Biochemical studies on the entry of sindbis virus into BHK-21 cells and the effect of NH4Cl

Talbot, Pierre J.; Vance, Dennis E.

doi:10.1016/0042-6822(82)90365-8

Cited by 24 publications

(18 citation statements)

References 20 publications

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“…The fact that neutralizing the acidic endosome-lysosome system enhances PCV2 infection of epithelial cells is in contrast with numerous studies that have shown that neutralizing the acidic endosome-lysosome system inhibits the infection of pH-dependent nonenveloped viruses (7,8,18,35,36,63) and enveloped viruses (21,38,64,65,70). Inhibiting endosome-lysosome system acidification also inhibits PCV2 infection of 3D4/31 monocytic cells (51), further contrasting the results presented in this study for epithelial cells.…”

Section: Discussioncontrasting

confidence: 55%

Inhibition of Endosome-Lysosome System Acidification Enhances Porcine Circovirus 2 Infection of Porcine Epithelial Cells

Misinzo

Delputte

Nauwynck

2008

J Virol

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Section: Discussioncontrasting

confidence: 55%

Inhibition of Endosome-Lysosome System Acidification Enhances Porcine Circovirus 2 Infection of Porcine Epithelial Cells

Misinzo

Delputte

Nauwynck

2008

J Virol

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“…This has been confirmed by study of the multiplication of both populations in the presence of chloroquine, an efficient lysosomotropic agent (De Duve et al, 1974). Inhibition of viral multiplication by lysosomotropic agents may act by inhibiting either virus delivery to lysosomes (Talbot & Vance, 1982), or the processing of viral constituents (if they reach the organelle) due to the increase of pH which inhibits the action of the hydrolases and stabilizes the lysosomal membranes (De Duve et al, 1974). In any case, lysosomotropic agents inhibit the multiplication of viruses taken up by adsorptive endocytosis, and not those internalized by fusion.…”

Section: Discussionmentioning

confidence: 53%

Ultrastructural and Biochemical Study of Frog Virus 3 Uptake by BHK-21 Cells

Braunwald

Nonnenmacher

Tripier-Darcy

1985

Journal of General Virology

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SUMMARYUltrastructural studies of the uptake of enveloped and naked frog virus 3 (FV 3) particles by BHK-21 cells have shown that enveloped viruses are internalized by adsorptive endocytosis via coated pits. The enveloped particles then appear to move through endosomes and finally lysosomes. Naked viruses may also follow the same pathway but only rarely. Their more frequent mode of entry is by fusion between the virus shell and the cellular membranes, thus allowing the virus to shed its nucleoprotein content directly into the cytoplasm. This difference in the mechanism of penetration has been confirmed by the use of lysosomotropic agents: the inhibition of viral growth being far more drastic for enveloped FV 3 than for naked virus implies that a lysosomal step is required for the multiplication of enveloped viral particles.

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“…Alternatively, NH4CI may have prevented the delivery of viruses contained in small endocytic vesicles to larger vesicles and lysosomes. These data, however, still confirm the entry of virus by endocytosis and the transport into lysosomes (Talbot & Vance, 1982). * Cell cultures were cooled to 0 °C and MHV 3 was allowed to bind for 30 min at three different virus doses (10, 50 and 250 p.f.u./5 x 105 cells) in the absence (control) or presence of 30 mM-NH,C1.…”

Section: Discussionmentioning

confidence: 65%

“…Recently, Talbot & Vance (1982) suggested that NH4C1 appeared to act by blocking a step between binding of Sindbis virus on receptors of the plasma membrane and penetration into lysosomes rather than inhibiting the fusion of the virus envelope and lysosomal membranes. Alternatively, NH4CI may have prevented the delivery of viruses contained in small endocytic vesicles to larger vesicles and lysosomes.…”

Section: Discussionmentioning

confidence: 99%

Entry of Mouse Hepatitis Virus 3 into Cells

Krzystyniak

Dupuy

1984

Journal of General Virology

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SUMMARYThe involvement of lysosomes in infection by mouse hepatitis virus 3 (MHV3) was studied. L cells were infected with MHV3 in the presence of NH4C1 or chloroquine, weak bases which increase the intralysosomal pH and impair lysosomal functions. NH4CI significantly inhibited virus-induced cytopathic effects and MHV3 replication, but did not prevent the attachment of 3H-labelled virus. No inhibition of MHV3 replication by NHaC1 and chloroquine was observed when tysosomotropic agents were added later than 3 h post-infection, suggesting the direct involvement of lysosomes in release of the viral genome into cytoplasm. These results, together with the lack of antibody-mediated immune lysis of MHV3-infected cells, suggest that MHV3 entered cells by an endocytic pathway (viropexis) followed by internalization into cellular lysosomes.

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Biochemical studies on the entry of sindbis virus into BHK-21 cells and the effect of NH4Cl

Cited by 24 publications

References 20 publications

Inhibition of Endosome-Lysosome System Acidification Enhances Porcine Circovirus 2 Infection of Porcine Epithelial Cells

Inhibition of Endosome-Lysosome System Acidification Enhances Porcine Circovirus 2 Infection of Porcine Epithelial Cells

Ultrastructural and Biochemical Study of Frog Virus 3 Uptake by BHK-21 Cells

Entry of Mouse Hepatitis Virus 3 into Cells

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