Biochemical Response to D-Glucosone in Terms of Motility of the Stomach, Insulin Secretion, and Sweetness

Abstract

D-Glucosone (D-arabino-hexos-2-ulose) was prepared by use of an immobilized glucose 2-oxidase-catalase gel, and the biochemical response to D-glucosone by three kinds of glucose-recognizing systems in higher animals was studied. The enhanced motility of rat stomach caused by glucopenia was reduced by carotid arterial injection of D-glucosone like in the case of D-glucose. D-Glucosone (16.7 mM) showed no effect on the secretion of insulin from the pancreatic islets of the rat, and the secretion of insulin caused by D-glucose (16.7 mM) was markedly depressed by the addition of D-glucosone (16.7 mM). The relative sweetness of D-glucosone, compared with that of 5 % sucrose as 1.0, was estimated to be 0.4-0.6, which is similar to that of D-glucose. These results suggest that D-glucosone is a useful model compound for D-glucose to investigate the biochemical mechanism of glucose-recognizing systems.

Determination of monosaccharides in cellulosic hydrolyzates using immobilized pyranose oxidase in a continuous amperometric analyzer

“…Pyranose oxidase, a flavoprotein with an assumed mechanism similar to glucose oxidase, has only twice been described for analytical use and then with soluble enzyme (15,16). In a recent study we demonstrated the immobilization of this enzyme with results of significant increased stability at higher temperature and prolongation of operational and storage life (11).…”