Biochemical Properties of Carbohydrate-Active Enzymes Synthesized by Penicillium chrysogenum Using Corn Straw as Carbon Source

Silva, Luísa de M. B.; Gomes, Tainah C.; Ullah, Sadia Fida; Ticona, Alonso R. P.; Hamann, Pedro Ricardo Vieira; Noronha, Eliane Ferreira

doi:10.1007/s12649-019-00589-x

Cited by 11 publications

(5 citation statements)

References 52 publications

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“…For zymogram analysis, precipitated XynGH10 was directly applied with sample buffer into a 12% SDS‐PAGE copolymerised with 0.1% (wt/vol) oat spelt xylan. Renaturing and washing steps were carried out except for the activity temperature at 65°C (Silva et al, 2020).…”

Section: Methodsmentioning

confidence: 99%

“…The colorimetric reaction was read using a spectrophotometer at 540 nm, and one unit of enzymatic activity was defined as the amount of enzyme required to release 1 μmol of the reducing sugar. The standard curve was built using D-xylose (Silva et al, 2020). (Figure 2).…”

Section: Protein Extraction and Immunoblot Analysismentioning

confidence: 99%

“…CP, coat protein; FMDV, foot-and-mouth disease virus; GFP, green fluorescent protein buffer into a 12% SDS-PAGE copolymerised with 0.1% (wt/vol) oat spelt xylan. Renaturing and washing steps were carried out except for the activity temperature at 65 C(Silva et al, 2020).2.5 | Endo-β-1,4-xylanase activity quantificationXylanase (endo-β-1,4-xylanase) activity present in plant supernatant containing recombinant xylanase, or in purified XynGH10 were detected by mixing 50 μL of samples with 100 μL of 1% oat spelt xylan (wt/vol) (Sigma-Aldrich) dissolved in 50 mM sodium acetate buffer with pH 5.0 and incubated in a water bath at 65 C for 30 min.…”

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confidence: 99%

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Development of a heterologous gene expression vector in plants based on an infectious clone of a tobravirus, pepper ringspot virus

Tavares‐Esashika

Campos

Maeda

et al. 2022

Annals of Applied Biology

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Heterologous gene expression systems are important tools for biotechnology, especially due to the increasing demands for mass production of pharmaceutical proteins.In this study, we assessed the use of the infectious clone of a tobravirus, pepper ringspot virus (PepRSV), as a heterologous gene expression tool in Nicotiana benthamiana plants. The insert region of gene of interest was evaluated in the infectious clone of PepRSV using the reporter gene encoding the green fluorescent protein (GFP). The insertion of the GFP gene in the coat protein gene region resulted in a higher protein yield than the previous system. Then, the reporter gene was replaced by the xylanase gene, and enzymatically active xylanase was produced. This study provides an efficient tool for the production of heterologous proteins using a plant system.

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Section: Methodsmentioning

confidence: 99%

Section: Protein Extraction and Immunoblot Analysismentioning

confidence: 99%

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confidence: 99%

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Development of a heterologous gene expression vector in plants based on an infectious clone of a tobravirus, pepper ringspot virus

Tavares‐Esashika

Campos

Maeda

et al. 2022

Annals of Applied Biology

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“…At present, industrial-scale xylanase production is largely dependent on microorganisms such as species in the genera Trichoderma and Aspergillus [ 15 , 16 , 17 ]. However, many species of the genus Penicillium were reported to have significant potential for plant biomass degradation and are therefore considered as effective xylanase-producers [ 18 , 19 , 20 ]. Among these, Penicillium oxalicum is regarded as one of the most promising fungi capable of producing a wide range of industrially relevant enzymes [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

A High-Quality Genome Sequence of the Penicillium oxalicum 5-18 Strain Isolated from a Poplar Plantation Provides Insights into Its Lignocellulose Degradation

Zhu

et al. 2023

IJMS

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Studies on the degradation of plant cell wall polysaccharides by fungal extracellular enzymes have attracted recent attention from researchers. Xylan, abundant in hemicellulose, that play great role in connection between cellulose and lignin, has seen interest in its hydrolytic enzymatic complex. In this study, dozens of fungus species spanning genera were isolated from rotting leaves based on their ability to decompose xylan. Among these isolates, a strain with strong xylanase-producing ability was selected for further investigation by genome sequencing. Based on phylogenetic analysis of ITS (rDNA internal transcribed spacer) and LSU (Large subunit 28S rDNA) regions, the isolate was identified as Penicillium oxalicum. Morphological analysis also supported this finding. Xylanase activity of this isolated P. oxalicum 5-18 strain was recorded to be 30.83 U/mL using the 3,5-dinitro-salicylic acid (DNS) method. Further genome sequencing reveals that sequenced reads were assembled into a 30.78 Mb genome containing 10,074 predicted protein-encoding genes. In total, 439 carbohydrate-active enzymes (CAZymes) encoding genes were predicted, many of which were associated with cellulose, hemicellulose, pectin, chitin and starch degradation. Further analysis and comparison showed that the isolate P. oxalicum 5-18 contains a diverse set of CAZyme genes involved in degradation of plant cell wall components, particularly cellulose and hemicellulose. These findings provide us with valuable genetic information about the plant biomass-degrading enzyme system of P. oxalicum, facilitating a further exploration of the repertoire of industrially relevant lignocellulolytic enzymes of P. oxalicum 5-18.

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“…Many straw-degrading microorganisms are well explored for straw degradation, including Trichoderma, Chaetomium, Penicillium, Aspergillus, Acremonium, Rhizopus, etc (19,24,25,28,4). The most widely used cellulase producing strain is Trichoderma reesei.…”

Section: Introductionmentioning

confidence: 99%

Cellulase Production by a Newly Isolated Soil Fungus Penicillium Griseofulvum A2

Guan

Liu

Cai

et al. 2021

Preprint

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Rice straw is one of the most abundant resources in the world. Returning straw to field is an effective method facilitating sustainable agricultural production in many countries. However, the crop residues cannot be degraded easily due to recalcitrant structure and composition of lignocellulosic material. A straw-degrading fungus Penicillium griseofulvum A2 was isolated from rice straw culture medium and identified by morphology and ITS rRNA gene sequencing. Enzyme-producing condition of A2 was optimized, including nitrogen source ((NH4)2SO4, NH4Cl, urea, and peptone), pH value (5.0 to 7.5), and incubation time (1 to 20 d). Results showed that P. griseofulvum A2 produced the highest CMCase (33.97 ± 0.33 U/mL) utilizing urea as nitrogen source and growing at pH 7.0-7.5 after 15 d cultivation. While, NH4Cl was more suitable for β-glucosidase (15.40 ± 1.77 U/mL) production under pH 7.0 condition after 10 d cultivation. The isolated fungus P. griseofulvum A2 is a good potential strains to decomposed rice straw.

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Biochemical Properties of Carbohydrate-Active Enzymes Synthesized by Penicillium chrysogenum Using Corn Straw as Carbon Source

Cited by 11 publications

References 52 publications

Development of a heterologous gene expression vector in plants based on an infectious clone of a tobravirus, pepper ringspot virus

Development of a heterologous gene expression vector in plants based on an infectious clone of a tobravirus, pepper ringspot virus

A High-Quality Genome Sequence of the Penicillium oxalicum 5-18 Strain Isolated from a Poplar Plantation Provides Insights into Its Lignocellulose Degradation

Cellulase Production by a Newly Isolated Soil Fungus Penicillium Griseofulvum A2

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