Biocompatibility and Osteo-Differentiation Study of Poly(ε-Caprolactone) and β-Tricalcium Phosphate Composite Membranes

Abstract

The Tissue Engineering appears with a modern proposal for the treatment of damages or diseases. The study of materials and methods for tissues and organs regeneration by the patient cells culture had been developed on the last years but still couldn’t be used for all different tissues. In this multidisciplinary research field, the present work joins the biodegradability of poly(ε-caprolactone) (PCL) with the osteoconductive properties of β-tricalcium phosphate (β-TCP) in order to create a composite which acts as a temporary support for cell culture without a second surgery to remove the biomaterial. This work evaluates three membranes types, obtained by casting in chloroform, on the biocompatibility and differentiation on mesenquimal stem cells (hMSC). These analyses showed cell viability with the rezasurin method and the alkaline phosphatase activity (ALP). DMA analyses, MEV and OPM were performed.

Effect of laminated hydroxyapatite/gelatin nanocomposite scaffold structure on osteogenesis using unrestricted somatic stem cells in rat

“…HA ceramic (Ca 10 (PO 4 ) 6 (OH) 2 ) is an attractive material for orthopaedic and dental applications, due to its biocompatibility (Jarcho et al, ; Li et al, ) bioactivity, osteoinductivity (Huang et al, ; Gotz et al, ) and non‐immunogenicity (Krause et al, ). Crystal resorption by osteoblast could be more homogenous in case of nHA as crystal bonding is weaker in comparison with micro‐size HA (Sanosh and Chu, ), therefore it promotes osteoblast adhesion, differentiation and proliferation within a short period of time, and therefore can be considered as an ideal scaffold material (Krause et al, ; Hu et al, ; Salgado et al, ). Despite of high biocompatibility and osteoconductivity of nHA (Rezwan and Chen, ), its application in load‐bearing sites is very limited due to low mechanical strength and therefore a nHA/polymer composite scaffold with improved mechanical properties can be suggested to serve as a better substrate for cell attachment in bone tissue engineering (Bose et al, ).…”

Study on polycaprolactone/hydroxylapatite gradient scaffolds for tissue engineering

“…The PCL degradation involves simple mechanisms organized into two stages: random hydrolytic ester cleavage and weight loss through the diffusion of oligomeric species from the bulk. It is a polymer with a very slow degradation rate, depending on the molecular weight [3].…”