Biochemical properties of the alkaline lipase of Bacillus flexus XJU-1 and its detergent compatibility

Abstract

The possibility of using Bacillus flexus XJU-1 lipase in detergent preparations was studied. The enzyme was monomeric protein as confirmed by liquid chromatography-mass spectrometry and its molecular weight was 15.95 kDa. The lipase showed optimum activity at pH 10.0 and was 100% stable for 24 h at pH 10.0 and 11.0. It exhibited maximum activity at 70• C and retained more than 70% of the initial activity at 60, 70 and 80• C for 24 h. The activity was stimulated by Ca 2+ , Ba 2+ , Mg 2+ and Co 2+ , whereas 50% of the initial activity was lost with Fe 3+ and Hg 2+ . The activity was inhibited by 10 mM N-bromosuccinimide and tosyl-L-lysylchloromethylketone, while N-ethylmaleimide, phenylmethylsulphonylfluoride and urea did not show any effect. The enzyme significantly hydrolysed olive, cottonseed, sunflower, groundnut, and gingelly oils. With p-nitrophenyl palmitate, Vmax and Km were 62.5 U/mL and 2.25 mM, respectively. The lipase maintained its stability in Tween-80, Triton-100 and H2O2 at 1%, but an activation of 10% and a reduction of 15% in relative activity were observed with NaClO and sodium dodecyl sulphate, respectively. The enzyme retained maximum storage stability for 20 days at −20, 4 and 30• C. In the presence of 0.7% (w/v) Ariel, Henko, Super wheel, Tide plus and Rin, a retention of more than 84.90% initial activity was recorded after 24 h at 60• C. The supplementation of the lipase to the detergents improved the olive oil stain removal. These properties suggested the present enzyme as a potential additive for detergent preparations.

Recombinant Lipases and Phospholipases and Their Use as Biocatalysts for Industrial Applications

“…ESW [ 133 ], Bacillus sp. LBN 2 [ 134 ], and Bacillus flexus XJU-1 [ 135 ]. Alkaline lipases have also been isolated from the yeasts Humicola lanuginosa , Trichoderma lanuginosus , Trichosporon asahii [ 131 ] and, recently, Rhodosporidium babjevae BD19 [ 136 ].…”

Characterization of Lipases from a Vegetable Oil Contaminated Soil Fungal Isolates

“…Soils particularly the ones contaminated with vegetable oil have been reported to contain lipolytic enzymes which often degrade the fats and oils in them [9]. Filamentous fungi of the many species of genera Mucor, Candida [10], Aspergillus, Rhizopus, penicillium and trichoderma have been reported to be the most prospective lipase producers [11] but despite this, many microorganisms having the ability to produce lipases which are able to breakdown fats and oils are yet to be isolated, identified and characterized [6].…”

Thermostable acidic lipase of Bacillus glycinifermentans-MK840989 isolated from contaminated environment; its optimization, purification and exploring potential applications

“…can enhance the enzymatic activity. 14 The enzyme purification involves different steps such as ammonium sulfate precipitation, chromatographic techniques including hydrophobic interaction and ion exchange methods. 15 Since high temperature directly influences the enzyme properties e.g.…”

“…stability, reaction rate, solubility and lesser substrate viscosity as well as contamination, temperature-stable enzyme is desired. 14,16 Moreover, the presence of metal ions is reported to improve its lipolytic activity. 14,17 In this study, an effort is made to explore the lipase producing bacterium from indigenous environment.…”

“…14,16 Moreover, the presence of metal ions is reported to improve its lipolytic activity. 14,17 In this study, an effort is made to explore the lipase producing bacterium from indigenous environment. Here, we report the isolation of lipase producing bacterium, its characterization and growth optimization on different substrates.…”