Biochemical Properties of Water-Soluble Otolith Proteins and the Immunobiochemical Detection of the Proteins in Serum and Various Tissues in the Tilapia &lt;i&gt;Oreochromis niloticus&lt;/i&gt;

Sasagawa, Tadashi; Mugiya, Yasuo

doi:10.2331/fishsci.62.970

Cited by 28 publications

(27 citation statements)

References 7 publications

(1 reference statement)

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“…It is important to clarify the functional significance of organic matrices to otolith mineralization. Although amino acid composition of several otolith organic matrices has been reported (Degens et al 1969, Baba et al 1991, Asano & Mugiya 1992, Sasagawa & Mugiya 1996, only 1 matrix protein of the teleost otolith has been identified and characterized (Murayama et al 2000). Further identification of otolith organic matrices is necessary to allow the study of the functions of otolith organic matrices.…”

Section: +mentioning

confidence: 99%

“…Resale or republication not permitted without written consent of the publisher organic matrix including proteins, carbohydrates and lipids (Mugiya 1968, Degens et al 1969, Baba et al 1991, Asano & Mugiya 1992, Sasagawa & Mugiya 1996, Takagi & Takahashi 1999, Murayama et al 2000. Otoliths are bathed in endolymph within the inner ear sacs.…”

Section: Abstract: Otolith · Endolymph · Chemical Composition · Aragmentioning

confidence: 99%

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Otolith formation and endolymph chemistry: a strong correlation between the aragonite saturation state and pH in the endolymph of the trout otolith organ

Takagi¹

2002

Mar. Ecol. Prog. Ser.

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Section: +mentioning

confidence: 99%

Section: Abstract: Otolith · Endolymph · Chemical Composition · Aragmentioning

confidence: 99%

Otolith formation and endolymph chemistry: a strong correlation between the aragonite saturation state and pH in the endolymph of the trout otolith organ

Takagi¹

2002

Mar. Ecol. Prog. Ser.

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“…Otolith matrix proteins probably have a crucial function in mediating or inhibiting aragonite deposition, and diel fluctuations in deposition of the matrix proteins lead to fluctuations in calcification. Although several reports have tried to explain the interaction between the otolith organic matrices and Ca 2+ or CaCO 3 (Baba et al 1991, Wright 1991, Asano & Mugiya 1992, Sasagawa & Mugiya 1996, Borelli et al 2001), the precise nature of the relationship between them remains unknown. In order to understand the mechanism of increment formation more accurately, we would have to clarify the function of each component of the organic matrices more precisely.…”

mentioning

confidence: 99%

Diel changes in endolymph aragonite saturation rate and mRNA expression of otolith matrix proteins in the trout otolith organ

Takagi¹,

Tohse²,

Murayama³

et al. 2005

Mar. Ecol. Prog. Ser.

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In the teleost fish otolith, the alternate deposition of CaCO 3 -rich and protein-rich layers results in the formation of daily increments. In order to clarify the mechanism of daily increment formation, a precise understanding of the relationship between ionic and organic controls of otolith growth is essential. In the present study, we studied diel variations in the aragonite saturation rate (S a ) of the endolymph and the mRNA expression of 2 major otolith matrix proteins, OMP-1 and otolin-1, in the saccular tissue. A new technique for simultaneously quantifying endolymph S a and mRNA expression in the saccular tissue from an individual rainbow trout was developed. Endolymph Ca 2+ levels, CO 2 partial pressure (P CO 2 ) and pH were simultaneously measured using an automatic pH/gas/electrolyte analyzer, and the S a was calculated. Total RNA was isolated from sacculi of individual fish after the endolymph was obtained. cDNAs were synthesized and used to quantify OMP-1 and otolin-1 mRNA expression levels by real-time PCR. Significant diel variations were observed in endolymph pH and P CO 2 levels in an antiphasic manner. Endolymph S a did not exhibit significant diel variations and was maintained at a value of more than 2.0, indicating that the endolymph was kept supersaturated with respect to aragonite during the day-night cycle. Expression of otolin-1 mRNA had apparent diel variations with high levels at night, whereas that of OMP-1 mRNA was almost constant. These data strongly suggest an organic control of daily increment formation in the otolith. The most probable candidate protein for daily increment formation is otolin-1.KEY WORDS: Calcification · mRNA expression · Endolymph · Daily increment · Aragonite · Otolith · Rainbow trout Resale or republication not permitted without written consent of the publisherMar Ecol Prog Ser 294: [249][250][251][252][253][254][255][256] 2005 and the dark one is referred to as the D-zone (Kalish et al. 1995). Morphological examinations have revealed that the D-zones contain more organic matrices and less calcium carbonate relative to the L-zones (Mugiya & Muramatsu 1982, Watabe et al. 1982, Morales-Nin 1987; therefore, the alternate manner in which the deposition of mineral-rich and organic-matrix-rich layers occurs is manifested as daily increments. Indeed, Mugiya (1987) reported the discrete antiphasic diel cycles of 45 Ca and 3 H-glutamic-acid uptake into the otoliths in the rainbow trout in vitro and suggested that the daily increments of otoliths were formed by the antiphasic deposition of calcium and organic matrices. Daily variations in otolith calcification have also been demonstrated in the goldfish (Mugiya et al. 1981, Tohse & Mugiya 2002 and in the Atlantic salmon (Wright et al. 1992).The mechanism of these diel variations in otolith calcification and the deposition of the organic matrix is unknown. The otolith can be defined as acellular tissue that grows in an inner ear sacculus filled with endolymph. Consequently, endolymph must contain all the pr...

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“…Driven by thermodynamic controls, aqueous calcium (Ca 2+ ) and carbonate ions (CO 3 2-) in the endolymphatic fluid bind together to form calcium carbonate, which is deposited on the otolith (Romanek and Gauldie, 1996). Proteins extracted from tilapia (Oreochromis niloticus) otolith chambers reveal high calcium-binding capacity, which promotes otolith calcification (Sasagawa and Mugiya, 1996).…”

Section: Introductionmentioning

confidence: 99%

Plasma membrane calcium ATPase required for semicircular canal formation and otolith growth in the zebrafish inner ear

Cruz

Shiao

Liao

et al. 2009

Journal of Experimental Biology

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SUMMARYFish otoliths consist of >90% calcium carbonate, the accretion of which depends on acellular endolymph. This study confirms the presence of plasma membrane calcium ATPase 1a isoform (Atp2b1a) in the auditory and vestibular system of a teleost fish. As shown by in situ hybridization, zebrafish atp2b1a is expressed mainly in larval otic placode and lateral-line neuromast as well as in the hair cells within the adult zebrafish inner ear chamber. Zebrafish atp2b1a knockdown by antisense morpholinos reduced the number of hair cells and produced malformation of semicircular canals and smaller otoliths. These defects coincide with unbalanced body orientation. The formation of smaller otoliths in atp2b1a morphants may stem from an impairment of calcium supply in the endolymph. However, otolith formation persists in most morphants, suggesting that other zebrafish Atp2b isoforms or paracellular pathways may also transport calcium into the endolymph. These results suggest that Atp2b1a plays an important role for normal development of the auditory and vestibular system as well as calcium transport in the inner ear of zebrafish. Supplementary material available online at

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Biochemical Properties of Water-Soluble Otolith Proteins and the Immunobiochemical Detection of the Proteins in Serum and Various Tissues in the Tilapia <i>Oreochromis niloticus</i>

Cited by 28 publications

References 7 publications

Otolith formation and endolymph chemistry: a strong correlation between the aragonite saturation state and pH in the endolymph of the trout otolith organ

Otolith formation and endolymph chemistry: a strong correlation between the aragonite saturation state and pH in the endolymph of the trout otolith organ

Diel changes in endolymph aragonite saturation rate and mRNA expression of otolith matrix proteins in the trout otolith organ

Plasma membrane calcium ATPase required for semicircular canal formation and otolith growth in the zebrafish inner ear

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