Biocompatibility, Biomineralization and Induction of Collagen Maturation with the Use of Calcium Hydroxide and Iodoform Intracanal Dressing

Abstract

Biocompatibility and biomineralization of root canal dressings are important requirements for periapical healing. This study evaluated the inflammatory response, biomineralization and tissue repair by collagen fiber maturation in the subcutaneous tissue of rats. Eighteen Wistar rats (n = 6) received subcutaneous implants: calcium hydroxide + propylene glycol [CH+P], calcium hydroxide + propylene glycol + iodoform [CH+P+I], iodoform + carbowax [I+Cwax] and carbowax [Cwax]. Extra empty tubes were used as a control [C]. After 7, 15 and 30 days, the implants were removed with surrounding tissue for staining of hematoxylin-eosin, Von Kossa, picrosirius red and without staining for analysis under polarized light. Results were analyzed via Kruskal–Wallis followed by Dunn testing for nonparametric data and ANOVA followed by a Tukey post hoc test for parametric data (p < 5%). At 7 days, all groups showed a moderate inflammatory reaction and thick fibrous capsule, except the [Cwax] group, with a severe inflammatory infiltrate (p < 0.05). After 15 days, all groups but control had a decrease in inflammatory response. At 30 days, all groups presented a mild reaction and thin fibrous capsule (p > 0.05). Only groups containing calcium hydroxide were found to be positive using Von Kossa staining and polarized light in all periods. At 7 days, all groups showed a higher proportion of immature fibers. At 15 days, the [CH+P] and [Cwax] groups increased their proportion of mature/immature fibers. At 30 days, only the [CH+P] group presented a significant prevalence of mature collagen fibers (p < 0.05). All groups showed biocompatibility, but only groups containing calcium hydroxide induced biomineralization. The addition of iodoform delayed tissue healing.

Aspergilose – Relato De Caso

“…O modelo animal escolhido foi rato Wistar, previamente utilizado em diversas pesquisas odontológicas, seja em investigações sistêmicas como ratos diabéticos (Bueno et al 2021) e hipertensos (Martins et al 2016) ou locais, como análise subcutânea (Bueno et al 2016, Bueno et al 2018, Valentim et al 2021a, Bueno et al 2023a, Bueno et al 2023b. A metodologia de pulpotomia em molares de ratos utilizada no presente estudo também foi previamente utilizado na literatura (Lima et al, 2011;Minic et al, 2021, Valentim et al 2021b.…”

Antibacterial Activity of Endodontic Gutta-Percha—A Systematic Review

“…Another limitation pertained to the duration of bacterial culture incubation on the material's surface in in vitro conditions, ranging from a minimum of 1 h [30] to a maximum of 21 days [20]. Bacterial growth does not follow a consistent vector over time, leading to different periods of incubation yielding diverse, non-uniform, and non-comparable counts of microorganisms.…”

“…Given that the alkalizing potential of the material used for canal obturation exerts a direct influence on microbiota inactivation and tissue healing, this paper measured pH changes after root canal filling with different materials, satisfying the requirements of this systematic review. Most researchers directed their antibacterial evaluations towards Enterococcus faecalis [1][2][3][4][5][7][8][9][10][11][12][15][16][17][18][20][21][22]25,27,29], as it remains the most frequently isolated bacteria from a root canal [30]. Nevertheless, other bacteria underwent analysis, including Staphylococcus aureus [4,5,8,13,14,22,23,25,26], Escherichia coli [5,13,22,23,26], Pseudomonas aeruginosa [4,5,13,14,22,26], Streptococcus mutans [4,23,25], Fusobacterium nucleatum [18,…”

Investigation on the Microstructural Diversity of a Three-Dimensional Porous Hydroxyapatite/Wollastonite Skeleton via Biomineralization in Simulated Body Fluids

“…Currently, hydroxyapatite (HA) stands out as the most commonly employed biomaterial for fabricating these artificial implant skeletons. Following bone reconstruction surgery involving porous HA skeletons, bodily fluids come into contact with and gradually infiltrate these structures, initiating biomineralization [3][4][5][6]. Apatite nanocrystals are slowly formed after the beginning of biomineralization.…”