Biochemical tests to determine the biodegradability potential of bacterial strains in PAH polluted sites

Naeim, Amin Hossein; Baharlouei, Jila; Ataabadi, Mitra

doi:10.1007/s11274-020-02950-y

Cited by 7 publications

(2 citation statements)

References 33 publications

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“…Meanwhile, the genus Staphylococcus has been little studied for its potential in PCB biodegradation processes, but there are reports that demonstrate its degradation capability and its application in the bioremediation of hydrocarbons and other contaminants [41][42][43][44]. The genus Bacillus has been one of the most representative, and it has been identified in sites contaminated with PCBs and hydrocarbons [45,46]. Bacillus has been reported by several authors to be a genus with significant capacities for the tolerance and assimilation of these compounds [47,48], while Bacillus subtilis has been extensively studied based on the biological mechanisms involved in the metabolism of PCBs and the genes involved [49].…”

Section: Discussionmentioning

confidence: 99%

Biostimulation and Bioaugmentation of Soils Contaminated with Decachlorobiphenyl (PCB-209) Using Native Bacterial Strains Individually and in Consortia

et al. 2022

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Historically, microorganisms have proven to be efficient alternatives for the removal of PCBs, since these contaminants continue to be a major problem for human health and the environment. In this work, the removal of decachlorobiphenyl (PCB-209) was evaluated using native bacterial strains individually and in consortia through biostimulation and bioaugmentation processes. Bacillus sp. DCB13, Staphylococcus sp. DCB28, and Acinetobacter sp. DCB104 were biostimulated in a minimal medium that initially contained biphenyl and later PCB-209 for adaptation as a carbon source. The removal potential of PCB-209 by bacterial strains was evaluated in a bioaugmentation process under aerobic conditions. Using a completely randomized design, ten different treatments were evaluated. Finally, the bacterial growth (CFU/g of soil) and the chemical characteristics of the bioaugmented soil were determined, as was the content of PCB-209 removed by gas chromatography–mass spectrometry. Strains DCB13, DCB28, and DCB104 showed cell growth (>3.4 × 105 CFU/mL) during 120 h of biostimulation, with a marked difference between treatments with biphenyl compared with those where PCB-209 was added. Strains DCB13 and DCB104 (3.4 × 105 CFU/mL and 2.0 × 106 CFU/mL, respectively) grew better with PCB-209, while DCB28 grew better with biphenyl (4.5 × 106 CFU/mL). In bioaugmented soils contaminated with PCB-209, the strains showed maximum growth when inoculated in a consortium (>2.0 × 104 CFU/g). The results showe that the range of the bacterial elimination of PCB-209 in the treatments was from 9.58 to 17.33 mg/kg. The highest elimination potential of PCB-209 was obtained when the bacterial strains were inoculated in a consortium. These findings open a wide perspective for the use of native bacteria for the cleaning and restoration of soils contaminated by toxic chemicals.

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Section: Discussionmentioning

confidence: 99%

Biostimulation and Bioaugmentation of Soils Contaminated with Decachlorobiphenyl (PCB-209) Using Native Bacterial Strains Individually and in Consortia

et al. 2022

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“…The results indicated the high ability of M. hydrocarbonoclasticus for the biodegradation of soil PAH. Naeim et al (2020) found that among different bacterial strains, M. hydrocarbonoclasticus had the highest rate of PAH degradation, which was due to the activity of laccase enzyme, and the production of biosurfactant, biofilm and exopolysaccharide (Isfahani et al 2018). The bacteria use pollutants as a source of carbon to produce biosurfactants, which entraps and solubilises the pollutant.…”

Section: Discussionmentioning

confidence: 99%

Different biological strategies for the bioremediation of naturally polluted soils

Naeim¹,

Baharlouei²,

Ataabadi³

2021

PLANT SOIL ENVIRON

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Finding an appropriate method with the highest rate of polycyclic aromatic hydrocarbon (PAH) removal from naturally polluted soils is an important research issue. A pot factorial experiment (using contaminated soil samples from the Isfahan Refinery, Iran) was conducted in a 90-day period to compare the following bioremediation strategies: (1) natural attenuation (NA): the inherent ability of soil for bioremediation; (2) bioaugmentation (BA): inoculating soil with PAH degrading microbes Marinobacter hydrocarbonoclasticus; (3) biostimulation (BS): using N, P and K nutrients for the stimulation of bioremediating soil bacteria to achieve the C : N : P ratio of 100 : 10 : 1, and(4) bioaugmentation + biostimulation (BS + BA). Treatments NA (22.8%) and BA + BS (63.9%) resulted in the least and the highest rate of PAH removal from the soil. The 2–4 ring compounds had a significantly (P ≤ 0.05) higher rate of degradation than the 5–6 ring compounds. The highest rates were resulted by fluorene (76.41%) and acenaphthylene (72.28%) using the BA + BS treatment. However, the lowest degradation rates were resulted by indeno (1,2,3-cd) pyrene (10.05%), benzo [b] fluoranthene (10.17%), benzo (g, h, i) perylene (12.53%), and benzo [k] fluoranthene (13.67%), using NA treatment. The BA + BS treatments are the most effective method for the bioremediation of PAH polluted soils.

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Chromium (VI) bioremoval from contaminated wastewater using Pseudomonas aeruginosa ATHA23 producing biofilm supported on clinoptilolite

Ataabadi

Hoodaji

Tahmourespour

2022

Environ Geochem Health

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Biochemical tests to determine the biodegradability potential of bacterial strains in PAH polluted sites

Cited by 7 publications

References 33 publications

Biostimulation and Bioaugmentation of Soils Contaminated with Decachlorobiphenyl (PCB-209) Using Native Bacterial Strains Individually and in Consortia

Biostimulation and Bioaugmentation of Soils Contaminated with Decachlorobiphenyl (PCB-209) Using Native Bacterial Strains Individually and in Consortia

Different biological strategies for the bioremediation of naturally polluted soils

Chromium (VI) bioremoval from contaminated wastewater using Pseudomonas aeruginosa ATHA23 producing biofilm supported on clinoptilolite

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