Biochemistry and toxicology of proteins and peptides puriﬁed from the venom of Vipera berus berus

Siigur, Jüri; Siigur, Ene

doi:10.1016/j.toxcx.2022.100131

Cited by 6 publications

(15 citation statements)

References 97 publications

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“…During centrifugation, the tubes were open so as not to prevent oxygen access to the platelets. Southeastern European Medical Journal, 2022; 6 (2) Platelet-poor plasma was obtained by centrifugation at 450 g for 20 min at a temperature lower than 20 °С.…”

Section: B) Human Blood Plasmamentioning

confidence: 99%

“…The amount of 50 μL of the suspension was added with the studied sample (total volume -1 mL), mixed, and incubated at 37 °С for 30 min. Southeastern European Medical Journal, 2022; 6 (2) After incubation, the red blood cells were precipitated through centrifugation at 1000 g for 10 min and examined the supernatant's absorbency at 543 nm against the buffer. Hemolysis caused by 50 μL of 1% Triton-X100 was treated as 100%.…”

Section: F) Establishing Hemolytic Activitymentioning

confidence: 99%

“…Evolutionary, venomous reptiles have developed different routes to hit the prey, one or a few of which appeared to be more successful and thus started to prevail. Most snake venoms have neurotoxic action [1]; however, some snakes kill their prey by inducing generalized inflammatory tissue response, or by using blood coagulation activators, which cause massive coagulation or conversely cause hemolysis, inability of the blood to clot, destroy blood vessels and lead to internal hemorrhages [2].…”

Section: Introductionmentioning

confidence: 99%

“…In particular, the proteases destroying fibrinogen, platelet aggregation inhibitors, activators of blood coagulation factors, phospholipases, etc. were found in snake venoms [2]. Obtaining and describing these components led to the discovery of unique enzymes, used nowadays in medicine and laboratory diagnostics.…”

Section: Introductionmentioning

confidence: 99%

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Fractionation of Vipera berus berus Snake Venom and Detection of Bioactive Compounds Targeted to Blood Coagulation System

Iskandarov

Gryshchuk

Platonov

et al. 2022

Southeast. Eur. med. j. (Online)

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Introduction. The performed research focused on a search for new biologically active compounds acting on blood coagulation system proteins and cells. To achieve this goal, we fractionated Vipera berus berus snake venom and studied the action of the separated fractions on human blood plasma, fibrinogen, platelets or red cells. Methods. Crude venom was fractionated using ion-exchange chromatography. Protein composition of fractions was studied using SDS-PAGE. The ability of fractions to prolong or initiate blood plasma clotting was studied using the prothrombin time test with thromboplastin. Fibrinogen-specific proteases were detected using enzyme-electrophoresis. Action on red cells was estimated using the hemolysis test. Aggregometry was used for the detection of action on platelets. All experiments in this study were performed in vitro. Results. We obtained fractions containing phospholipase and a protease that is able to hydrolyze fibrinogen, leading to the loss of its ability to polymerize and to maintain platelet aggregation. Conclusion. Further purification and study of these components can be a promising research direction for biotechnological as well as for biomedical use.

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Section: B) Human Blood Plasmamentioning

confidence: 99%

Section: F) Establishing Hemolytic Activitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Fractionation of Vipera berus berus Snake Venom and Detection of Bioactive Compounds Targeted to Blood Coagulation System

Iskandarov

Gryshchuk

Platonov

et al. 2022

Southeast. Eur. med. j. (Online)

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“…One of the youngest evolutionary forms is the genus Vipera , or True vipers [ 11 ]. Particularly rapid speciation of this genus took place during the Pliocene and Pleistocene [ 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Cytogenetic Analysis of the Bimodal Karyotype of the Common European Adder, Vipera berus (Viperidae)

Spangenberg

Redekop

Simanovsky

et al. 2022

Animals

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Vipera berus is the species with the largest range of snakes on Earth and one of the largest among reptiles in general. It is also the only snake species found in the Arctic Circle. Vipera berus is the most involved species of the genus Vipera in the process of interspecific hybridization in nature. The taxonomy of the genus Vipera is based on molecular markers and morphology and requires clarification using SC-karyotyping. This work is a detailed comparative study of the somatic and meiotic karyotypes of V. berus, with special attention to DNA and protein markers associated with synaptonemal complexes. The karyotype of V. berus is a remarkable example of a bimodal karyotype containing both 16 large macrochromosomes and 20 microchromosomes. We traced the stages of the asynchronous assembly of both types of bivalents. The number of crossing-over sites per pachytene nucleus, the localization of the nucleolar organizer, and the unique heterochromatin block on the autosomal bivalent 6—an important marker—were determined. Our results show that the average number of crossing-over sites per pachytene nucleus is 49.5, and the number of MLH1 sites per bivalent 1 reached 11, which is comparable to several species of agamas.

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Why to Study Peptides from Venomous and Poisonous Animals?

Oliveira

Soares

Silva

2023

Int J Pept Res Ther

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Venom and poison peptides are powerful biological weapons and have proven immense pharmacological potential because of their high binding affinity to a wide range of molecular targets. Nonetheless, many of these peptides cannot directly be used as medicines due to their toxicity but their derivatives are very valuable to explore and can be a great treasure trove for the development of novel drugs. This review presents a detailed overview of venom peptides present in reptiles, amphibians, arachnids, gastropods, clitellatas, fish, insects, and mammals. We address the most recent findings that underline their therapeutic potential against a wide variety of diseases from cancer to vascular, autoimmune, and inflammatory diseases. Graphical Abstract

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Biochemistry and toxicology of proteins and peptides puriﬁed from the venom of Vipera berus berus

Cited by 6 publications

References 97 publications

Fractionation of Vipera berus berus Snake Venom and Detection of Bioactive Compounds Targeted to Blood Coagulation System

Fractionation of Vipera berus berus Snake Venom and Detection of Bioactive Compounds Targeted to Blood Coagulation System

Cytogenetic Analysis of the Bimodal Karyotype of the Common European Adder, Vipera berus (Viperidae)

Why to Study Peptides from Venomous and Poisonous Animals?

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