Biochemie der Mitose

Selective Inhibition of Cell Cycle Stages in the Allium Root Meristem by Colchicine and Growth Regulators

“…Colchicine can also affect the cell at several levels. Chiefly it destroys the spindle microtubules by binding to a specific protein fraction (Borisy and Taylor, 1967;Duspiva, 1971), but COL also affects DNA synthesis (Fitzgerald and Brehaut, 1970;Vasiliev, Gelfand, and Guelstein, 1971). Moreover, various antagonistic effects have been described between plant growth regulators and colchicine Mac-Leod and Davidson, 1966;Atsmon et aI., 1968;Fragata, 1970).…”

“…346 ONE WAY of studying the possible regulatory mechanisms of the cell cycle is the experimental inhibition and stimulation of specific phases of the cycle. Several investigations indicate that the transition of the nucleus from one stage to another depends on specific RNA synthesis as well as the balanced content of cellular growth hormones (Torrey, 1965;Torrey, Fosket, and Hepler, 1971;Atsmon, Light, and Lang, 1968;MacLeod and Davidson, 1968;Stern, 1969;Duspiva, 1971;Grundmann, 1971). Since the influence of the primary action of phase-specific genes is greatly complicated by the all-or-none effect of drugs which influence RNA or protein synthesis, the analysis of more secondary steps of the gene-action system is preferred.…”