Biochemical Study of the Protective Effect of Vitamin C and Radish on Gentamicin-Induced Nephrotoxicity in Rats

Altimimi, Murooj L.; Hassan, Ekhlas Sabah; Al-Nafakh, Rana Talib; Alfadhul, Shaymaa Abdul Lteef; Mohammad, Ammar Rasoul

doi:10.5958/0973-9130.2019.00327.x

Cited by 3 publications

(3 citation statements)

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“…A. lanata is commonly employed in Siddha system of medicine and is stated to exhibit a marked protective effect against Geninduced nephrotoxicity in the rat (32,33). Furthermore, the nephroprotective potential of A. paniculata and R. sativus has been confirmed by several studies (26,27,34,35).…”

Section: Discussionmentioning

confidence: 97%

Date palm seed extract and herbal mixture mitigate gentamicin-induced renal injury in mice: Role of Protease-activated receptors (PARs) and Retinoid X receptor alpha (RXR-α)

Sohaim

Mohammed

Amin

et al. 2022

J Herbmed Pharmacol

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Introduction: Gentamicin (Gen) causes renal toxicity by inhibiting protein synthesis in kidney cells, causing proximal tubule cell necrosis and renal failure. Herein, we examined the nephroprotective effect of date palm seed extract (DPSE) and one herbal mixture (HM; composed of Tribulus terrestris, Aerva lanata, Andrographis paniculata, and Raphanus sativus) against Gen-induced renal toxicity in mice with special reference to the possible role of retinoid X receptor alpha (RXR-α) and protease-activated receptor 2 (PAR-2) in this effect. Methods: Thirty-two male Balb/c mice divided randomly into four groups were either treated with saline, Gen (225 mg/kg/i.p., daily from day 3 to day 10), Gen (225 mg/kg i.p.) daily from day 3 to day 10 and DPSE (100 mg/kg/p.o.) daily for 10 days, or Gen (225 mg/kg i.p.) daily from day 3 to day 10 and HM (100 mg/kg/p.o., daily for 10 days). Mice were sacrificed 24 hours after the last dose administration, and kidney tissues were dissected out, weighed, and subjected to histological, immunofluorescence, and biochemical assays. Results: The Gen-induced renal toxicity group demonstrated a significant decrease in RXR-α and a significant increase in PAR-2 protein expression. Treatment with DPSE or HM significantly improved Gen-induced effects on serum creatinine, blood urea nitrogen (BUN), white blood cells (WBCs), platelets, RXR-α extracellular matrix deposition, and PAR-2. Conclusion: The present study stated the nephroprotective effects of DPSE and HM and revealed, for the first time, the involvement of retinoid receptors and PAR-2 in Gen-induced renal toxicity as well as in the protective effects of the two tested natural products.

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Section: Discussionmentioning

confidence: 97%

Date palm seed extract and herbal mixture mitigate gentamicin-induced renal injury in mice: Role of Protease-activated receptors (PARs) and Retinoid X receptor alpha (RXR-α)

Sohaim

Mohammed

Amin

et al. 2022

J Herbmed Pharmacol

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“…On the contrary, VC co-administration concurrently with BLD significantly reduced the renal function impairment. Similarly, Al-Timimi et al (2019) signified the protective effect of VC against gentamicin-induced nephrotoxicity. This protection could be ascribed to a compensatory mechanism involving induction of antioxidant enzyme activities as a defense system by reducing ROS and increasing the nitric oxide to prevent free radical-induced cellular transformation ( Moreira et al, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

Boldenone Undecylenate-Mediated Hepatorenal Impairment by Oxidative Damage and Dysregulation of Heat Shock Protein 90 and Androgen Receptors Expressions: Vitamin C Preventive Role

Behairy¹,

Mohamed²,

Ebraheim³

et al. 2021

Front. Pharmacol.

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Boldenone Undecylenate (BLD) is a synthetic derivative of testosterone and a widely used anabolic androgenic steroid. The health risk of BLD use as a pharmaceutical or dietary supplement is still underestimated and under-reported. Vitamin C (VC) has been recognized as an antioxidant with prominent hepatorenal protective effects. This study investigated the possible preventive activity of VC against BLD-induced hepatorenal damage. Forty adult male Wistar rats were classified into five groups: control, vehicle control, VC (orally given 120 mg/kg b. wt./day), BLD (intramuscularly injected 5 mg/kg b. wt./week), and BLD + VC-treated groups. The experiment continued for eight weeks. Serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured. Serum contents of total protein (TP), albumin (ALB), globulin, total cholesterol (TC), triglycerides (TG), high-density lipoprotein-cholesterol (HDL-C), low-density lipoprotein-cholesterol (LDL-C), and very-low-density lipoprotein–cholesterol (VLDL-C) were also assayed. Urea, creatinine, and uric acid levels were determined together with sodium and potassium electrolytes measuring. Moreover, oxidative stress indicators including reduced glutathione (GSH), glutathione peroxidase (GPx), glutathione-S-transferase (GST), and glutathione reductase (GSR) as well as malondialdehyde (MDA) levels were measured in both hepatic and renal tissues. Corresponding histological examination of renal and hepatic tissues was conducted. Besides, immunohistochemical evaluations for androgen receptors protein (AR) and heat shock protein 90 (Hsp 90) expressions were performed. BLD caused significant rises in serum ALT, AST, TP, ALB, TC, TG, LDL-C, VLDL-C, urea, creatinine, uric acid, potassium, and MDA levels. Further, BLD-injected rats showed significant declines in the serum levels of HDL-C, sodium, GSH, GPx, GST, and GSR. Besides, distinct histopathological perturbations were detected in renal and hepatic tissues of BLD-injected rats. AR and Hsp 90 immunoexpression were increased in hepatic and renal tissues. In contrast, VC significantly reversed the BLD-induced hepatorenal damage in co-treated rats but not ameliorated AR protein overexpression. VC could be an efficient preventive supplement for mitigating BLD-induced hepatorenal damage, possibly via controlling oxidative stress events.

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“…Vitamin C shows protection against nephrotoxicity by a compensatory mechanism involving the induction of antioxidant enzyme activities as a defence against reducing reactive oxygen species and by increasing the levels nitric oxide that prevents free radical-induced cellular transformation. Vitamin C has been proposed as a potent antioxidant agent that is capable of uptake of reactive oxygen species in plasma, thereby, playing a crucial role in the preventing their entry into the cells and subsequent damage of cellular components [21].…”

Section: Discussionmentioning

confidence: 99%

Protective Effects of Alcoholic and Aqueous Extracts of Lyophilized Kiwifruit on Acute Kidney Injury (AKI) Induced by Glycerol in Male Albino Rat

Hakami

Khalifa

Alharbi

2021

JPRI

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Objectives: The study aimed to evaluate and compare the protective effects of alcoholic and aqueous extracts of lyophilized kiwifruit on acute kidney injury (AKI) induced by glycerol in male albino rats. Methods: Rats were divided into four groups: negative control healthy group, acute kidney injury group (administered glycerol), AKI rats treated with ethanolic kiwifruit extract group, and AKI rats treated with aqueous kiwifruit extract group. Kidney biomarkers, oxidative stress, and inflammatory markers were measured and histological examination was performed. Results: We observed that glycerol induced acute renal injury and as a result there was an increase in the levels of blood urea nitrogen, creatinine, Na+, K+, Cl-, uric acid, cystatin-C, neutrophil gelatinase-associated lipocalin, C-reactive protein, tumour necrosis factor-α, interleukin-6, and malondialdehyde as well as a decrease in the levels of reduced glutathione and superoxide dismutase compared to that in the negative control group. Ethanolic and aqueous kiwifruit extracts improved all the kidney biomarkers in AKI rats. Histological examination showed acute tubular necrosis in the AKI group. However, there was an improvement in the renal tissue, represented by epithelial cell regeneration, in rats that were administered both the extracts. Conclusion: Kiwifruit extracts have a positive effect on serum biochemical parameters and renal tissue, which can be beneficial in the treatment of AKI. The treatment with the ethanolic extract is more potent than with the aqueous extract.

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Biochemical Study of the Protective Effect of Vitamin C and Radish on Gentamicin-Induced Nephrotoxicity in Rats

Cited by 3 publications

References 0 publications

Date palm seed extract and herbal mixture mitigate gentamicin-induced renal injury in mice: Role of Protease-activated receptors (PARs) and Retinoid X receptor alpha (RXR-α)

Date palm seed extract and herbal mixture mitigate gentamicin-induced renal injury in mice: Role of Protease-activated receptors (PARs) and Retinoid X receptor alpha (RXR-α)

Boldenone Undecylenate-Mediated Hepatorenal Impairment by Oxidative Damage and Dysregulation of Heat Shock Protein 90 and Androgen Receptors Expressions: Vitamin C Preventive Role

Protective Effects of Alcoholic and Aqueous Extracts of Lyophilized Kiwifruit on Acute Kidney Injury (AKI) Induced by Glycerol in Male Albino Rat

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