Biocompatibility, hemostatic properties, and wound healing evaluation of tilapia skin collagen sponges

Abstract

Dialyzed tilapia skin collagen sponge (DTSCS) and self-assembled tilapia skin collagen sponge (STSCS) were prepared by freeze-drying. The raw components used in the fabrication of DTSCS and STSCS were separated and purified from tilapia fish skin. It is anticipated that these collagen sponges could be developed into medical dressings for hemostasis and wound healing. The aim of the present research was to explore the possibility of DTSCS and STSCS as medical dressings and compare their differences by scanning electron microscopy (SEM), water absorption measurement, differential scanning calorimetry (DSC), measurement of porosity, cytotoxicity, hemolysis, in vivo biocompatibility, and evaluation of hemostatic performance and wound healing. The results indicate that DTSCS and STSCS are suitable materials for use in medical applications with a loose and porous structure, high water absorption, high porosity, and high thermal stability. The materials also displayed good biocompatibility, including excellent blood compatibility, a lack of cytotoxicity, with no apparent rejection following implantation. STSCS exhibited rapid hemostasis and promoted healing, with slightly greater efficacy than DTSCS. The hemostatic properties and promotion of healing in DTSCS was similar to that of commercial bovine collagen sponge. Therefore, DTSCS and STSCS both represented excellent potential candidate materials for use as hemostatic agents and wound dressings.

Engineered collagen polymeric materials create noninflammatory regenerative microenvironments that avoid classical foreign body responses

“…An interesting finding was that Th2 and Treg cell markers and signaling pathways were upregulated, implicating these cells in the host's perception of Oligomer scaffolds as “self” rather than “foreign”. Consistent with conventional collagen-based materials, 36,38,70 the commercial collagen displayed a constructive remodeling phenotype, featuring large numbers of macrophages and FBGCs, a mixed Th1/Th17/Th2/Treg lymphocytic reaction, material resorption via phagocytosis and proteolysis, and fibrous tissue formation and remodeling facilitated by fibroblasts (Fig. 7B).…”

“…4,34 All commercial collagen-based materials, regardless of source material and method of manufacture, engage innate and adaptive immune systems, resulting in a distinct FBR, commonly referenced as constructive remodeling, that features immune-mediated material bioresorption (phagocytosis and proteolytic breakdown) and fibrous tissue formation and remodeling. 35–38 Depending on a number of factors ( e.g. , source material, extent of decellularization, processing and exogenous crosslinking, material configuration, implant location), tissue responses can vary between collagen-based products, affecting macrophage polarization, number and distribution of FBGCs, degradation rates, and tissue response outcomes.…”

Clinical Study of Rh-bFGF Combined With Collagen Sponge in the Treatment of Maxillofacial Deep Ⅱ Degree Burn

“…15 According to the function of collagen in absorbing exudate, converging wound surface, and reducing local inflammatory reaction. 16 The author innovatively applied a collagen sponge to cover deep Ⅱ degree burn wound surface. Collagen sponges can replenish wound collagen, thereby increasing skin elasticity after wound healing.…”

Application of Fish Collagen-Nanochitosan-Henna Extract Composites for the Control of Skin Pathogens and Accelerating Wound Healing

“…The traditional Cg sources, e.g., porcine and bovine Cgs, have currently many problematic concerns for applications in biomedical and cosmetic disciplines, such as religious issues and diseases' transmission risks. Thus, fish Cg introduced splendid alternatives for applications in biomedicine, tissue engineering, skin care, and regeneration, with higher biocompatibility to human and minimum disease transmission risk [7][8][9].…”