Biochezmical Interfering Factors and Blood Cells Indices

Hosseini, Hosnie; Dorgalaleh, Akbar; Tabibian, Shadi; Kashiri, Meysam; Moghaddam, Esmaeil Sanei; Alizadeh, Shaban; Bamedi, Taregh; Reykande, Samira Esmaeili; Dorgalaleh, Saeed

doi:10.5812/thrita.15516

Cited by 4 publications

(2 citation statements)

References 14 publications

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“…A key limitation of our study is the small sample size that could have affected our ability to observe statistical significance in the diagnostic performance of other hemogram parameters. Also, the MCV measurement is affected by hyperlipemia, hyperglycaemia, uremia and other biochemical factors which we did not evaluate for [47] .…”

Section: Discussionmentioning

confidence: 99%

Sensitivity and specificity of the mean corpuscular volume and CD4/CD8 ratio in discriminating between rifampicin resistant and rifampicin sensitive tuberculosis

Baluku

Musaazi

Mulwana

et al. 2020

Journal of Clinical Tuberculosis and Other Mycobacterial Diseas

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Background There is need for simple, cost effective and widely available point of care tests for low level health facilities in developing countries to screen for drug resistant tuberculosis (TB) after bacteriological confirmation of TB by smear microscopy. We evaluated the sensitivity and specificity of the mean corpuscular volume (MCV) and CD4/CD8 ratio in discriminating between rifampicin resistant (RR-TB) and rifampicin sensitive (RS-TB) tuberculosis. Methods We performed a secondary analysis of data from a cross sectional study that enrolled adult participants with bacteriologically confirmed pulmonary TB at a national tuberculosis treatment center in Uganda. Blood samples were tested for CD4 and CD8 cell counts, HIV serology and a full hemogram. Rifampicin sensitivity and the bacillary load grade were determined by Xpert MTB/RIF®. Fifty-five participants that had RR-TB (cases) were matched with 110 participants that had RS-TB (controls) for age, sex and HIV status in a ratio of 1:2 respectively. Sensitivity (Se), specificity (Sp), area under curve (AUC) analysis and determination of optimal cut-offs were performed using receiver operating characteristic curves. Results Cases differed from controls with respect to residence (p = 0.031), bacillary load grade (p < 0.010) and MCV (p = 0.021). The Se, Sp and AUC of the MCV (cut-off of > 74.6 femtolitres (fl)) were 88.9%, 34% and 0.607 (p = 0.021) respectively for RR-TB. Among HIV positive participants, the respective Se, Sp and AUC of the MCV for RR-TB (cut-off of > 72.5 fl) were 97.2%, 22.2% and 0.608 (p = 0.061). The respective Se, Sp and AUC of the CD4/CD8 ratio (cut-off of > 0.40) were 67.3%, 50.0% and 0.559 (p = 0.199) on the overall and 54.1%, 71.6% and 0.628 (p = 0.024) among the HIV positive participants for RR-TB. Conclusion The MCV had a high sensitivity but very low specificity for RR-TB. The CD4/CD8 ratio had a low sensitivity and specificity for RR-TB among HIV positive individuals. The utility of either test is low due to low diagnostic accuracy.

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Section: Discussionmentioning

confidence: 99%

Sensitivity and specificity of the mean corpuscular volume and CD4/CD8 ratio in discriminating between rifampicin resistant and rifampicin sensitive tuberculosis

Baluku

Musaazi

Mulwana

et al. 2020

Journal of Clinical Tuberculosis and Other Mycobacterial Diseas

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“…Hyperglycemia damages the structure of erythrocytes (MAZZANTI et al, 1992, BUYS et al, 2013 and increases the level of MCV (HOSSEINI et al, 2014). Tumor cells produce and release many cytokines that increase the accumulation of leukocytes and PLTs (PRANDONI et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Rouleaux formation of white blood cells and platelets in leukemia

Ullah¹,

Naeem²,

Akhtar³

et al. 2018

Biosci. J.

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ABSTRACT:The objective of this study was to measure the effects of glucose and salt level on white blood cells, red blood cells and platelets (PLTs) in the blood of a leukemic patient by using a white light microscope. Different concentrations of glucose and salt in the range of 0 mM to 500 mM were admixed in the blood sample to prepare blood smear. We revealed that shape of erythrocytes, leukocytes and platelets changes and form aggregates. Increasing concentrations of glucose cause to increases aggregation process of white blood cells, red blood cells and platelets. And the increasing concentration of sodium chloride causes to increase rouleaux formation and aggregation of platelets but dehydration due to increased sodium chloride concentration causes to break the aggregation of white blood cells.Comparison of CBC reports of these samples with and without analytes shows that total leukocyte count (TLC) decreases gradually towards normal ranges of leukocytes which is favorable in the treatment of leukemia but at the same time decreasing level of hemoglobin HGB, mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC) and increasing level of red blood cell (RBCs) causes to reduce oxygen supply which is in favor of cancer growth and anemia. This work provides us the base for translation this in vitro study towards the in vivo case of blood microvasculature as a non-invasive methodology.

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Laboratory Assessment of Anemia

Kundrapu

Noguez

2018

Advances in Clinical Chemistry

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Biochezmical Interfering Factors and Blood Cells Indices

Cited by 4 publications

References 14 publications

Sensitivity and specificity of the mean corpuscular volume and CD4/CD8 ratio in discriminating between rifampicin resistant and rifampicin sensitive tuberculosis

Sensitivity and specificity of the mean corpuscular volume and CD4/CD8 ratio in discriminating between rifampicin resistant and rifampicin sensitive tuberculosis

Rouleaux formation of white blood cells and platelets in leukemia

Laboratory Assessment of Anemia

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