Biochemical properties of cofactor and coenzyme metabolism in porcine oviductal epithelial cells – a microarray study

Abstract

The oviduct is a key organ responsible for ultimate oocytes maturation, transport of gametes, sperm capacitation, fertilization, as well as early embryo development. Its innermost layer, oviductal epithelium, represents a highly dynamic structure which undergoes changes in response to different physiological and pathological processes. Previously, the expression profile of genes involved in several important processes in porcine oviductal epithelial cells (OECs) during long-term primary in vitro culture. The present study further characterizes the porcine OECs model using Affymetrix microarray assay and it analyzes gene expression changes observed on the 7th, 15th and 30th day of culture. 25 genes belonging to “coenzyme metabolic process”, “cofactor biosynthetic process” and “cofactor metabolic process” GO BP terms were differentially expressed in culture. The most up-regulated genes were ALDH1L2, P2RX7, PANK1, ACSS2, SCD, AASS and PDK3. In contrast, several genes appeared to be significantly down-regulated, e.g. ACSL4 and HAAO. Considering the biological roles of the most regulated genes, it can be concluded that these changes may indicate the increased metabolic and proliferation activity of studied cells in primary in vitro culture.Running title: Cofactor and coenzyme metabolism in porcine oviductal epithelial cells

Cortical Granule Distribution and Expression Pattern of Genes Regulating Cellular Component Size, Morphogenesis, and Potential to Differentiation are Related to Oocyte Developmental Competence and Maturational Capacity In Vivo and In Vitro

“…RNA samples were treated with DNase I and reverse transcribed (RT) into cDNA. RT-qPCR was performed in a real-time LightCycler PCR detection system (Roche Diagnostics GmbH, Mannheim, Germany) using SYBRR Green I as a detection dye, with target cDNA quantified using a relative quantification method [29]. The relative abundance of all tested genes transcripts in each sample was normalized to internal standards (PBGD, β-actin, 18S rRNA).…”

Human Ovarian Granulosa Cells Isolated during an IVF Procedure Exhibit Differential Expression of Genes Regulating Cell Division and Mitotic Spindle Formation

“…Each GO term and KEGG pathway was considered significantly enriched if it contained at least 5 differentially expressed genes, with p < 0.05. An RT-qPCR statistical analysis was performed using the Real Statistics Resource Pack for MS Excel 2016, also using the abovementioned methods (Microsoft Corporation, Redmond, WA, USA) [15,27,28].…”