Biochemical Properties of Black Drum and Sheepshead Seabream Skin Collagen

Abstract

Acid-soluble collagen (ASC) and pepsin solubilized collagen (PSC) were isolated from the skins of black drum (Pogonias cromis) and sheepshead seabream (Archosargus probatocephalus) harvested in the Gulf of Mexico coastal waters. The yields of ASCs on dry basis from black drum and sheepshead were estimated at 2.3 and 2.6%, and the yields of PSCs were 15.8 and 29.3%, respectively. Analyses of molecular weight profile, amino acid composition, and secondary structure showed that the skin collagens from both species were typical type-I collagen. The molecular mass of alpha(1) and alpha(2) subunits, as determined by SDS-PAGE using Tris-Acetate gels, was 127 kDa and 116 kDa, respectively. The amino acid composition of ASC and PSC for both species was closer to calf skin ASC than to cod skin ASC. Thermal denaturation temperatures, measured by melting point using circular dichroism, gave the following values: black drum ASC, 34.2 degrees C; sheepshead ASC, 34.0 degrees C; black drum PSC, 35.8 degrees C; sheepshead PSC, 34.3 degrees C. The literature value for the heat stability of calf skin collagen is 36.3 degrees C. The potentials of collagens from black drum and sheepshead skins in the functional food, healthcare, and pharmaceutical industries are discussed.

Isolation and characterization of collagen from fish waste material- skin, scales and fins of Catla catla and Cirrhinus mrigala

“…This characterisation is also consistent with the findings of Nomura et al (1996), who adopted the same method. In the present study, three distinct bands of molecular weights ranging from 112 to 220 kDa in C. catla and from 114 to 210 kDa in C. mrigala were observed; these findings were similar to those reported by Ogawa et al (2003) and Yung et al (2005). The isolated collagen may be type I, which was previously observed by Kimura et al (1993) and Muyonga et al (2004), who reported that the carp skin, scale, and bone collagen was the type I based on its electrophoretic mobility.…”

“…1). These results are consistent with the findings of Ogawa et al (2003) and Zhang et al (2007), who also reported lack of a significant difference between the subunit molecular masses of the collagen from fins and scales. The C. catla fin collagen was run in wells 9 and 10 and revealed six fractions in each well with molecular weights of 70, 82, 111, 120, 180 and 201 kDa (Fig.…”

“…The hydroxyl group of the hydroxyproline plays a part in the stability of the helix by interchain hydrogen bonding via a bridging water molecule as well as direct hydrogen bonding with a carbonyl group (Wang et al 2007). However, imino acid contents of both the fish species from unicorn leather jacket skin were lower than those reported for collagen from the skin of black drum and sheep's head (Ogawa et al 2003), brown stripe red snapper (Jongjareonrak et al 2005) and bigeye snapper (Kittiphattanabawon et al 2005), which contained imino acids ranging from 190 to 221 residues/ 1,000 residues. Proline and hydroxyproline contents vary with species and their living habitat (Foegeding et al 1996;Jongjareonrak et al 2005;Kittiphattanabawon et al 2005).…”

“…Fish skin can be used as an alternative source for collagen since mammalian collagens are associated with several problems such as the outbreak of mad cow disease and the constraint for some religions, mainly Islam and Judaism (Nalinanon et al 2008). Therefore, skin collagens from several fish species such as bigeye snapper (Kittiphattanabawon et al 2005;Nalinanon et al 2007), black drum (Ogawa et al 2003), brown stripe red snapper (Jongjareonrak et al 2005), carp (Duan et al 2009), channel catfish ), Nile perch (Muyonga et al 2004), oscillate puffer fish (Nagai et al 2002), sheephead seabream (Ogawa et al 2003), threadfin bream (Nalinanon et al 2008) and yellowfin tuna (Woo et al 2008) have been extracted and characterized. Although there are many reports about collagen from skin of marine organisms, there are few studies of fish scales like the studies of Kimura et al (1991) and those of Shirai (Nomura et al 1996).…”

Detection of collagen through FTIR and HPLC from the body and foot of Donax cuneatus Linnaeus, 1758

“…But, the outbreak of highly infectious and contagious diseases such as bovine spongiform encephalopathy (BSE), transmissible spongiform encephalopathy (TSE) and food and mouth disease (FMD) in pigs and cattle has limited the use of collagen derived from them. Therefore, many scientists have been focusing to find out alternative sources for collagen and they found different organisms from marine source such as black drum (Pogonia cromis) (Ogawa et al 2003), brownstripe red snapper (Lutjanus vitta) (Jongjareonrak et al 2005), ocellate puffer fish (Takifugu rubripes) (Nagai et al 2002) and leather jacket (Odonus niger) (Muralidharan et al 2011). However, collagen from freshwater fish, were rarely reported, except for the Nile perch (Lates niloticus) (Muyonga et al 2004), grass carp (Ctenopharyngodon idella) (Zhang et al 2007) and channel catfish (Ictalurus punctaus) ).…”