Biochemistry of the Golgi apparatus

Keenan, T.W.

doi:10.1007/s004180050251

Cited by 11 publications

(3 citation statements)

References 156 publications

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“…Many proteins are targeted to the plasma membrane, including human GLUT4 and GLUT12, and are transported from the endoplasmic reticulum through the Golgi apparatus to the cell surface [4], [29]. Human GLUT4 and GLUT12 contain several potential targeting motifs that control their localization.…”

Section: Discussionmentioning

confidence: 99%

Functional Analyse of GLUT1 and GLUT12 in Glucose Uptake in Goat Mammary Gland Epithelial Cells

Zhu

Lin

et al. 2013

PLoS ONE

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Glucose transport, mediated by glucose transporters, is necessary for mammary gland development and lactation. GLUT1 and GLUT12 could both be expressed in the pregnant and lactating mammary gland to participate in the glucose uptake process. In this study, the goat GLUT1 and GLUT12 genes were cloned from Saanen dairy goats and transfected into goat mammary gland epithelial cells to assess their biological functions and distributions. The results showed that both goat GLUT1 and GLUT12 had 12 predicted membrane-spanning helices. Goat GLUT1 and GLUT12 each influenced the mRNA expression of the other transporter and increased the glucose consumption and lactose yield in GLUT1- and GLUT12-transfected goat mammary gland epithelial cells, respectively. The overexpression of GLUT1 or GLUT12 also increased the expression of amino acid transporters SLC1A5, SLC3A2 and SLC7A5 and affected genes expressions in GMGE cells. Using immunofluorescence staining, GLUT1 was detected throughout the cytoplasm and localized to the Golgi apparatus around the nuclear membrane, whereas GLUT12 was mainly distributed in the perinuclear region and cytoplasm. This study contributes to the understanding of how GLUT1 and GLUT12 cooperate in the incorporation of nutrient uptake into mammary gland epithelial cells and the promotion of milk synthesis in the goat mammary gland during lactation.

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Section: Discussionmentioning

confidence: 99%

Functional Analyse of GLUT1 and GLUT12 in Glucose Uptake in Goat Mammary Gland Epithelial Cells

Zhu

Lin

et al. 2013

PLoS ONE

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“…Here we apply the MMM-4Pi system to the imaging of two protein distributions of a live Vero cell, that are reportedly constituents of the Golgi apparatus (Keenan, 1998): UDP-galactosyl: glycoprotein,1,4-b-galactosyl transferase (GalT) and 3 0 -phosphoadenylyl-sulfate: uronyl-2-O-sulfotransferase (2-OST). In doing so, we provide initial evidence for the practicality of 4Pi-microscopy in live mammalian cells.…”

Section: Introductionmentioning

confidence: 99%

4Pi-microscopy of the Golgi apparatus in live mammalian cells

Egner

Verrier

Goroshkov

et al. 2004

Journal of Structural Biology

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“…Electron microscopy and cytochemistry together with in vivo and in vitro biochemical analyses have unraveled aspects of structure and biochemistry (Hermo and Smith 1998;Keenan 1998;Mollenhauer and Morre 1998;Roth and Taatjes 1998), of biosynthetic transport into and inside the Golgi apparatus (Bannykh and Balch 1998;Lippincott-Schwartz et al 1998;Morre 1998;Nickel and Wieland 1998), of targeting of Golgi residential proteins (Gleeson 1998), of endocytotic routes to the Golgi apparatus (Gonatas et al 1998;Pavelka et al 1998), of Golgi-disturbing agents (Dinter and Berger 1998), and of the involvement of the Golgi apparatus in neuronal diseases (Gonatas et al 1998).…”

Section: The Manifold Applicationsmentioning

confidence: 99%

Histochemistry and cell biology: what's in a name?

Komminoth

Zuber

1999

Histochemistry and Cell Biology

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Histochemistry represents an integrative discipline aiming at the in situ detection, localization and functional characterization of cellular and extracellular components in single cells and complex organs. Therefore, the development of new methods and improvement of existing ones continues to be important. This, however, is with the declared intent for their application in the various fields of developmental and cell biology as well as pathology in order to contribute to the solution of open problems. This review summarizes recent advancements in these fields.

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Biochemistry of the Golgi apparatus

Cited by 11 publications

References 156 publications

Functional Analyse of GLUT1 and GLUT12 in Glucose Uptake in Goat Mammary Gland Epithelial Cells

Functional Analyse of GLUT1 and GLUT12 in Glucose Uptake in Goat Mammary Gland Epithelial Cells

4Pi-microscopy of the Golgi apparatus in live mammalian cells

Histochemistry and cell biology: what's in a name?

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