Biochemical properties of the cell wall in the <i>Arabidopsis</i> mutant <i>bor1‐1</i> in relation to boron nutrition

Noguchi, Kyotaro; Ishii, Tadashi; Matsunaga, Toshiro; Kakegawa, Koichi; Hayashi, Hiroaki; Fujiwara, Toru

doi:10.1002/jpln.200390025

Cited by 32 publications

(24 citation statements)

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“…Studies have shown that B in cell walls is present as a borate ester of RG-II, a polysaccharide in pectin. In the bor1-1 mutant of Arabidopsis, B concentrations in the cell wall of shoot tissues were lower than those in the wild type (WT); however, the sugar composition of cell wall fractions was similar with WT, suggesting that the polysaccharide composition in the cell wall was not strongly affected by the bor1-1 mutation (Noguchi et al 2003). Unlike the bor1-1 mutant, the differences in the concentration of cell wall pectin have been observed in B. napus lines of contrasting B efficiency.…”

Section: B Translocation and Utilizationmentioning

confidence: 97%

Physiological and genetic responses to boron deficiency inBrassica napus: A review

Zhang

Zhao

Shi

et al. 2014

Soil Science and Plant Nutrition

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Boron (B) is an essential microelement for the growth and development of plants, and B deficiency affects many biochemical and physiological processes. Brassica napus L. has a high demand for B and is extremely sensitive to B deficiency. Seed yields and oil quality of B. napus are often limited by the low availability of B in soils. Developing new cultivars of B. napus with high B efficiency is therefore required, which requires a greater understanding of responses to B deficiency. Significant genotypic differences in response to low soil B have been observed among varieties of B. napus. B-efficient genotypes can grow and yield normally and usually have a larger root system than B-inefficient genotypes at low B conditions. The mechanisms for B efficiency in B. napus are attributed to B absorption, transportation and utilization. In addition, the cell wall component plays an important role in the tolerance of B. napus to B deficiency, and the B-efficient line presents fewer B-binding sites in the cell walls compared with the B-inefficient line. Genetic and proteomic analyses in B. napus revealed the modulation of a complex network in response to B deficiency. This review gives a comparative overview of the physiological and genetic responses to B deficiency in B. napus and discusses the possible underlying mechanisms of B efficiency.

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Section: B Translocation and Utilizationmentioning

confidence: 97%

Physiological and genetic responses to boron deficiency inBrassica napus: A review

Zhang

Zhao

Shi

et al. 2014

Soil Science and Plant Nutrition

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“…Shoot growth in bor1-1 plants is severely inhibited under low-B conditions, but root growth is not (Takano et al, 2001). Borate RG-II cross linking in shoots is reduced in bor1-1 under the low-B condition, correlating with the reduced B concentrations in total shoots and shoot cell wall fraction (Noguchi et al, 2003). The bor1-1 mutant has no apparent defect in B uptake by roots but is impaired in efficient root-toshoot translocation of the mineral under low-B conditions (Noguchi et al, 2000;Takano et al, 2002).…”

mentioning

confidence: 88%

Roles of BOR2, a Boron Exporter, in Cross Linking of Rhamnogalacturonan II and Root Elongation under Boron Limitation in Arabidopsis

et al. 2013

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Boron (B) is required for cross linking of the pectic polysaccharide rhamnogalacturonan II (RG-II) and is consequently essential for the maintenance of cell wall structure. Arabidopsis (Arabidopsis thaliana) BOR1 is an efflux B transporter for xylem loading of B. Here, we describe the roles of BOR2, the most similar paralog of BOR1. BOR2 encodes an efflux B transporter localized in plasma membrane and is strongly expressed in lateral root caps and epidermis of elongation zones of roots. Transfer DNA insertion of BOR2 reduced root elongation by 68%, whereas the mutation in BOR1 reduced it by 32% under low B availability (0.1 mM), but the reduction in shoot growth was not as obvious as that in the BOR1 mutant. A double mutant of BOR1 and BOR2 exhibited much more severe growth defects in both roots and shoots under B-limited conditions than the corresponding single mutants. All single and double mutants grew normally under B-sufficient conditions. These results suggest that both BOR1 and BOR2 are required under B limitation and that their roles are, at least in part, different. The total B concentrations in roots of BOR2 mutants were not significantly different from those in wild-type plants, but the proportion of cross-linked RG-II was reduced under low B availability. Such a reduction in RG-II cross linking was not evident in roots of the BOR1 mutant. Thus, we propose that under B-limited conditions, transport of boric acid/borate by BOR2 from symplast to apoplast is required for effective cross linking of RG-II in cell wall and root cell elongation.

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“…In the bor1-1 mutant, which is defective in root-to-shoot translocation of B, growth retardation was observed particularly in shoots (Takano et al, 2001). Because it has been proven that B functions in the structural integrity of the cell wall by dimerization of RG-II , a lower rate of RG-II dimerization in shoot tissues was thought to be responsible for the growth retardation in bor1-1 plants (Noguchi et al, 2003). Similarly, the growth retardation in nip5;1 shoots is best explained by a lower rate of B delivery to the shoots ( Figures 7B and 7D) derived from a lower capacity for B uptake into roots ( Figures 7A and 7C).…”

Section: A Role For Nip5;1 In Plant Growth and Development Under B LImentioning

confidence: 99%

The Arabidopsis Major Intrinsic Protein NIP5;1 Is Essential for Efficient Boron Uptake and Plant Development under Boron Limitation

et al. 2006

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Boron (B) is essential in plants but often present at low concentrations in the environment. To investigate how plants survive under conditions of B limitation, we conducted a transcriptome analysis and identified NIP5;1, a member of the major intrinsic protein family, as a gene upregulated in B-deficient roots of Arabidopsis thaliana. Promoter-b-glucuronidase fusions indicated that NIP5;1 is strongly upregulated in the root elongation zone and the root hair zone under B limitation, and green fluorescent protein-tagged NIP5;1 proteins localized to the plasma membrane. Expression in Xenopus laevis oocytes demonstrated that NIP5;1 facilitated the transport of boric acid in addition to water. Importantly, two T-DNA insertion lines of NIP5;1 displayed lower boric acid uptake into roots, lower biomass production, and increased sensitivity of root and shoot development to B deficiency. These results identify NIP5;1 as a major plasma membrane boric acid channel crucial for the B uptake required for plant growth and development under B limitation.

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Biochemical properties of the cell wall in the Arabidopsis mutant bor1‐1 in relation to boron nutrition

Cited by 32 publications

References 0 publications

Physiological and genetic responses to boron deficiency inBrassica napus: A review

Physiological and genetic responses to boron deficiency inBrassica napus: A review

Roles of BOR2, a Boron Exporter, in Cross Linking of Rhamnogalacturonan II and Root Elongation under Boron Limitation in Arabidopsis

The Arabidopsis Major Intrinsic Protein NIP5;1 Is Essential for Efficient Boron Uptake and Plant Development under Boron Limitation

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