Biocompatibility and osteogenic properties of porous tantalum

Wang, Qian; Zhang, Hui; Li, Qijia; Ye, Lei; Gan, Hu; Liu, Yingjie; Wang, Hui; Wang, Zhiqiang

doi:10.3892/etm.2015.2208

Cited by 67 publications

(61 citation statements)

References 22 publications

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“…Wang et al prepared porous tantalum by the powder metallurgy technique and implanted into the femoral condyle of rabbits. 40 But this method is difficult to get high open-cell porous metals. In recent years, with the rapid development of 3D printing technology, net-shape porous tantalum has been created with laser engineered net shaping technique and applied for bone defects in rat.…”

Section: Discussionmentioning

confidence: 99%

Tantalum coating of porous carbon scaffold supplemented with autologous bone marrow stromal stem cells for bone regenerationin vitroandin vivo

Wei

Zhao

Wang

et al. 2016

Exp Biol Med (Maywood)

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Porous tantalum metal with low elastic modulus is similar to cancellous bone. Reticulated vitreous carbon (RVC) can provide threedimensional pore structure and serves as the ideal scaffold of tantalum coating. In this study, the biocompatibility of domestic porous tantalum was first successfully tested with bone marrow stromal stem cells (BMSCs) in vitro and for bone tissue repair in vivo. We evaluated cytotoxicity of RVC scaffold and tantalum coating using BMSCs. The morphology, adhesion, and proliferation of BMSCs were observed via laser scanning confocal microscope and scanning electron microscopy. In addition, porous tantalum rods with or without autologous BMSCs were implanted on hind legs in dogs, respectively. The osteogenic potential was observed by hard tissue slice examination. At three weeks and six weeks following implantation, new osteoblasts and new bone were observed at the tantalum-host bone interface and pores. At 12 weeks postporous tantalum with autologous BMSCs implantation, regenerated trabecular equivalent to mature bone was found in the pore of tantalum rods. Our results suggested that domestic porous tantalum had excellent biocompatibility and could promote new bone formation in vivo. Meanwhile, the osteogenesis of porous tantalum associated with autologous BMSCs was more excellent than only tantalum implantation. Future clinical studies are warranted to verify the clinical efficacy of combined implantation of this domestic porous tantalum associated with autologous BMSCs implantation and compare their efficacy with conventional autologous bone grafting carrying blood vessel in patients needing bone repairing.

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Section: Discussionmentioning

confidence: 99%

Tantalum coating of porous carbon scaffold supplemented with autologous bone marrow stromal stem cells for bone regenerationin vitroandin vivo

Wei

Zhao

Wang

et al. 2016

Exp Biol Med (Maywood)

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“… Histological observation of hard tissue in contact with porous tantalum implants at week ( A ) 2; ( B ) 4; ( C ) 8 and ( D ) 12 after implantation (methylene blue staining). Reproduced with permission from [ 95 ]. …”

Section: Figurementioning

confidence: 99%

Metallic Biomaterials: Current Challenges and Opportunities

et al. 2017

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Metallic biomaterials are engineered systems designed to provide internal support to biological tissues and they are being used largely in joint replacements, dental implants, orthopaedic fixations and stents. Higher biomaterial usage is associated with an increased incidence of implant-related complications due to poor implant integration, inflammation, mechanical instability, necrosis and infections, and associated prolonged patient care, pain and loss of function. In this review, we will briefly explore major representatives of metallic biomaterials along with the key existing and emerging strategies for surface and bulk modification used to improve biointegration, mechanical strength and flexibility of biometals, and discuss their compatibility with the concept of 3D printing.

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“…7,8 Porous Ta, also called porous Ta trabecular metal (PTTM), contains a co-adjacent vesicular framework that is similar to cancellous bone with pore sizes ranging from 300 to 600 μm and a porosity of 75%-85%, and its elastic modulus (1.3-10 GPa) is similar to that of natural cortical (12-18 GPa) and cancellous bone (0.1-0.5 GPa). 9 Porous Ta is non-toxic, highly corrosion resistant, biocompatible, and bioactive; 10 it allows both bone ingrowth and ongrowth and promotes osteogenesis to establish osseointegration and osseoincorporation, thus significantly enhancing the initial stability of implants 9,11 and the applicability to osteo-regenerative strategies. 12 Currently, porous Ta has been widely applied in orthopedic applications and oral implants, and studies have shown that it can highly promote the adhesion, proliferation, differentiation, and mineralization of osteoblasts in vitro 3,13,14 and in vivo.…”

Section: Introductionmentioning

confidence: 99%

Involvement of autophagy in tantalum nanoparticle-induced osteoblast proliferation

Kang¹,

Wei²,

Song³

et al. 2017

IJN

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Porous tantalum (Ta) implants are highly corrosion resistant and biocompatible, and they possess significantly better initial stability than that of conventional titanium (Ti) implants. During loading wear, Ta nanoparticles (Ta-NPs) that were deposited on the surface of a porous Ta implant are inevitably released and come into direct contact with peri-implant osteoblasts. The wear debris may influence cell behavior and implant stabilization. However, the interaction of Ta-NPs with osteoblasts has not been clearly investigated. This study aimed to investigate the effect of Ta-NPs on cell proliferation and their underlying mechanism. The Cell Counting Kit-8 (CCK-8) assay was used to measure the cell viability of MC3T3-E1 mouse osteoblasts and showed that Ta-NP treatment could increase cell viability. Then, confocal microscopy, Western blotting, and transmission electron microscopy were used to confirm the autophagy induced by Ta-NPs, and evidence of autophagy induction was observed as positive LC3 puncta, high-LC3-II expression, and autophagic vesicle ultrastructures. The CCK-8 assay revealed that the cell viability was further increased and decreased by the application of an autophagy inducer and inhibitor, respectively. In addition, pre-treatment with autophagy inhibitor 3-methyladenine (3-MA) inhibited the Ta-NP-induced autophagy. These results indicate that the Ta-NPs can promote cell proliferation, that an autophagy inducer can further strengthen this effect and that an autophagy inhibitor can weaken this effect. In conclusion, autophagy was involved in Ta-NP-induced cell proliferation and had a promoting effect.

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Biocompatibility and osteogenic properties of porous tantalum

Cited by 67 publications

References 22 publications

Tantalum coating of porous carbon scaffold supplemented with autologous bone marrow stromal stem cells for bone regenerationin vitroandin vivo

Tantalum coating of porous carbon scaffold supplemented with autologous bone marrow stromal stem cells for bone regenerationin vitroandin vivo

Metallic Biomaterials: Current Challenges and Opportunities

Involvement of autophagy in tantalum nanoparticle-induced osteoblast proliferation

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