Biochemistry of dystrophic muscle. Mitochondrial succinate-tetrazolium reductase and adenosine triphosphatase

Pennington, R. J.

doi:10.1042/bj0800649

Cited by 1,030 publications

(251 citation statements)

References 21 publications

(16 reference statements)

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“…Mucosal scrapings were homogenized at 4 ºC in a buffer containing 100 mmol/L mannitol, 2 mmol/L HEPES/Tris, pH 7.1, 0.1 mmol/L benzamidine and 0.2 mmol/L phenylmethylsulfonyl fluoride, using a Waring Blender at maximum speed for 30 s. The activities of the apical membrane enzymes, sucrase, lactase and maltase in the intestinal mucosal homogenates were measured by DAHLQVIST method (5), whereas alkaline phosphatase was estimated by BRE-TAUDIERE method (3). Acid phosphatase, as a marker enzyme of lysosomal membranes, and succinate dehydrogenase, as a marker of mitochondrial membranes, were also determined as previously described (1,21). Protein was measured by Coomassie Brilliant Blue protein assay using bovine gamma-globulin as standard (2).…”

Section: Methodsmentioning

confidence: 99%

Influence of Microcystin-LR on the activity of membrane enzymes in rat intestinal mucosa

Moreno

Mate

Repetto

et al. 2003

J. Physiol. Biochem.

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I. M. MORENO, A. MATE, G. REPETTO, C. M. VÁZQUEZ and A. M. CAMEÁN. Influence of Microcystin-LR on the activity of membrane enzymes in ratintestinal mucosa. J. Physiol. Biochem., 59 (4), [293][294][295][296][297][298][299][300] 2003.The objective of the present study was to evaluate the effects of microcystin-LR (MCLR) on the activity of membrane enzymes from intestinal mucosa. In addition, serum chemistry and peroxidative status of both serum and intestinal homogenate were evaluated after treatment with MCLR. Wistar rats were treated with intraperitoneal injection of either 100 µg pure MCLR/Kg body weight or saline solution. A significant increase in liver weight and altered serum enzyme activities were found in MCLR-treated rats, indicating damage to the liver in these rats, as previously suggested. A higher specific activity of sucrase (1.5-fold) was observed after the administration of MCLR, whereas other intestinal apical membrane enzymes, such as lactase, maltase and alkaline phosphatase were not modified by the treatment. The specific activities of acid phosphatase and succinate dehydrogenase, markers for lysosomal and mitochondrial membranes, respectively, were also increased (32% and 60%, respectively) in treated rats. The analysis of lipid peroxidation showed that the peroxidative status was increased in both serum and intestinal mucosa from MCLRtreated rats, reflecting an excess production of oxygen free radicals induced by this cyanobacterial toxin. In conclusion, this study shows that acute exposure to MCLR affects the intestinal physiology by modifying the intestinal peroxidation status as well as the activity of membrane enzymes.

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Section: Methodsmentioning

confidence: 99%

Influence of Microcystin-LR on the activity of membrane enzymes in rat intestinal mucosa

Moreno

Mate

Repetto

et al. 2003

J. Physiol. Biochem.

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“…Activities of citrate synthase, 34 succinate dehydrogenase, 35 NADH-cytochrome C reductase (complex I-III) and succinate-cytochrome C reductase (complex II-III) were determined as per the earlier methods described. 36,37 Mitochondrial membrane potential.…”

Section: Mitochondrial Dysfunctionsmentioning

confidence: 99%

Evidence of oxidative stress and mitochondrial dysfunctions in the testis of prepubertal diabetic rats

Chandrashekar

Muralidhara

2009

Int J Impot Res

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Earlier, we have shown the occurrence of oxidative impairments and their progression in the testis of diabetic adult rats. This study investigated the vulnerability of immature testis to oxidative stress and mitochondrial dysfunctions in a prepubertal (PP) diabetic rat model. PP male rats (4/6-weekold) rendered diabetic by an acute dose of streptozotocin were monitored for induction of oxidative stress in testis cytosol/mitochondria. Diabetic rats of both age groups showed severe hyperglycemia, testicular atrophy and marked oxidative damage as evidenced by enhanced generation of reactive oxygen species, hydroperoxide and malondialdehyde levels (4 week46 week). Mitochondrial dysfunctions manifested as reduction in the activities of aldehyde dehydrogenase, tricarboxylic acid cycle enzymes, enhanced activities of oxidative phosphorylation enzymes, perturbations in calcium homeostasis and membrane potential. These evidences suggest that an immature testis is vulnerable to oxidative stress under diabetes, which may play a significant role in the development of testicular degeneration, leading to impaired fertility in adulthood.

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“…mined by the fluorimetric ~rocedure or Kint [15]; the plasma membrarte rnarker ~'.nucleotidase (E.C, 3.1 3,5) by the method of lpata [16], as perfected by Melntosh and Plummer [171', the mitochondrial marker succinate dehydrogenase (E.C. 1.1.1.49~ by the method of Oennmgton [18]; the cytosol markers lactate dehydrogenase (E,C. I, 1,1.27) and glucose.…”

Section: 4 Determinatio~ Of Marker Enzymesmentioning

confidence: 99%

Occurrence of sialidase activity in two distinct and highly homogeneous populations of lysosomes prepared from the brain of developing mouse

Fiorilli¹,

Siniscalco²,

Chiarini³

et al. 1991

FEBS Letters

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Lillht and l~eavy lyxo=~ome~ of mou~,= forebrain were s~=parated from ¢a¢11 other hy ¢enlrtfugalion ~n a Pereoll gradient, Light lysosomes were then freed from mitoehondria and membrane~ by stterose density Bl'adieat eentriful~atica and further puritied by floalatton-eentrifuBalion on a sucrose tlradient, "['he final prcparation~ of liiht and heavy ly~osom~, Fairly homogenous, carried sialidase activity, assayed on MU-NeuA¢. The optimal NI wa~ 4,0 and 4.2, the apparent g.~ value 2.8 x 10 =~ M and 4,2 x i0 "~ M and tire apparent 1~ value 0.11 and 0.47 mU, mj =~ protoin, for the liBht and heavy ly~to~ome sialidas¢, respectively, From 4 day~ t0 adulthood the sp,.~ific ac~ivily of the li~,ht and heavy iysosome lialidase increased 3.fold and I

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Biochemistry of dystrophic muscle. Mitochondrial succinate-tetrazolium reductase and adenosine triphosphatase

Cited by 1,030 publications

References 21 publications

Influence of Microcystin-LR on the activity of membrane enzymes in rat intestinal mucosa

Influence of Microcystin-LR on the activity of membrane enzymes in rat intestinal mucosa

Evidence of oxidative stress and mitochondrial dysfunctions in the testis of prepubertal diabetic rats

Occurrence of sialidase activity in two distinct and highly homogeneous populations of lysosomes prepared from the brain of developing mouse

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