Bioclogging in porous media: influence in reduction of hydraulic conductivity and organic contaminants during synthetic leachate permeation

Abstract

In this study the concept of biofilm accumulation in the sand column was promoted to assess the changes in hydraulic conductivity and concentration of organic contaminants of the synthetic leachate. Four different combinations of column study were carried out using synthetic leachate as a substrate solution. Mixed and stratified mode of experiments with two different sizes (0.3 mm and 0.6 mm) of sand grains were used for column filling. Two columns were acting as a blank, the remaining two columns amended with mixed microbial cultures which were isolated from leachate. The column was operated with continuous synthetic leachate supply for 45 days. The results indicated that the highest hydraulic conductivity reduction occurred in the mixed sand microbial column with 98.8% when compared to stratified sand microbial column. The analysis of organic contaminants of the effluent leachate was also clearly shown that the mixed sand amended with microbes poses a suitable remedial measure when compared to natural and synthetic liners for controlling the leachate migration in the subsurface environment.

In‐situ microbially induced Ca2+‐alginate polymeric sealant for seepage control in porous materials

“…The final value of 4.47 × 10 −7 m s −1 was still 10 times lower than that of the sand column clogged by bacteria augmentation and biofilm formation after a long‐term treatment (e.g. months) (Kanmani et al ., ). The alginate treated sand with low hydraulic conductivity can be used as biopolymer barrier for temporary waterproof engineering applications.…”

Experimental Study on Bioclogging in Porous Media during the Radioactive Effluent Percolation

“…Solution. According to the related literature [8,9], the inoculated volume of Aspergillus niger spore suspension was 1% of water content of the saturated sand which was 30 mL per 10 cm 3 . ere are about 3,400 cm 3 sands in the sand columns, so the inoculated volume of Aspergillus niger spore suspension was about 100 mL.…”

“…Due to the particularity of the radioactive effluent, traditional technologies encounter some obstacles such as the high cost, difficult to find the sources of leakage, low efficiency, and so on [6]. e bioclogging technology has the advantages of detecting and sealing the sources of leakage automatically by inoculating the microorganism and nutrient into porous media, which can reduce the permeability of porous medium and adsorb pollutants by growth, reproduction, and death of microorganisms [7][8][9][10][11][12]. And it is suitable for complex environments.…”

Assessment of CO2 Injection in Fractured Calcite Rock Clogged by Pseudomonas putida Biofilm

“…Biofilms are also used as barriers in porous media and landfills [20,21], in bioremediation enhancement [22,23], prevention of well bore CO 2 leakage [24], and geologic containment CO 2 leakage [25][26][27].…”