Biocompatibility, bioactive potential, porosity, and interface analysis calcium silicate repair cements in a dentin tube model

Inada, Rafaela Nanami Handa; Queiroz, Marcela Borsatto; Lopes, Camila Soares; Silva, Evelin Carine Alves; Torres, Fernanda Ferrari Esteves; Silva, Guilherme Ferreira da; Guerreiro-Tanomaru, Juliane Maria; Cerri, Paulo Sérgio; Tanomaru‐Filho, Mário

doi:10.1007/s00784-023-05002-5

Cited by 3 publications

(3 citation statements)

References 39 publications

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“…15 In addition, micro-CT analyses of dentin tubes filled with BCR implanted into subcutaneous tissues of rats showed a low porosity and interface voids. 6 After immersion in PBS, BCR showed negative solubility values that represented a gain in mass. Torres et al 23 also pointed out a gain in mass for BIO after immersion in PBS and in the same way as shown in our study, also demonstrated that this material remained above the minimum level recommended by ISO 6876.…”

Section: Discussionmentioning

confidence: 99%

“…2,3 Bio-C Repair is not cytotoxic to osteoblastic cells, 4 and promoted the deposition of mineralized nodules and cell migration 5 demonstrating its biocompatibility and bioactive potential. 6 BCR showed low volumetric change after immersion in distilled water, with values lower than 3%, after evaluation by micro computed tomography (micro-CT). 7 Dimensional stability and low solubility are essential properties required for endodontic materials, as they reduce the possibility of bacterial infiltration.…”

Section: Introductionmentioning

confidence: 99%

“…8 According to the guidelines of both the International Organization for Standardization (ISO), 9 and American Dental Association (ANSI/ ADA), 10 the solubility of endodontic cements must not exceed 3.0% of mass loss and the change in Dimension (size) should not exceed values of 1.0% shrinkage or 0.1% expansion. 11 The ISO 6876/2012 solubility test 6 has limitations for the purpose of evaluating calcium silicate-based materials due to the hydrophilic nature of bioceramic cements. 12 As regards dimensional changes, single-direction assessment is limited to demonstrating changes in contraction or expansion.…”

Section: Introductionmentioning

confidence: 99%

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Effect of pH on the solubility and volumetric change of ready-to-use Bio-C Repair bioceramic material

SILVA,

PINTO,

GUERREIRO-TANOMARU

et al. 2024

Braz. oral res.

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Acidic pH can modify the properties of repair cements. In this study, volumetric change and solubility of the ready-to-use bioceramic repair cement Bio-C Repair (BCR, Angelus, Londrina, PR, Brazil) were evaluated after immersion in phosphate-buffered saline (PBS) (pH 7.0) or butyric acid (pH 4.5). Solubility was determined by the difference in initial and final mass using polyethylene tubes measuring 4 mm high and 6.70 mm in internal diameter that were filled with BCR and immersed in 7.5 mL of PBS or butyric acid for 7 days. The volumetric change was established by using bovine dentin tubes measuring 4 mm long with an internal diameter of 1.5 mm. The dentin tubes were filled with BCR at 37°C for 24 hours. Scanning was performed with micro-computed tomography (micro-CT; SkyScan 1176, Bruker, Kontich, Belgium) with a voxel size of 8.74 µm. Then, the specimens were immersed in 1.5 mL of PBS or butyric acid at and 37 °C for 7 days. After this period, a new micro-CT scan was performed. Bio-C Repair showed greater mass loss after immersion in butyric acid when compared with immersion in PBS (p<0.05). Bio-C Repair showed volumetric loss after immersion in butyric acid and increase in volume after immersion in PBS (p<0.05). The acidic pH influenced the solubility and dimensional stability of the Bio-C Repair bioceramic cement, promoting a higher percentage of solubility and decrease in volumetric values.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of pH on the solubility and volumetric change of ready-to-use Bio-C Repair bioceramic material

SILVA,

PINTO,

GUERREIRO-TANOMARU

et al. 2024

Braz. oral res.

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Biocompatibility and bioactive potential of NeoPUTTY calcium silicate‐based cement: An in vivo study in rats

Silva,

Pradelli,

da Silva

et al. 2024

Int Endodontic J

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AimTo evaluate the inflammatory reaction and the ability to induce mineralization activity of a new repair material, NeoPUTTY (NPutty; NuSmile, USA), in comparison with Bio‐C Repair (BC; Angelus, Brazil) and MTA Repair HP (MTA HP; Angelus, Brazil).MethodologyPolyethylene tubes were filled with materials or kept empty (control group, CG) and implanted in subcutaneous tissue of rats for 7, 15, 30, and 60 days (n = 6/group). Capsule thickness, number of inflammatory cells (ICs), fibroblasts, collagen content, and von Kossa analysis were performed. Unstained sections were evaluated under polarized light and by immunohistochemistry for osteocalcin (OCN). Data were submitted to two‐way anova followed by Tukey's test (p ≤ .05), except for OCN. OCN data were submitted to Kruskal–Wallis and Dunn and Friedman post hoc tests followed by the Nemenyi test at a significance level of 5%.ResultsAt 7, 15, and 30 days, thick capsules containing numerous ICs were seen around the materials. At 60 days, a moderate inflammatory reaction was observed for NPutty, BC while MTA HP presented thin capsules with moderate inflammatory cells. In all periods, NPutty specimens contained the highest values of ICs (p < .05). From 7 to 60 days, the number of ICs reduced significantly while an increase in the number of fibroblasts and birefringent collagen content was observed. At 7 and 15 days, no significant difference was observed in the immunoexpression of OCN (p > .05). At 30 and 60 days, NPutty showed the lowest values of OCN (p < .05). At 60 days, a similar immunoexpression was observed for BC and MTA HP (p > .05). In all time intervals, capsules around NPutty, BC, and MTA HP showed von Kossa‐positive and birefringent structures.ConclusionsDespite the greater inflammatory reaction promoted by NeoPutty than BC and MTA HP, the reduction in the thickness of capsules, the increase in the number of fibroblasts, and the reduction in the number of ICs indicate that this bioceramic material is biocompatible Furthermore, NeoPutty presents the ability to induce mineralization activity.

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A new proposal for evaluating of the solubility of bioceramic materials in dentin tubes after immersion in PBS: a laboratory investigation

MENDONÇA,

TAVARES,

SANTOS-JUNIOR

et al. 2023

Rev. odontol. UNESP

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Introduction Repair materials must have low solubility. Phosphate buffered saline (PBS) allows simulating clinical condition and interaction with dentin may be important for the correct evaluate of mass loss of bioceramic cements. Objective To evaluate the effect of distilled water (DW), or PBS immersion on the solubility of Bio-C Repair (BCR, Angelus) or MTA Repair HP (MTAHP, Angelus) using a dentin tube model. Material and method Bovine dentin tubes with a length of 4 mm, an internal diameter of 1.5 mm and walls thickness of approximately 1 mm were made. The specimens were immersed in DW for 24h, then filled with BCR or MTAHP (n = 14) and stored in an oven at 37°C and 95% humidity for 24h. After being weighed on a precision balance to determine the initial mass, the specimens were immersed in DW (pH 6.5) or PBS (pH 7.0) (n = 7) for 28 days. Empty tubes also were used for calculating the mass loss of the dentin (n=4). After this period, the specimens were weighed until stabilization of the final mass occurred (0.001g). The solubility of each material was evaluated. ANOVA and Tukey statistical tests were performed (α=0.05). Result BCR and MTAHP showing gain of mass in DW and mass loss in PBS (p<0.05). Conclusion The immersion solution influenced the solubility of BCR and MTAHP using dentin tube model. The new methodological proposal could be an alternative to ISO standards for testing the solubility of bioceramic cements.

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Biocompatibility, bioactive potential, porosity, and interface analysis calcium silicate repair cements in a dentin tube model

Cited by 3 publications

References 39 publications

Effect of pH on the solubility and volumetric change of ready-to-use Bio-C Repair bioceramic material

Effect of pH on the solubility and volumetric change of ready-to-use Bio-C Repair bioceramic material

Biocompatibility and bioactive potential of NeoPUTTY calcium silicate‐based cement: An in vivo study in rats

A new proposal for evaluating of the solubility of bioceramic materials in dentin tubes after immersion in PBS: a laboratory investigation

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