Biochemistry of α-D-Galactosidic Linkages in the Plant Kingdom

Dey, Prakash M.

doi:10.1016/s0065-2318(08)60023-2

Cited by 58 publications

(16 citation statements)

References 465 publications

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“…An important group of a-galacto-oligosaccharides known as the raffinose family of oligosaccharides (RFO) are found in appreciable concentrations in pulses and other legumes, most notably soybeans. The biosynthesis of these compounds (also generally referred to as a-galactosides) and their roles as seed storage carbohydrates and in desiccation tolerance were elucidated in detail by Dey (1980) and reviewed by Peterbauer and Richter (2001).…”

Section: A-galacto-oligosaccharidesmentioning

confidence: 99%

Dietary fibres in pulse seeds and fractions: Characterization, functional attributes, and applications

Tosh

Yada

2010

Food Research International

407

246

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Section: A-galacto-oligosaccharidesmentioning

confidence: 99%

Dietary fibres in pulse seeds and fractions: Characterization, functional attributes, and applications

Tosh

Yada

2010

Food Research International

407

246

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“…The primary role of a-galactosidases in the storage organs of plants is to mobilize the reserve a-D-galactosyl-containing oligo-and polysaccharides (5,208,212,290). In fenugreek seeds (228,229,251,302), a low level of the enzyme exists in the early stage of germination.…”

Section: E Rolementioning

confidence: 99%

Biochemistry of the Multiple Forms of Glycosidases in Plants

1984

Advances in Enzymology - And Related Areas of Molecular Biology

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“…Dois tipos de carboidratos são armazenados no endosperma de leguminosas: oligossacarídeos de rafinose (RO) nos vacúolos e galactomananas nas células de parede (DEY, 1980). Os RO são formados de uma molécula de sacarose cujo resíduo glicosil está ligado com um a nove resíduos de galactose por ligação α-(1,6).…”

Section: Introductionunclassified

Purificação e caracterização de alfa-galactosidases de sementes de Platymiscium pubescens Micheli

et al. 2005

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2RESUMO -Este trabalho objetivou foi determinar a composição bioquímica de sementes de espécies florestais e caracterizar a enzima α-galactosidase de sementes germinadas de Platymiscium pubescens. Os maiores teores de lipídios foram determinados em sementes de Chorisia speciosa, Caesalpinia peltophoroides, Tabebuia serratifolia e Tabebuia velanedae, enquanto sementes de Enterolobium contortisiliquum, Schizolobium parahyba e Cassia grandis apresentaram os maiores teores protéicos. A α-galactosidase catalisa a hidrólise dos oligossacarídeos de rafinose, em sementes de leguminosas, durante a germinação. A maior atividade da α-galactosidase foi detectada em sementes de Platymiscium pubescens após 72 h de embebição. Duas formas de α-galactosidases, C1 e C2, foram purificadas de sementes germinadas de P. pubescens, usando-se fracionamento com sulfato de amônio e cromatografias de filtração em gel e de afinidade. Essas enzimas apresentaram atividade máxima em pH 5,5 e a 50-55 °C. Os valores de K m ap das formas C1 e C2, para o substrato ρ-nitrofenil-α-D-galactopiranosídeo, foram de 0,54 mM e 0,78 mM, e para a rafinose, de 4,64 mM e 5,09 mM, respectivamente. Essas enzimas exibiram estabilidade térmica moderada, mantendo 70% da atividade original após 3 h de incubação a 45 °C. A atividade enzimática da C1 e C2 foi totalmente perdida na presença de CuSO 4 e dodecil sulfato de sódio (SDS). Tais enzimas também hidrolisaram melibiose, rafinose e estaquiose, indicando potencial para aplicações biotecnológicas.Palavras-chave: Platymiscium pubescens, α-galactosidase, sementes, germinação e purificação. PURIFICATION AND CHARACTERIZATION OF α α α α α-GALACTOSIDASES FROM Platymiscium pubescens Micheli SEEDSABSTRACT -The objective of this work was to determine seed biochemical composition of forest species and to characterize α-galactosidase enzyme of germinated seeds of Platymiscium pubescens. The highest lipid levels were found in seeds of Chorisia speciosa, Caesalpinia peltophoroides, Tabebuia serratifolia and Tabebuia velanedae, whereas seeds of Enterolobium contortisiliquum, Schizolobium parahyba and Cassia grandis showed the highest protein levels. α-galactosidase catalyzes the hydrolyzis of raffinose oligossacarides in legume seeds during germination. The highest activity of α-galactosidase was found in seeds of Platymiscium pubescens after 72 h of soaking in the water. Two forms of α-galactosidases, C1 and C2, were purified from germinated seeds of P. pubescens, using partition with ammonium sulfate, and gel filtration and affinity chromatographies. These enzymes presented maximum activity at pH 5.5, 50-55°C. K m ap values in the C1 and C2 forms for ρ-nitrophenyl-α-D-galactopyranoside substrate were 0.54 mM and 0.78 mM, and 4.64

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Biochemistry of α-D-Galactosidic Linkages in the Plant Kingdom

Cited by 58 publications

References 465 publications

Dietary fibres in pulse seeds and fractions: Characterization, functional attributes, and applications

Dietary fibres in pulse seeds and fractions: Characterization, functional attributes, and applications

Biochemistry of the Multiple Forms of Glycosidases in Plants

Purificação e caracterização de alfa-galactosidases de sementes de Platymiscium pubescens Micheli

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