Biocompatibility and Cytotoxic Evaluation of New Sorbent Cartridges for Blood Hemoperfusion

Abstract

Background/Aims: The use of adsorption cartridges for hemoperfusion (HP) is rapidly evolving. For these devices, the potential induced cytotoxicity is an important issue. The aim of this study was to investigate potential in vitro cytotoxic effects of different sorbent cartridges, HA130, HA230, HA330, HA380 (Jafron, China), on U937 monocytes. Methods: Monocytes were exposed to the sorbent material in static and dynamic manners. In static test, cell medium samples were collected after 24 h of incubation in the cartridges. In dynamic test, HP modality has been carried out and samples at 30, 60, 90, and 120 min were collected. Results: Compared to control samples, there was no evidence of increased necrosis or apoptosis in monocytes exposed to the cartridges both in the static and dynamic tests. Conclusion: Our in vitro testing suggests that HA cartridges carry an optimal level of biocompatibility and their use in HP is not associated with adverse reactions or signs of cytotoxicity.

Fluid Dynamics Analysis by CT Imaging Technique of New Sorbent Cartridges for Extracorporeal Therapies

“…A previous study carried out by our research group has demonstrated the biocompatibility, in terms of cytotoxicity, of the housing and the beads material [13]. HA cartridges are among the widely studied and used in China: several trials have been conducted and confirmed their reliability in clinical application [14][15][16], as reported in a recent review [12].…”

“…A recent study published by our group has demonstrated their biocompatibility in terms of cytotoxicity [13].…”

A New Series of Sorbent Devices for Multiple Clinical Purposes: Current Evidence and Future Directions

“…In comparison to control samples, both static and dynamic tests showed no difference in viability, necrosis or apoptosis of monocytes in the samples exposed to HA cartridges. The findings were suggestive that HA cartridges use is not associated with cytotoxicity [5]. With the improvement of adsorption cartridges over the recent years, their use has become adopted more widely.…”

“…The different resin pore size of the sorbent material allows the removal of a wide spectrum of molecular weights. The sorbent devices have the ability to remove endogenous and exogenous materials such as middle uremic toxins, protein bound uremic toxins, hydrophobic or protein bound exogenous substances, cytokines, complements, free hemoglobin, and residual drugs by means of adsorption [5]. HA130 is mainly used in chronic conditions, HA230 in intoxication, and HA330 in acute inflammatory conditions.…”

“…This technique can be really useful for the evaluation of the potential toxicity of materials and can provide an excellent screening tool prior to performing in vivo trials. In a recent in vitro study by Pomarè Montin et al [5], the effect of hemoadsorption (HA) cartridges on cultured monocytes cell line was tested both in static and dynamic manners using a dedicated extracorporeal adsorption circuit. In comparison to control samples, both static and dynamic tests showed no difference in viability, necrosis or apoptosis of monocytes in the samples exposed to HA cartridges.…”