulating NSC behaviors.MethodsBiomimetic apatite immobilized with the extracellular protein – laminin (LN) was prepared through coprecipitation process in modified Dulbecco’s phosphate-buffered saline (DPBS) containing LN. The amount of coprecipitated LN and their release kinetics were examined. The adhesion and proliferation behaviors of NSC on biomimetic apatite immobilized with LN were investigated.ResultsThe coprecipitation approach provided well retention of LN within biomimetic apatite up to 28 days, and supported the adhesion and proliferation of NSCs without cytotoxicity. For long-term cultivation, NSCs formed neurosphere-like aggregates on non-functionalized biomimetic apatite. A monolayer of proliferated NSCs on biomimetic apatite with coprecipitated LN was observed and even more stable than the positive control of LN coated tissue-culture treated polystyrene (TCP).ConclusionThe simple and reproducible method of coprecipitation suggests that biomimetic apatite is an ideal carrier to functionalize materials with biological molecules for neural-related applications.

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Biocompatibility of a fish scale-derived artificial cornea: Cytotoxicity, cellular adhesion and phenotype, and in vivo immunogenicity

Abstract: The FSCM allows excellent cell growth, is not immunogenic and is well-tolerated in the cornea, and thus meets the basic criteria to serve as a scaffold to reconstitute the cornea.

Cited by 60 publications

References 38 publications

Corneal Stromal Regeneration: Current Status and Future Therapeutic Potential

Corneal Stromal Regeneration: Current Status and Future Therapeutic Potential

Development of Transparent Acellular Dermal Matrix as Tissue-Engineered Stroma Substitute for Central Lamellar Keratoplasty

Laminin functionalized biomimetic apatite to regulate the adhesion and proliferation behaviors of neural stem cells

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