Biocompatibility and function of microencapsulated pancreatic islets

Figliuzzi, Marina; Plati, Tiziana; Cornolti, Roberta; Adobati, Federica; Fagiani, Arianna; Rossi, Lisa; Remuzzi, Giuseppe; Remuzzi, Andrea

doi:10.1016/j.actbio.2005.12.002

Cited by 46 publications

(28 citation statements)

References 30 publications

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“…For larger molecules such as albumin and AFP, transfers appears to be hindered by the porous structure, but still take place, indicating that the bead cut off is greater than the size of albumin (66,000 Da). Previous studies have already shown that the bead structure was impermeable to immunoglobulins (Fremond et al, 1996;Clement et al, 1998;Figliuzzi et al, 2006). These results therefore completely meet the requirements for future BAL applications.…”

Section: Discussionsupporting

confidence: 68%

Impact of alginate type and bead diameter on mass transfers and the metabolic activities of encapsulated C3A cells in bioartificial liver applications

Gautier

Carpentier²,

Dufresne³

et al. 2011

eCM

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Liver-assist devices have been developed in the last few decades to support patients with liver failure on the road to recovery or transplantation. Fluidised bed bio-artificial livers -where liver cells are encapsulated within alginate beads -appear to be a valuable alternative to hollow fibre devices for improving mass transfers and enhancing treatment efficacy. This approach nevertheless deserves optimization in terms of bead production. The aim of this study was to investigate the impact of alginate type and of two bead diameters (1000 μm and 600 μm) on mass transfers within beads and on the biological functions of encapsulated C3A cells.After assessing the effect of the encapsulation process on bead quality, we investigated cell viability and metabolic activities (ammonia, albumin, α-foetoprotein synthesis and glucose consumption). They were successfully maintained over 48 h within fluidised bed bioreactors, independently of alginate type and bead diameter. Mass transfers were not significantly influenced by the latter parameters. Finally, suggestions are made for improving the entrapment process as a means of enhancing the treatment efficiency of the fluidised bed bioartificial liver.

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Section: Discussionsupporting

confidence: 68%

Impact of alginate type and bead diameter on mass transfers and the metabolic activities of encapsulated C3A cells in bioartificial liver applications

Gautier

Carpentier²,

Dufresne³

et al. 2011

eCM

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“…Encapsulation and transplantation of pancreatic islets Islet encapsulation was performed as previously described [25]. Briefly, islets were suspended in 1.7% (wt/vol.)…”

Section: Methodsmentioning

confidence: 99%

Regression of diabetic complications by islet transplantation in the rat

et al. 2009

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Aims/hypothesis Type 1 diabetes is a chronic disease leading to complications such as peripheral neuropathies, nephropathy and cardiovascular disease. Pancreatic islet transplantation is being extensively investigated for blood glucose control in animals and in human type 1 diabetic patients, but the question of whether it can reverse long-term diabetic complications has not been fully explored. We investigated the effects of islet transplantation on diabetic complications in a rat model of streptozotocin-induced diabetes.Methods Three groups of rats were used: healthy controls, diabetic and diabetic rats transplanted with microencapsulated islets at 2 months after diabetes induction, when neuropathy was detectable by a decrease in tail nerve conduction velocity (NCV) and impaired nociceptive thresholds. Blood glucose levels and body weight were measured weekly. The variables considered were: thermal (hot plate test) and mechanical sensitivity (Randal-Selitto paw withdrawal test), NCV and Na+, K+-ATPase activity in the sciatic nerve. At the end of the experiments hearts were removed for morphometric determination and myocyte number, and kidneys removed for histological examination. Results Islet transplantation in diabetic rats induced normoglycaemia in a few days, accompanied by a rapid rise in body weight and amelioration of impaired nociceptive thresholds, as well as normalisation of NCV and Na + , K + -ATPase, which were both about 25% below normal in diabetic rats. Myocyte loss was reduced (−34%) by islet transplantation and the observed mild kidney damage of diabetic rats was prevented. Conclusions/interpretation Besides controlling glycaemia, transplantation of microencapsulated pancreatic islets induced almost complete regression of neuropathy and prevented cardiovascular alterations.

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“…Шматочки тканини КНЗ людини перено-сили в 1%-й розчин альгінату ("Fluka", Нор-вегія), який безпосередньо перед застосуван-ням стерилізували, пропускаючи через фільтр з порами 0,45 мкм ("Filtron", Німеччина), після чого здійснювали мікроінкапсуляцію тканини за стандартним методом [10]. Для цього через перший канал генератора мікро-капсул пропускали 1,0%-й розчин альгінату з рівномірно розподіленими шматочками адренокортикальної тканини; через другий канал -повітря зі швидкістю 8-10 л/хв.…”

Section: методикаunclassified