Biochemical study of the effects of cilostazol in rats subjected to acute ischemia and reperfusion of hind limbs

Abstract

PURPOSE:To investigate whether cilostazol has a protective effect on acute ischemia and reperfusion of hind limbs of rats through study of biochemical variables in blood and urine. METHODS:Forty six animals were randomized and divided into two groups. Group I received a solution of cilostazol (10 mg/Kg) and group II received saline solution 0.9% (SS) by orogastric tube after ligature of the abdominal aorta. After four hours of ischemia the animals were divided into four subgroups: group IA (Cilostazol): two hours of reperfusion. Group IIA (SS): two hours of reperfusion.Group IB (Cilostazol): six hours of reperfusion. Group IIB (SS) six hours of reperfusion. After the reperfusion period, was held to collect urine and blood for biochemical measurements. The biochemical parameters studied were: urea, creatinine, sodium, potassium and myoglobin in blood and urea, creatinine, myoglobin in urine. RESULTS:There was no statistically significant difference between groups. CONCLUSION:Cilostazol had no protective effect on ischemic acute reperfusion of hind limbs of rats in this model.

Effects of dietary creatine supplementation on kidney and striated skeletal muscles of rats submitted to ischemia and reperfusion of hind limbs

“…The analysis of renal function through the measurement of plasma urea and creatinine is frequently used in experimental models of ischemia and reperfusion 15 , 19 . The significant increase in plasma urea concentration ( Table 1 ) in the group, which did not receive creatine supplementation (G2), may be related to a protective effect against renal reperfusion injuries.…”

“…After this period of reperfusion, the left nephrectomy was performed, the left posterior paw muscle was removed and cardiac puncture was performed to collect 5 mL of blood, a determining factor of euthanasia 15 . The blood was transferred to a dry test tube, which was numbered and sent to the laboratory for processing the serum dosages of urea, creatinine and alanine aminotransferase (ALT).…”