Biocompatibility and Efficacy of Collagen/Gelatin Sponge Scaffold With Sustained Release of Basic Fibroblast Growth Factor on Vocal Fold Fibroblasts in 3-Dimensional Culture

Hiwatashi, Nao; Hirano, Shigeru; Mizuta, Masanobu; Tateya, Ichiro; Kanemaru, Shin‐ichi; Nakamura, Tatsuo; Ito, Juichi; Kawai, Katsuya; Suzuki, Shigehiko

doi:10.1177/0003489414546396

Cited by 24 publications

(30 citation statements)

References 35 publications

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“…The collagen/gelatin sponge was used as a drug delivery system of bFGF for the treatment of scarred vocal fold, and had no cytotoxic effects on vocal fold fibroblasts 38,39) . Fluvastatin released from poly(ethylene glycol)-based hydrogels triggered the osteogenic differentiation and modulated the function of hMSCs 40) .…”

Section: Discussionmentioning

confidence: 99%

Influence of lyophilization factors and gelatin concentration on pore structures of atelocollagen/gelatin sponge biomaterial

et al. 2017

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This study aimed to investigate influences of lyophilization factors and gelatin concentration on pore structures of ACG sponge. ACG sponges of different freezing temperatures (−30, −80 and −196 o C), freezing times (1, 2 and 24 h), gelatin concentrations (0.6%AC+0.15%G, 0.6%AC+0.6%G and 0.6%AC+2.4%G), and with 500 μM fluvastatin were fabricated. Pore structures including porosity and pore size were analyzed by scanning electron microscopy and ImageJ. The cytotoxic effects of ACG sponges were evaluated in vitro. Freezing temperature did not affect porosity while high freezing temperature (−30 o C) increased pore size. The high gelatin concentration group (0.6%AC+2.4%G) had decreased porosity and pore size. Freezing time and 500 μM fluvastatin did not affect pore structures. The cytotoxicity and cell proliferation assays revealed that ACG sponges had no cytotoxic effects on human mesenchymal stromal cell growth and proliferation. These results indicate that ACG sponge may be a good biomaterial scaffold for bone regeneration.

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Section: Discussionmentioning

confidence: 99%

Influence of lyophilization factors and gelatin concentration on pore structures of atelocollagen/gelatin sponge biomaterial

et al. 2017

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“…While simple and partially effective, cytokine injections are limited by rapid in vivo absorption and potential need for multiple dosing, potentially causing secondary injuries and further exacerbating scar formation . To prolong cytokine retention, different biomaterial‐based loading systems have been developed, successfully extending release up to 1 month in vivo . However, these loading systems are still incapable of completely restoring vocal fold function, partly attributed to the following: (1) unknown desired cytokine dose and unclear underlying biological mechanism(s) and (2) necessity for repeated surgeries in most cases .…”

Section: Introductionmentioning

confidence: 99%

In vitro evaluation of anti‐fibrotic effects of select cytokines for vocal fold scar treatment

et al. 2018

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Scarring of the vocal fold lamina propria (LP) can cause considerable voice disorders due to reduced pliability in scar tissue, attributed in part to abnormal extracellular matrix (ECM) deposition produced by the fibrotic vocal fold fibroblast (fVFF). Cytokines with anti-fibrotic potential have been investigated to limit abnormal LP ECM, but are limited by the need for repeat injections. Moreover, the potentially significant role played by activated macrophages (AMOs) is usually not considered even though the interaction between AMO and fibrotic fibroblasts is known to regulate scar formation across different tissues. AMO are also regulated by cytokines that are used for LP scar removal, but little is known about AMO behaviors in response to these cytokines within the context of LP scar. In the present study, we evaluated anti-fibrotic effects of hepatocyte growth factor (HGF), interleukin-10 (IL-10) and interleukin-6 (IL-6) in a 3D, in vitro fVFF-AMO co-culture system using poly(ethylene glycol) diacrylate (PEGDA) hydrogels. Data from all cytokines was synthesized into a heat-map that enabled assessment of specific associations between AMO and fVFF phenotypes. Cumulatively, our results indicated that both HGF and IL-10 are potentially anti-fibrotic (reduction in fibrotic markers and enhancement in normal, anti-fibrotic VFF markers), while IL-6 displays more complex, marker specific effects. Possible associations between AMO and fVFF phenotypes were found and may highlight a potential desirable macrophage phenotype. These data support the therapeutic potential of HGF and IL-10 for LP scar treatment, and shed light on future strategies aimed at targeting specific AMO phenotypes. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res B Part B: Appl Biomater, 2018.

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“…Gelatin is a denatured form of collagen that retains its biocompatibility and offers a less expensive alternative to collagen . Gelatin sponges have been used in a variety of tissue engineering applications . In this study, collagen and LM‐111 were chosen to impart bioactivity, and gelatin was used to provide mechanical durability to the biomimetic sponge.…”

Section: Introductionmentioning

confidence: 99%

Biomimetic sponges for regeneration of skeletal muscle following trauma

Haas

Dunn

Marcinczyk

et al. 2018

J Biomedical Materials Res

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Skeletal muscle is inept in regenerating after traumatic injuries due to significant loss of basal lamina and the resident satellite cells. To improve regeneration of skeletal muscle, we have developed biomimetic sponges composed of collagen, gelatin, and laminin (LM)-111 that were crosslinked with 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC). Collagen and LM-111 are crucial components of the muscle extracellular matrix and were chosen to impart bioactivity whereas gelatin and EDC were used to provide mechanical strength to the scaffold. Morphological and mechanical evaluation of the sponges showed porous structure, water-retention capacity and a compressive modulus of 590-808 kPa. The biomimetic sponges supported the infiltration and viability of C 2 C 12 myoblasts over 5 days of culture. The myoblasts produced higher levels of myokines such as VEGF, IL-6, and IGF-1 and showed higher expression of myogenic markers such as MyoD and myogenin on the biomimetic sponges. Biomimetic sponges implanted in a mouse model of volumetric muscle loss (VML) supported satellite, endothelial, and inflammatory cell infiltration but resulted in limited myofiber regeneration at 2 weeks post-injury.

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Biocompatibility and Efficacy of Collagen/Gelatin Sponge Scaffold With Sustained Release of Basic Fibroblast Growth Factor on Vocal Fold Fibroblasts in 3-Dimensional Culture

Abstract: The collagen/gelatin sponge is biocompatible with vocal fold fibroblasts and may be useful as a bFGF drug delivery system for the treatment of scarred vocal folds.

Cited by 24 publications

References 35 publications

Influence of lyophilization factors and gelatin concentration on pore structures of atelocollagen/gelatin sponge biomaterial

Influence of lyophilization factors and gelatin concentration on pore structures of atelocollagen/gelatin sponge biomaterial

In vitro evaluation of anti‐fibrotic effects of select cytokines for vocal fold scar treatment

Biomimetic sponges for regeneration of skeletal muscle following trauma

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