Biochemistry of nitrilotriacetate degradation in the facultatively denitrifying bacterium TE11

Iminodiacetate (IDA) is a xenobiotic intenn.ediate common to both aerobic a.nd anaerobic metabolism of nitrilotriacetate (NT A). It is formed by either NTA monooxygenase or NTA dehydrogenase, In this paper the detection and characterization of a membrane-bound iminodiacete dehydrogenase (IDA-DH) from Che/atobacter heintzii ATCC 29600 is reported, which oxidizes IDA to glycine and glyoxylate. Out of 15 compounds tested, IDA was the only substrate for the enzyme. Optimum activity of IDA-DH was found at pH 8.5 and 25°C, respectively, and the Km for IDA was found to be 8mM. Activity of the membrane-bound enzyme was inhibited by KCN, antimycine and dibromomethylisopropyl-benzoquinone. When inhibited by KCN IDA-DH was able to reduce the artificial electron acceptor iodonitrotetrazolium (INT). It was possible to extract IDA-DH from the membranes with 2% cholate, to reconstitute the enzyme into soybean phospholipid vesicles and to obtain IDA-DH activity (more than 50% recovery) using ubiquinone 0 1 as the intermediate electron carrier and INT as the final electron acceptor. Growth experiments with different substrates revealed that in all NTA-degrading strains tested both NTA monooxygenase and IDA-DH were only expressed when the cells were grown on NTA or IDA. Furthermore, in Cb. heintzii ATCC 29600 growing exponentially on succinate and ammonia, addition of0.4g 1~1 NTA led to the induction of the two enzymes within an hour and NTA was utilized simultaneously with succinate. The presence of IDA-DH was confirmed in ten different NTA-degrading strains belonging to three different genera.

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Biochemistry of nitrilotriacetate degradation in the facultatively denitrifying bacterium TE11

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Mathematical description of microbiological reactions involving intermediates

Mathematical description of microbiological reactions involving intermediates

Characterization of an inducible, membrane-bound iminodiacetate dehydrogenase from Chelatobacter heintzii ATCC 29600

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