Biochemical responses in freshwater fish after exposure to water-soluble fraction of gasoline

Bettim, Franciele Lima; Galvan, Gabrieli Limberger; Cestari, Marta Margarete; Yamamoto, Carlos Itsuo; Assis, Helena C. Silva de

doi:10.1016/j.chemosphere.2015.09.109

Cited by 29 publications

(4 citation statements)

References 36 publications

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“…Similar experimental results had been reported in previous studies (Costa et al, 2012;Nam et al, 2017). After the brain and muscle were exposed to soluble components of crude oil, the changes in AchE activity were not obvious compared to other organs (Bettim et al, 2016). In addition, the toxicity induced by CYP1A indicated the chemical exposure and preferential effects in various biological tissues (Whyte et al, 2000).…”

Section: Correlation Analysissupporting

confidence: 87%

Long-term Exposure to Phenanthrene-Induced Gene Expressions and Enzyme Activities of Cyprinus Carpio Below the Safe Concentration

Kang

Cao

et al. 2021

Preprint

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Phenanthrene (PHE) is a typical food chain biomagnified compound which endangers human health and is generally accumulated from marine lives. Previous PHE-stressed Carp acute toxicity test showed that the safe concentration of PHE to carp was 1.12 mg/L. In this study, the carp was long-term exposed to PHE below safe concentration up to 25 days. The gene expression levels and cytochrome P450 (CYP1A/EROD (7-Ethoxylesorufin O-deethylase)) and glutathione S-transferase (GST) activities were determined in the carp liver and brain tissues. The results showed that both the CYP1A mRNA expression and EROD activity in the liver were continuously stimulated after induction with the increase in exposure time and exposure concentration. However, with the increase of PHE concentration, GST mRNA expression in the liver was firstly induced and then inhibited and the induction was significant in the treatment with 0.1 mg/L PHE in the 15th day (almost 2-fold). In the brain, after the 15th day, GST mRNA expression was suppressed, but GST activity was induced. Correlation analysis results showed that the CYP1A mRNA expression was significantly correlated with the activity of EROD in both tissues (liver, r = 0.602, P < 0.01; brain, r = 0.508, P < 0.01), but the correlation between GST mRNA expression and GST activity was poor (liver, r = 0.385, P < 0.01; brain, r = 0.293, P < 0.01). This experiment revealed the self-regulation mechanism of carp exposed to lower than safe concentrations of PHE for a long time, indicating the toxicological risk of PHE in the ecosystem.

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Section: Correlation Analysissupporting

confidence: 87%

Long-term Exposure to Phenanthrene-Induced Gene Expressions and Enzyme Activities of Cyprinus Carpio Below the Safe Concentration

Kang

Cao

et al. 2021

Preprint

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“…While some fish species exposed to phenanthrene presented no GST and CAT induction compared to control groups (Oliveira et al, 2008), others species showed induction only for GST when exposed to WSFDO for 96 h (Mattos, 2010), and for 96 h and 15 days (Simonato et al, 2008). In the contrary, reduction of GST in liver of Astyanax altiparanae was found after 96 h exposure to water soluble fraction of gasoline (Bettim et al, 2016). CAT activity, in turn, was not induced in fish exposed to crude oil and WSFDO (Simonato et al, 2008).…”

Section: Laboratory Assaymentioning

confidence: 74%

Effects of diesel oil and environmental quality on the enzymatic activities of a tropical estuarine catfish and implications for contamination assessment

Berenger¹,

Moraes²,

Oliveira³

et al. 2017

EEC

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The contamination of coastal areas by petroleum and its derivatives is a relevant issue based on large-scale production and consumption. This study presents field and laboratory surveys on the detoxification enzymes on the estuarine/coastal catfish Genidens genidens to understand the enzymatic responses to oil derivatives and environmental conditions. Field catalase (CAT) and glutathione S-tranferase (GST) measurements suggest oxidative stress and acting repair mechanisms in fishes, but were not induced by diesel oil as indicated by laboratory studies. On the other hand, higher ethoxyresorufin-O-deethylase (EROD) activities following higher polycyclic aromatic hydrocarbon (PAH) concentrations in the estuarine waters suggested fish responsiveness to oil contamination, mainly due to the fact that such EROD values were similar to the magnitude measured in fish during a 24-h exposure to diesel oil in the laboratory. However, other environmental estuarine conditions may also act on field EROD activities since PAHs concentrations in the water-soluble fraction of diesel oil (WSFDO) were higher than those measured in estuarine waters.

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“…Similar experimental results had been reported in previous studies [ 7 , 45 ]. After the brain and muscle were exposed to soluble components of crude oil, the changes in AchE activity were not obvious compared to other organs [ 46 ]. In addition, the toxicity induced by CYP1A indicated the chemical exposure and preferential effects in various biological tissues [ 38 ].…”

Section: Resultsmentioning

confidence: 99%

Long-Term Exposure to Phenanthrene Induced Gene Expressions and Enzyme Activities of Cyprinus carpio below the Safe Concentration

Kang

Zhao

et al. 2022

IJERPH

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Phenanthrene (PHE) is a typical compound biomagnified in the food chain which endangers human health and generally accumulates from marine life. It has been listed as one of the 16 priority PAHs evaluated in toxicology. In order to evaluate the changes of CYP1A GST mRNA expression and EROD GST enzyme activity in carp exposed to lower than safe concentrations of PHE. Long-term exposure of carp to PHE at lower than safe concentrations for up to 25 days. The mRNA expression level and cytochrome P450 (CYP1A/EROD (7-Ethoxylesorufin O-deethylase)) and glutathione S-transferase (GST) activity were measured in carp liver and brain tissue. The results showed that PHE stress induced low-concentration induction and high-concentration inhibition of CYP1A expression and EROD enzyme activity in the liver and brain of carp. In both two organs, GST enzyme activity was also induced. However, the expression of GST mRNA was first induced and then inhibited, after the 15th day. These results indicate that long-term exposure to PHE at lower than safe concentrations still poses a potential threat to carp’s oxidase system and gene expression.

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Biochemical responses in freshwater fish after exposure to water-soluble fraction of gasoline

Cited by 29 publications

References 36 publications

Long-term Exposure to Phenanthrene-Induced Gene Expressions and Enzyme Activities of Cyprinus Carpio Below the Safe Concentration

Long-term Exposure to Phenanthrene-Induced Gene Expressions and Enzyme Activities of Cyprinus Carpio Below the Safe Concentration

Effects of diesel oil and environmental quality on the enzymatic activities of a tropical estuarine catfish and implications for contamination assessment

Long-Term Exposure to Phenanthrene Induced Gene Expressions and Enzyme Activities of Cyprinus carpio below the Safe Concentration

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