Biochemistry and Applications of Alcohol Oxidase from Methylotrophic Yeasts

Amperometric Biosensors for Lactate, Alcohols, and Glycerol Assays in Clinical Diagnostics

“…22 is 1.94±0.37 mM for EtOH, a value which is in good agreement with the value of 2.7 mM for AOX in solution (Woodward et al, 1990). The typical signal rise time (95 % of the steady state value at an ethanol concentration of 4 mM) was determined to be 45 s. The optimal working pH value in phosphate buffer was determined to be 7.6.…”

“…Apparently, since AOX is unstable at pH-values below 6.5, the enzyme was denatured due to local proton generation. On the other hand, as suggested by the properties of AOX in solution (Woodward, 1990;Patel et al, 2001), AOX should be resistant to alkaline pH-values. Thus, integration of AOX into a cathodic EDP (CP) was performed using potential pulses to -1200 mV (vs. Ag/AgCl), leading to the generation of OH --ions close to the electrode surface.…”

Formaldehyde Oxidizing Enzymes and Genetically Modified Yeast Hansenula polymorpha Cells in Monitoring and Removal of Formaldehyde

“…Alcohol oxidase (А Х) from the thermotolerant methylotrophic yeast cells H. polymorpha can be an alternative to FdDH, used for analytical purposes. This enzyme is quite stable, contains tightly bound FAD and does not need any exogenous co-enzyme for catalytic activity (Woodward J., 1990). Theoretically, AOX can be used to assay FA because in aqueous solutions FA exists in hydrated form (95-99% of total concentration) which structurally resembles methanol, and can be oxidized by AOХ with the subsequent formation of formic acid and hydrogen peroxide (see reactions 4 and 5).…”

Simple and Rapid Determination of Ethanol Content in Beer Using an Amperometric Biosensor

“…Alcohol oxidase is catalytically active for alcohols containing short alkyl chains, alcohol dehydrogenase is more selective for alcohols other than methanol as the corresponding reaction rate decreases rapidly with the size of the molecule [12]. As discussed in a recent review [13], the use of alcohol oxidase enzyme on the analysis of ethanol in complex samples allows a considerable enhancement in specificity.…”