Biochemical studies on the immobilized lactase in the combined alginate–carboxymethyl cellulose gel

Anh, Thi Hai; Tran, Van Nguyen; Le, Van Hieu

doi:10.1016/j.bej.2013.03.003

Cited by 32 publications

(10 citation statements)

References 25 publications

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“…The apparent K m of lactase immobilized in carboxymethyl cellulose-alginate beads was 107.24 mM as compared to 95.57 mM for entrapped lactase in alginate. It might be due to increased surface area of beads and least diffusion resistance faced by substrate to accumulate inside the beads [ 27 ]. The apparent K m (2.08 mM) and apparent V max (0.95 µ mol min −1 ) of immobilized lipase were lower as compared to free lipase ( K m 8.0 mM; V max 2.85 µ mol min −1 ) [ 19 ].…”

Section: Resultsmentioning

confidence: 99%

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Improved Enzyme Catalytic Characteristics upon Glutaraldehyde Cross-Linking of Alginate Entrapped Xylanase Isolated fromAspergillus flavusMTCC 9390

2015

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Purified fungal xylanase was entrapped in alginate beads. Its further cross-linking using glutaraldehyde resulted in large enzyme aggregates which may function as both a catalyst and a support material for numerous substrate molecules. Enzyme cross-linking presented a negative impact on enzyme leaching during repeated washings and recovery of enzyme activity was substantial after twelve cycles of usage. The entrapment followed by cross-linking doubled the total bound activity and also greatly improved the enzyme stability at extreme chemical environment. The wide pH stability, better thermo- and storage stability, lowered K m value, and protection from some metal ions are salient achievements of present immobilization. The study shows the efficacy, durability, and sustainability of immobilized catalytic system which could be efficiently used for various juice processing operations.

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Section: Resultsmentioning

confidence: 99%

“…Our results are in agreement with earlier reports which also provided similar suggestions [ 25 , 36 ]. Researchers have always paid attention to strongly cross-link the enzyme molecules in the polymer network with the help of linkers, aggregators, carriers, and composite materials [ 13 , 27 , 29 , 37 ].…”

Section: Resultsmentioning

confidence: 99%

Improved Enzyme Catalytic Characteristics upon Glutaraldehyde Cross-Linking of Alginate Entrapped Xylanase Isolated fromAspergillus flavusMTCC 9390

2015

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“…Its firmness level was found relatively Beside the wide utilization of oleogels as fat substitutes, they can also be used as carrier materials of bioactive compounds. Several preliminary studies have reported the utilization of gels as carrier materials of carotenoids, polyphenols, flavonoids, omega-3 fatty acids, probiotics, and enzymes [6,20,[26][27][28][29], targeting improved properties and enhanced functionality of the respective bioactive compounds. Thus, further studies should be focused on both physical and functional properties of the produced oleogels, which will lead to successful market applications.…”

Section: Discussionmentioning

confidence: 99%

Olive Oil Oleogel Formulation Using Wax Esters Derived from Soybean Fatty Acid Distillate

et al. 2020

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Oleogelation is an emerging technology to structure oils, which can be widely used to substitute saturated and trans fats. Extra virgin olive oil is widely recognized for its high nutritional value, but its utilization in oleogel production is currently limited. In this study, extra virgin olive oil was utilized for the production of a novel oleogel using wax esters derived from soybean fatty acid distillate (SFAD), a byproduct of industrial soybean oil refining. Different concentrations (7%, 10%, 20%, w/w) of SFAD-wax esters were used to evaluate the minimum concentration requirement to achieve oleogelation. Analyses of the mechanical properties of oleogel showed a firmness of 3.8 N, which was then reduced to around 2.1–2.5 N during a storage period of 30 days at 4 °C. Rheological analysis demonstrated that G′ is higher than G″ at 20–27 °C, which confirms the solid properties of the oleogel at this temperature range. Results showed that SFAD was successfully utilized for the oleogelation of olive oil, resulting in a novel oleogel with desirable properties for food applications. This study showed that industrial fatty side streams could be reused for the production of value-added oleogels with novel food applications.

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“…37,38 In addition, the higher X-intercept of the regression curve in the absence of a magnetic field indicates a lower K m and implies that MIC has higher affinity for the substrate in a nonmagnetic system than in the MTFBR, consistent with previous reports. 39 As shown in Table 1, increasing the magnetic field intensity of the SMF significantly increased the V max of the MICcatalyzed reaction from 2.7601 to 9.4607 mg•min −1 . Similar results for the trends of the K m and V max values of cellulase in a static magnetic field were reported previously.…”

Section: Industrial and Engineering Chemistry Researchmentioning

confidence: 85%

Comparing Immobilized Cellulase Activity in a Magnetic Three-Phase Fluidized Bed Reactor under Three Types of Magnetic Field

Cui

Kou

et al. 2018

Ind. Eng. Chem. Res.

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A novel, magnetic three-phase fluidized bed reactor (MTFBR) was designed with magnetic immobilized cellulase (MIC) as the biocatalyst for preparing chitooligosaccharides from chitosan. The MIC showed higher enzyme activity in a pulse magnetic field (PMF) than that in a steady magnetic field (SMF) or alternating magnetic field (AMF) under certain operating parameters. The application of a magnetic field increased the maximum reaction rate (V max ) and Michaelis constant (K m ), and the reaction rates of the MIC-catalyzed reaction in PMF and SMF at high concentration of chitosan solution (C s , 5 < C s < 20 mg/mL) increased as the intensity of the magnetic field increased. The concentrations of chitosan pentamer and hexamer produced were higher in the PMF than in the SMF and AMF, and the application of a magnetic field greatly reduced (by as much as 37.5%) the biocatalytic reaction time required to reach the maximum concentration of the desired chitooligosaccharides. These results suggest that the customized MTFBR supplemented with MIC is a reusable and effective piece of biocatalytic equipment, indicating that it has potential applications for large industrial-scale chitooligosaccharide syntheses.

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Biochemical studies on the immobilized lactase in the combined alginate–carboxymethyl cellulose gel

Cited by 32 publications

References 25 publications

Improved Enzyme Catalytic Characteristics upon Glutaraldehyde Cross-Linking of Alginate Entrapped Xylanase Isolated fromAspergillus flavusMTCC 9390

Improved Enzyme Catalytic Characteristics upon Glutaraldehyde Cross-Linking of Alginate Entrapped Xylanase Isolated fromAspergillus flavusMTCC 9390

Olive Oil Oleogel Formulation Using Wax Esters Derived from Soybean Fatty Acid Distillate

Comparing Immobilized Cellulase Activity in a Magnetic Three-Phase Fluidized Bed Reactor under Three Types of Magnetic Field

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