Biocleavable comb-shaped gene carriers from dextran backbones with bioreducible ATRP initiation sites

Wang, Zenghui; Zhu, Yun; Chai, M. Y.; Yang, Wantai; Xu, Fu‐Jian

doi:10.1016/j.biomaterials.2011.11.027

Cited by 78 publications

(97 citation statements)

References 23 publications

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“…The percentages of the EGFP-positive HepG2 cells were 28% for POSS-(SS-PDMAEMA3.8K) 8 /pEGFP and 14% for POSS-(PDMAEMA) 8 /pEGFP, and the percentages of the EGFP-positive COS7 cells were 36% for POSS-(SS-PDMAEMA3.8K) 8 /pEGFP and 25% for POSS-(PDMAEMA) 8 / pEGFP respectively, determined by flow cytometry. 10 The above results also confirmed that POSS-(SS-PDMAEMA) 8 with disulfide bridges had much higher transfection activity than POSS-(PDMAEMA) 8 without disulfide linkages.…”

Section: ■ Results and Discussionsupporting

confidence: 89%

Bioreducible POSS-Cored Star-Shaped Polycation for Efficient Gene Delivery

Yang

Wang

et al. 2013

ACS Appl. Mater. Interfaces

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104

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The bioreducible star-shaped gene vector (POSS-(SS-PDMAEMA)8) with well-defined structure and relatively narrow molecular weight distribution was synthesized via atom transfer radical polymerization (ATRP) of (2-dimethylamino)ethyl methacrylate (DMAEMA) from a polyhedral oligomeric silsesquioxane (POSS) macroinitiator. POSS-(SS-PDMAEMA)8 was composed of a biocompatible POSS core and eight disulfide-linked PDMAEMA arms, wherein the PDMAEMA chain length could be adjusted by controlling polymerization time. POSS-(SS-PDMAEMA)8 can effectively bind pDNA into uniform nanocomplexes with appropriate particle size and zeta potential. The incorporation of disulfide bridges gave the POSS-(SS-PDMAEMA)8 material facile bioreducibility. In comparison with POSS-(PDMAEMA)8 without disulfide linkage, POSS-(SS-PDMAEMA)8 exhibited much lower cytotoxicity and substantially higher transfection efficiency. The present work would provide useful information for the design of new POSS-based drug/gene carriers.

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Section: ■ Results and Discussionsupporting

confidence: 89%

Bioreducible POSS-Cored Star-Shaped Polycation for Efficient Gene Delivery

Yang

Wang

et al. 2013

ACS Appl. Mater. Interfaces

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104

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“…Several drug‐delivery systems based on nanocellulose materials for various pharmaceutical applications have been used in recent years . In one study, Trovatti et al .…”

Section: Advanced Nanocellulose Materials For Biomedical Applicationsmentioning

confidence: 99%

Recent advances in nanocellulose for biomedical applications

Jorfi

Foster

2014

J of Applied Polymer Sci

641

368

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Nanocellulose materials have undergone rapid development in recent years as promising biomedical materials because of their excellent physical and biological properties, in particular their biocompatibility, biodegradability, and low cytotoxicity. Recently, a significant amount of research has been directed toward the fabrication of advanced cellulose nanofibers with different morphologies and functional properties. These nanocellulose fibers are widely applied in medical implants, tissue engineering, drug delivery, wound-healing, cardiovascular applications, and other medical applications. In this review, we reflect on recent advancements in the design and fabrication of advanced nanocellulose-based biomaterials (cellulose nanocrystals, bacterial nanocellulose, and cellulose nanofibrils) that are promising for biomedical applications and discuss material requirements for each application, along with the challenges that the materials might face. Finally, we give an overview on future directions of nanocellulose-based materials in the biomedical field.

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“…4. 107 Beside PDMAEMA, also PEI was used to create comb-like polymers, which showed higher transfection efficiencies or at least less cytotoxicity than high molar mass PEI. Higher transfection efficiencies and reduced cytotoxicities compared to PEI or linear PDMAEMA were achieved.…”

Section: Graft Polymersmentioning

confidence: 99%

The influence of polymer architecture on in vitro pDNA transfection

et al. 2015

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In 2012, the first gene therapy agent was approved by the Europe Medicines Agency leading to increased interest in this research field. Beside viruses, non-viral agents based on lipids or polymers represent aspiring alternatives to deliver the genetic material. Different hurdles have to be overcome depending on the kind of nucleic acid used, where plasmid DNA (pDNA) and small interfering RNA represent the common ones. The main challenge for transfection agents, in particular for pDNA delivery, is the transfer to the cell and into the cell nuclei. Within the group of transfection vesicles, cationic polymers show promising features and variability, as they can be synthesized with tailor-made physical and chemical properties (architectures and functionalisation). In the field of polymer-based gene delivery, the tuning potential of polymers by using different architectures like graft and star-shaped polymers as well as self-assembled block copolymers is immense. In particular, in the last few years numerous new polymer designs showed enhanced transfection properties in combination with good biocompatibility. Furthermore, new insights into the transfection mechanism demonstrated the continuous progress in this field. Polymer architecture influences the polyplex characteristics and the latter has an impact on the transfection mechanism, e.g. the interaction with the cellular membrane depends on the polyplex shape. Moreover, polyplex dissociation can be easily influenced by the polymer chemistry, thus biodegradable linkers lead to well suited polymers with reduced toxicity and high delivery potential, and are also promising for in vivo applications. This review focuses on the influence of polymer architectures for pDNA transfection in vitro, showing recent developments and insights. The theoretical background concerning the biological challenges for cationic polymers and the impact of graft-or star-shaped architectures as well as self-assembled structures will be presented in detail.

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Biocleavable comb-shaped gene carriers from dextran backbones with bioreducible ATRP initiation sites

Cited by 78 publications

References 23 publications

Bioreducible POSS-Cored Star-Shaped Polycation for Efficient Gene Delivery

Bioreducible POSS-Cored Star-Shaped Polycation for Efficient Gene Delivery

Recent advances in nanocellulose for biomedical applications

The influence of polymer architecture on in vitro pDNA transfection

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