Biochemical Tests for Identification of Medical Bacteria

Legolvan, Paul C.

doi:10.1093/milmed/146.1.60

Cited by 27 publications

(35 citation statements)

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“…The result showed that sample A1 had the highest total aerobic plate count with 2.95x10 5 , while the least total aerobic plate counts were recorded in sample E2 with 1.4x10 4 . The result also indicated that sample A2 had the highest total fungal counts of 8x103 while all samples in C recorded the least total fungal counts of 1x10 3 . Additionally, for coliforms count, sample A1 was the highest with 240 coliform counts and sample E2 recorded the least coliforms counts of 3.…”

Section: Resultsmentioning

confidence: 85%

“…Meat processing includes all the processes that change fresh meat with the exception of simple mechanical processes such as cutting, grinding or mixing [2]. According to the guidelines of good manufacturing practices, the level of total microbiological contamination of raw meat and raw meat preparations, it is commonly suggested that microorganisms can enter meat preparation like sausages from meat, spices, and other ingredients, as well as from processing environment, equipment, and handlers that can have a significant impact on the microbiological status of the end-products [3].…”

Section: Introductionmentioning

confidence: 99%

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Microbial Quality Assessment of Processed Meat Product (Tsire) Sold Within Wudil Town, Wudil Local Government Area, Kano State, Nigeria

Yahaya

et al. 2018

MAPP

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Microbial quality assessment of Tsire meat products sold within Wudil town was conducted between February-April 2018. A total of fifteen (15) samples were purchased from five different spots and analyzed microbiologically using the pour plate method. The total aerobic plate count on nutrient agar ranged from 1.4x10 4 cfu/ml to 2.95x10 5 cfu/ml. The coliform count using most probable number technique (MPN) ranged from 3cfu/g-240cfu/g. Total fungal count (yeast and mold) on Sabaroud dextrose agar with Chloramphenicol (as control) ranged from 1x10 3 cfu/ml-8x10 3 cfu/ml. The bacteria isolated include Staphylococcus aureus, Escherichia coli, Shigella spp, and Salmonella spp. The fungal isolates obtained from this study are Penicillium spp, and Aspergillus niger.The percentage of occurrence of bacterial isolates was highest in Staphylococcus aureus (43.5%), Shigella spp (21.7%), Salmonella spp (21.7%) and Escherichia coli (13.0%) recorded the lowest. The percentage of occurrence of fungi isolated from the Tsire samples was highest in Aspergillus niger (66.7%), and Penicillium spp (33.3%). It is concluded that the occurrence of such organisms indicates contamination of the Tsire meat samples. Hence, proper care in the course of preparation and handling of Tsire meat needs to be established. Educating the meat handlers on the issue of food safety and public health will reduce the rate of contamination of the Tsire meat.

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Section: Resultsmentioning

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Microbial Quality Assessment of Processed Meat Product (Tsire) Sold Within Wudil Town, Wudil Local Government Area, Kano State, Nigeria

Yahaya

et al. 2018

MAPP

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“…Inoculated plates were incubated at 37ºC for 24-48 hours and then examined for bacterial growth. Identification of bacteria was performed by standard biochemical tests (catalase, coagulase, mannitol fermentation and D-Nase tests) [23,24].…”

Section: Bacteriological Examinationmentioning

confidence: 99%

Prevalence and Antibiogram of Staphylococcus aureus in Clinical and Subclinical Mastitis in Holstein Dairy Cows in Egypt

Talaat

Beskawy²,

Atwa³

et al. 2023

Zagazig Veterinary Journal

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Mastitis is a multifactorial and ubiquitous disease that results from interactions between the host, environment, and infectious agents leading to extensive economic losses. The objectives of this study were to estimate the prevalence of S. aureus in clinical and subclinical mastitis in Holstein dairy cows and determine the susceptibility of S. aureus isolatesagainst different antibiotics for screening of antibiotic resistance genes. A total of 415 Holstein dairy cows were randomly selected from three Egyptian governorates. Selected cows were examined for clinical and subclinical mastitis during the period from October 2014 to June 2018. Milk samples were examined for the presence of S. aureus. The S. aureus isolates were subjected to antibiotic sensitivity, molecular identification, and detection of the antibiotic resistance gene.The prevalence of S. aureus isolated from milk samples was 15.4% of which, 14.3% in clinical cases, and 15.7% in subclinical cases.The antibiogram of S. aureus isolates against 13 antibiotics using the disc-diffusion method revealed the highest rate of resistance to Oxacillin (OX) (96.7%), followed by Ampicillin (AM),Cefoxitin (FOX) (93.3%, each), Tetracycline (TE) (73.3%), Cefotaxime (CTX) (70%), Ampicillin/Sulbactam (SAM) (66.7%), Erythromycin (E) and Sulphamethoxazole/Trimethoprim (SXT) (56.7%, each), Gentamicin (GM) (53.3%), Ofloxacin (OFX) (40%), Chloramphenicol (C) (36.7%), Ciprofloxacin (CIP) (30%) and finally Vancomycin (VA) (0%). Molecular PCR assay revealed that all the 16 S. aureus isolates (100%) carried mecA gene, while 15 out of 16 isolates (93.7%) carried blaZ genebut, 8 out of 16 (50%) carried tetK gene, and only one isolate (0.06%) carried fexA gene. Uncontrolled uses of antibiotics in the treatment of mastitis should be restricted and increase awareness about the risk of antimicrobial-resistant bacteria in milk.

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“…For the purpose of antimicrobial activity seven microorganisms were used, Psendomonas aerogenosa, Escherichia coli, Proteus sp., Staphylococcus aureus, Klehsieltla sp., Salmonella sp., Streptococcus sp., Microorganisms species were employed in this study obtained from stock cultures of Microbiology laboratory, Department of Microbiology , college of Medicine, university of Kufa. The bacterial species were identified according to (13 ), and maintain on nutrient agar slants and recovered for testing by sub-culturing in nutrient broth for 24hrs. The antimicrobial activity tests were then carried out by agar diffusion assay (14).…”

Section: Antibacterial Activity Assaymentioning

confidence: 99%

Synthesis and study biological activity of some 1,8- Naphthyriane Derivatives

Alkabee

Joad²

2020

Kufa Jour. Bio.

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The compound 4-carboxy -2-phenyl-1,8- naphthyridine was synthesized from pyruvic acid, 2-Aminopyridine and Benzaldchyde (I).4-Hydrocynoamine-2-phenyl-1,8- Naphthyridine synthhesized from 4-Carboxy-2-phenyl-1.8-naphthyridine and phosphor penta chloride anhydrous in carbon tetra chloride, the resultant derivative was then separated by crushed ice and filtrated, the solid acid chloride thus obtained was used for further reaction. to the solid which was form was added hydrazine hydrate (II).4(p-phenyl dimethyl aminc) -hydrazine)-1,8-Naphthyridine (III) was synthesized from refluxed equimolar quantities of (II) with p.Dimethylamino- Benzaldephyde in ethanol. The purity of the synthesized compounds were established through R spectroscopy. and the purity were confirmed by TLC. the physical properties such as melting point and percentage were determined. The antibacterial activity of synthesized compounds (I, II, III) were tested by using agar diffusion method against some bacterial species, the compounds exhibit highest antibacterial activity specially compound III, also the Minimal inhibitory concentration (MIC) of tested compounds (I, II, III) were determined, the results showed that MIC values ranged (5-20) μg/ml for all bacterial species in this study, Pseudomsonas aerogenosa, Escherichia coli,Proteus sp, Staphylococcus aureus, Klebsiella sp., Sallmonella sp. ,Streptococcus sp.

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Biochemical Tests for Identification of Medical Bacteria

Cited by 27 publications

References 0 publications

Microbial Quality Assessment of Processed Meat Product (Tsire) Sold Within Wudil Town, Wudil Local Government Area, Kano State, Nigeria

Microbial Quality Assessment of Processed Meat Product (Tsire) Sold Within Wudil Town, Wudil Local Government Area, Kano State, Nigeria

Prevalence and Antibiogram of Staphylococcus aureus in Clinical and Subclinical Mastitis in Holstein Dairy Cows in Egypt

Synthesis and study biological activity of some 1,8- Naphthyriane Derivatives

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