Biocompatibility and Favorable Response of Mesenchymal Stem Cells on Fibronectin-Gold Nanocomposites

Abstract

A simple surface modification method, comprising of a thin coating with gold nanoparticles (AuNPs) and fibronectin (FN), was developed to improve the biocompatibility required for cardiovascular devices. The nanocomposites from FN and AuNPs (FN-Au) were characterized by the atomic force microscopy (AFM), UV-Vis spectrophotometry (UV-Vis), and Fourier transform infrared spectroscopy (FTIR). The biocompatibility of the nanocomposites was evaluated by the response of monocytes and platelets to the material surface in vitro. FN-Au coated surfaces demonstrated low monocyte activation and platelet activation. The behavior of human umbilical cord-derived mesenchymal stem cells (MSCs) on FN-Au was further investigated. MSCs on FN-Au nanocomposites particularly that containing 43.5 ppm of AuNPs (FN-Au 43.5 ppm) showed cell proliferation, low ROS generation, as well as increases in the protein expression levels of matrix metalloproteinase-9 (MMP-9) and endothelial nitric oxide synthase (eNOS), which may account for the enhanced MSC migration on the nanocomposites. These results suggest that the FN-Au nanocomposite thin film coating may serve as a potential and simple solution for the surface modification of blood-contacting devices such as vascular grafts.

Inhibition of colon cancer cell growth by nanoemulsion carrying gold nanoparticles and lycopene

“…Additionally, AN (15 nm) was found to be efficient in inhibiting HePG2-CM-induced proliferation and migration of epithelial cells through the downregulation of vascular endothelial growth factor (VEGF) after incubation of human umbilical vein endothelial cells in HePG2-CM medium. 40 In the present study, we demonstrated that the AN-LP nanoemulsion relative to the LP only treatment exhibited a higher suppressive effect in the migration of the EMT markers of HT-29 cells, as evident by the enhanced expression of E-cadherin and the decreased expressions of Akt and NFkB. Nonetheless, the detailed mechanism of the AN-LP nanoemulsion in inhibiting the migration of cancer cells needs to be further explored.…”

“…In addition, LP was shown to be effective in inhibiting tumor growth and progression in a mouse xenograft model of colon cancer, with the inhibition being inversely proportional to the MMP-9 concentration in mouse blood. 39 The response of mesenchymal stem cells toward fibronectin-gold nanocomposites was studied by Hung et al, 40 illustrating that this treatment could enhance cell proliferation, decrease reactive oxygen species production, and increase expressions of MMP-9 and endothelial nitric oxide synthase which, in turn, affected the migration of human umbilical mesenchymal stem cells. Additionally, AN (15 nm) was found to be efficient in inhibiting HePG2-CM-induced proliferation and migration of epithelial cells through the downregulation of vascular endothelial growth factor (VEGF) after incubation of human umbilical vein endothelial cells in HePG2-CM medium.…”

Homogenized Porcine Extracellular Matrix Derived Injectable Tissue Construct with Gold Nanoparticles for Musculoskeletal Tissue Engineering Applications

“…Reduction in cellular ROS due to AuNP has been shown to be concentration dependent [22]. Similar to the effects of proliferation and migration, the concentration and size of the nanoparticle affects how the cells interact with the particles [39]. Previous studies of cellular ROS concentrations on collagen-100 nm AuNP constructs have shown a concentration dependent decrease in ROS production [16].…”

Real-time visualization of cardiac cell beating behaviour on polymer diffraction gratings

“…42 Hung et al investigated the biocompatibility of bronectin coated gold nanoparticles (FNAuNP) with mesenchymal stem cells. 43 They found the biocompatibility of the FN-AuNP's compared favorably to pure bronectin. Fendyur and Spira were able to culture rat derived cardiomyocytes on laminin coated gold electrodes.…”