Biochemische Arbeitsmethoden

Jaenicke, Rainer; Wieme, R.J.; Hecker, E.; Lattrell, R.; Berg, Hermann; Grubhofer, N.; Wacker, A.; Träger, L.; Bayer, Edward A.; Stahl, E.; Determann, Helmut; Gelotte, Bertil; Mahling, A.; Hannig, Kurt; Heide, K.

doi:10.1007/978-3-642-85767-6_12

Cited by 1 publication

(2 citation statements)

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“…7). In view of possible errors involved in the sedimentation analysis and in the various assumptions in ___ calculating the molecular weight (10,18), the BSA result is in good agreement with the structure derived from electron microscopy and optical diffraction data (Fig. 4b).…”

Section: Discussionsupporting

confidence: 74%

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Structure of plain and complex flagellar hooks of Pseudomonas rhodos

Raška¹,

Mayer²,

Edelbluth³

et al. 1976

J Bacteriol

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The proximal hooks of plain and complex flagella produced by a strain of Pseudomonas rhodos have been analyzed by electron microscopy and optical diffraction and filtering. Plain flagellar hooks are cone-shaped, 70 nm long, and 13 to 21.5 nm wide, and consist of helically arranged subunits. Complex flagellar hooks are cylinders, 180 to 190 nm long, and 15 to 16 nm wide, and are composed of globular subunits. The structure comprises four small-scale helical rows of subunits intersecting between 10 and 11 large-scale helices of pitch angle 800. The axial and lateral dimensions of the unit cell, which define the surface lattice, are 4.9 and 4.7 nm, respectively. In addition, a core structure, approximately 5 nm wide, has been demonstrated inside the hook cylinder. Complex flagellar hooks were isolated and purified by gradient centrifugation after acid degradation of the attached filaments. Isolated hook particles have an average sedimentation constant of 130S and consist of a protein of molecular weight 43,000. A model of the complex flagellar hook is presented, and its possible role in flagellar assembly and rotation is discussed.

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Section: Discussionsupporting

confidence: 74%

“…lb) is essentially one-sided. The principal reflections correspond to two sets of intersecting helices of pitch angles 10 and 250, respectively. This subunit arrangement becomes obvious in the one-sided optical reconstruction ( Fig.…”

Section: Gross Morphology Of the Proximal Hooksmentioning

confidence: 99%