Biocompatibility of 4-META/MMA-TBB resin used as a dental luting agent

Nakagawa, Kaori; Saita, Makiko; Ikeda, Takayuki; Hirota, Makoto; Park, Won-Hee; Lee, Masaichi Chang-il; Ogawa, Takahiro

doi:10.1016/j.prosdent.2014.10.016

Cited by 26 publications

(25 citation statements)

References 38 publications

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“…With respect to minimizing free radical production, we tested tri-n-butyl borane (TBB) as a polymerization initiator. TBB better promotes polymerization than BPO, leaving less residual monomer [35] while suppressing the production of free radicals [36]. In addition, TBB-initiated polymerization generates less heat than BPO [37].…”

Section: Introductionmentioning

confidence: 99%

Novel Osteogenic Behaviors around Hydrophilic and Radical-Free 4-META/MMA-TBB: Implications of an Osseointegrating Bone Cement

Sugita

Okubo

Saita

et al. 2020

IJMS

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Poly(methyl methacrylate) (PMMA)-based bone cement, which is widely used to affix orthopedic metallic implants, is considered bio-tolerant but lacks osteoconductivity and is cytotoxic. Implant loosening and toxic complications are significant and recognized problems. Here we devised two strategies to improve PMMA-based bone cement: (1) adding 4-methacryloyloxylethyl trimellitate anhydride (4-META) to MMA monomer to render it hydrophilic; and (2) using tri-n-butyl borane (TBB) as a polymerization initiator instead of benzoyl peroxide (BPO) to reduce free radical production. Rat bone marrow-derived osteoblasts were cultured on PMMA-BPO, common bone cement ingredients, and 4-META/MMA-TBB, newly formulated ingredients. After 24 h of incubation, more cells survived on 4-META/MMA-TBB than on PMMA-BPO. The mineralized area was 20-times greater on 4-META/MMA-TBB than PMMA-BPO at the later culture stage and was accompanied by upregulated osteogenic gene expression. The strength of bone-to-cement integration in rat femurs was 4- and 7-times greater for 4-META/MMA-TBB than PMMA-BPO during early- and late-stage healing, respectively. MicroCT and histomorphometric analyses revealed contact osteogenesis exclusively around 4-META/MMA-TBB, with minimal soft tissue interposition. Hydrophilicity of 4-META/MMA-TBB was sustained for 24 h, particularly under wet conditions, whereas PMMA-BPO was hydrophobic immediately after mixing and was unaffected by time or condition. Electron spin resonance (ESR) spectroscopy revealed that the free radical production for 4-META/MMA-TBB was 1/10 to 1/20 that of PMMA-BPO within 24 h, and the substantial difference persisted for at least 10 days. The compromised ability of PMMA-BPO in recruiting cells was substantially alleviated by adding free radical-scavenging amino-acid N-acetyl cysteine (NAC) into the material, whereas adding NAC did not affect the ability of 4-META/MMA-TBB. These results suggest that 4-META/MMA-TBB shows significantly reduced cytotoxicity compared to PMMA-BPO and induces osteoconductivity due to uniquely created hydrophilic and radical-free interface. Further pre-clinical and clinical validations are warranted.

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Section: Introductionmentioning

confidence: 99%

Novel Osteogenic Behaviors around Hydrophilic and Radical-Free 4-META/MMA-TBB: Implications of an Osseointegrating Bone Cement

Sugita

Okubo

Saita

et al. 2020

IJMS

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“…The in vitro studies can be conducted under controlled conditions and provide a significant amount of information [38]. Different cell lines are used to probe the biocompatibility of dental materials like pulp cells extracted from the maxillary incisors of Sprague Dawley rats, multipotent stromal cells, human foreskin fibroblast, human lung fibroblast, primary human osteoblast, L929 mouse fibroblast, SV40 large T-antigen-transfected bovine dental pulpderived cells, MDPC-23 odontoblast-like cell line, primary pulp fibroblast, and human alveolar osteoblast, for example [39][40][41][42][43][44][45][46]. In this study, the MA-104 epithelial cell line was used because the glass ionomer cements are in direct contact with the epithelial tissue of the oral mucosa.…”

Section: Discussionmentioning

confidence: 99%

Biocompatibility and Surface Characteristics of Resin-Modified Glass Ionomer Cements with Ammonium Quaternary Compounds or Silver Nanoparticles: AnIn VitroStudy

Munguía-Moreno

Martínez-Castañón

Patiño-Marín

et al. 2018

Journal of Nanomaterials

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Glass ionomer cements are materials with diverse clinical applications. Its use is indicated in patients with special needs, pediatric patients, and the elderly; accordingly, it is important to know its properties. The aim of the present study was to determine the cytotoxicity, surface roughness, microhardness, and surface characteristics of GC Fuji ORTHO LC and GC Fuji PLUS resin-modified glass ionomer cements (RMGICs) with 1 and 2% of benzalkonium chloride, cetylpyridinium chloride, hexadecyltrimethylammonium bromide, or silver nanoparticles. All the experimental groups increase or decrease statistically significantly the VHN (P<0.05) compared with the control group, except for GC Fuji PLUS added with hexadecyltrimethylammonium bromide 1 wt%. In the same way, all groups show a statistically significant (P<0.05) increase or decrease in Ra compared with the control group except for GC Fuji ORTHO added with benzalkonium chloride 2 wt%, GC Fuji PLUS added with benzalkonium chloride 2 wt%, and GC Fuji PLUS added with cetylpyridinium chloride 2 wt%. The SEM micrographs show similar surface images between the control and experimental groups. When a dental material is modified, it is important to reevaluate its biological and mechanical characteristics. In the present study, all the additions modified the cytotoxicity and surface characteristics of RMGICs, by increasing or decreasing these properties.

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“…Four-methacryloxyethyl trimellitate anhydride (4-META) in methyl methacrylate (MMA) initiated by tri-n-butyl borane (TBB) (4-META/MMA-TBB) is a unique MMA-based adhesive resin cement, which has been widely used clinically for a long time [10][11][12] . It has been shown to be a reliable resin cement for bonding to tooth structure 13,14) .…”

Section: Introductionmentioning

confidence: 99%

Early bond strengths of 4-META/MMA-TBB resin cements to CAD/CAM resin composite

et al. 2019

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The purpose of this study was to evaluate the early tensile bond strengths of three different resin cements; Super Bond C&B (conventional and quick type) and ResiCem to a CAD/CAM composite block. A CAD/CAM composite block (Shofu Block HC) was ground and silanized according to the manufacturers' instructions before cementation. A conventional tensile bond strength test (Ø: 4 mm) was performed 10 min, 1 h and 24 h after bonding. Super Bond C&B (quick type) showed the highest bond strength at 24 h. However, results of a three-point bend test showed the mechanical properties were weaker for the Super Bond C&B cements compared with composite cement, Resicem. The 4-META/MMA-TBB resin is a suitable resin cement to bond to a CAD/CAM composite block.

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Biocompatibility of 4-META/MMA-TBB resin used as a dental luting agent

Cited by 26 publications

References 38 publications

Novel Osteogenic Behaviors around Hydrophilic and Radical-Free 4-META/MMA-TBB: Implications of an Osseointegrating Bone Cement

Novel Osteogenic Behaviors around Hydrophilic and Radical-Free 4-META/MMA-TBB: Implications of an Osseointegrating Bone Cement

Biocompatibility and Surface Characteristics of Resin-Modified Glass Ionomer Cements with Ammonium Quaternary Compounds or Silver Nanoparticles: AnIn VitroStudy

Early bond strengths of 4-META/MMA-TBB resin cements to CAD/CAM resin composite

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